Altered expression of sodium channel distribution in the dorsal root ganglion after gradual elongation of rat sciatic nerves

2009 ◽  
Vol 28 (4) ◽  
pp. 481-486 ◽  
Author(s):  
Katsunori Ohno ◽  
Atsushi Yokota ◽  
Shinji Hirofuji ◽  
Kiyoto Kanbara ◽  
Hisashi Ohtsuka ◽  
...  
Keyword(s):  
Dorsal Root Ganglion ◽  
Sodium Channel ◽  
Dorsal Root ◽  
Altered Expression ◽  
Channel Distribution ◽  
Sciatic Nerves

Rescue of α-SNS Sodium Channel Expression in Small Dorsal Root Ganglion Neurons After Axotomy by Nerve Growth Factor In Vivo

1998 ◽  
Vol 79 (5) ◽  
pp. 2668-2676 ◽  
Author(s):  
S. D. Dib-Hajj ◽  
J. A. Black ◽  
T. R. Cummins ◽  
A. M. Kenney ◽  
J. D. Kocsis ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Dorsal Root Ganglion ◽  
Sodium Channel ◽  
Dorsal Root ◽  
Sodium Current ◽  
Mrna Levels ◽  
Drg Neurons ◽  
Nerve Growth

Dib-Hajj, S. D., J. A. Black, T. R. Cummins, A. M. Kenney, J. D. Kocsis, and S. G. Waxman. Rescue of α-SNS sodium channel expression in small dorsal root ganglion neurons after axotomy by nerve growth factor in vivo. J. Neurophysiol. 79: 2668–2676, 1998. Small (18–25 μm diam) dorsal root ganglion (DRG) neurons are known to express high levels of tetrodotoxin-resistant (TTX-R) sodium current and the mRNA for the α-SNS sodium channel, which encodes a TTX-R channel when expressed in oocytes. These neurons also preferentially express the high affinity receptor for nerve growth factor (NGF), TrkA. Levels of TTX-R sodium current and of α-SNS mRNA are reduced in these cells after axotomy. To determine whether NGF participates in the regulation of TTX-R current and α-SNS mRNA in small DRG neurons in vivo, we axotomized small lumbar DRG neurons by sciatic nerve transection and administered NGF or Ringer solution to the proximal nerve stump using osmotic pumps. Ten to 12 days after pump implant, whole cell patch-clamp recording demonstrated that TTX-R current density was decreased in Ringer-treated axotomized neurons (154 ± 45 pA/pF; mean ± SE) compared with nonaxotomized control neurons (865 ± 123 pA/pF) and was restored partially toward control levels in NGF-treated axotomized neurons (465 ± 78 pA/pF). The V 1/2 for steady-state activation and inactivation of TTX-R currents were similar in control, Ringer- and NGF-treated axotomized neurons. Reverse transcription polymerase chain reaction revealed an upregulation of α-SNS mRNA levels in NGF-treated compared with Ringer-treated axotomized DRG. In situ hybridization showed that α-SNS mRNA levels were decreased significantly in small Ringer-treated axotomized DRG neurons in vivo and also in small DRG neurons that were dissociated and maintained in vitro, so as to correspond to the patch-clamp conditions. NGF-treated axotomized neurons had a significant increase in α-SNS mRNA expression, compared with Ringer-treated axotomized cells. These results show that the administration of exogenous NGF in vivo, to the proximal nerve stump of the transected sciatic nerve, results in an upregulation of TTX-R sodium current and of α-SNS mRNA levels in small DRG neurons. Retrogradely transported NGF thus appears to participate in the control of excitability in these cells via actions that include the regulation of sodium channel gene expression in vivo.

Interactions of tetramethrin, fenvalerate and DDT at the sodium channel in rat dorsal root ganglion neurons

1996 ◽  
Vol 708 (1-2) ◽  
pp. 29-37 ◽  
Author(s):  
Jin-Ho Song ◽  
Keiichi Nagata ◽  
Hideharu Tatebayashi ◽  
Toshio Narahashi
Keyword(s):  
Dorsal Root Ganglion ◽  
Sodium Channel ◽  
Dorsal Root ◽  
Dorsal Root Ganglion Neurons ◽  
Ganglion Neurons

scholarly journals Differential Expression of Sodium Channel β Subunits in Dorsal Root Ganglion Sensory Neurons

2012 ◽  
Vol 287 (18) ◽  
pp. 15044-15053 ◽  
Author(s):  
Cojen Ho ◽  
Juan Zhao ◽  
Steven Malinowski ◽  
Mohamed Chahine ◽  
Michael E. O'Leary
Keyword(s):  
Dorsal Root Ganglion ◽  
Sodium Channel ◽  
Differential Expression ◽  
Sensory Neurons ◽  
Dorsal Root
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