scholarly journals Effects of cyclic dynamic tensile strain on previously compressed inner annulus fibrosus and nucleus pulposus cells of human intervertebral disc-an in vitro study

2009 ◽  
Vol 28 (4) ◽  
pp. 503-509 ◽  
Author(s):  
Hwan Tak Hee ◽  
Jituan Zhang ◽  
Hee Kit Wong
Keyword(s):  
Intervertebral Disc ◽  
Nucleus Pulposus ◽  
Annulus Fibrosus ◽  
Tensile Strain ◽  
In Vitro Study ◽  
Vitro Study ◽  
Nucleus Pulposus Cells ◽  
Cyclic Dynamic

In Vitro Study on Interaction Between Human Nucleus Pulposus Cells and Mesenchymal Stem Cells Through Paracrine Stimulation

Spine ◽  
2008 ◽  
Vol 33 (18) ◽  
pp. 1951-1957 ◽  
Author(s):  
Shu-Hua Yang ◽  
Chang-Chin Wu ◽  
Tiffany Ting-Fang Shih ◽  
Yuan-Hui Sun ◽  
Feng-Huei Lin
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Nucleus Pulposus ◽  
In Vitro Study ◽  
Vitro Study ◽  
Nucleus Pulposus Cells

In Vitro Study on the Mechanism of Autophagy of Nucleus Pulposus Cells Induced by Glycosylation of O-GlcNAc

2018 ◽  
Vol 8 (1) ◽  
pp. 20-26
Author(s):  
Xuan Wang ◽  
Yu-Chang Liu ◽  
Jiu-Hui Han ◽  
Jun-Zhong Luo ◽  
Ya-Zhou Li ◽  
...  
Keyword(s):  
Nucleus Pulposus ◽  
In Vitro Study ◽  
Vitro Study ◽  
Nucleus Pulposus Cells

scholarly journals Ligustrazine Prevents Intervertebral Disc Degeneration via Suppression of Aberrant TGFβ Activation in Nucleus Pulposus Cells

2019 ◽  
Vol 2019 ◽  
pp. 1-9
Author(s):  
Shufen Liu ◽  
Yuhao Cheng ◽  
Yuqi Tan ◽  
Jingcheng Dong ◽  
Qin Bian
Keyword(s):  
Growth Factor ◽  
Mouse Model ◽  
Intervertebral Disc ◽  
Nucleus Pulposus ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Ex Vivo ◽  
Tissue Growth ◽  
Nucleus Pulposus Cells

Objectives. Aberrant transforming growth factor β (TGFβ) activation is detrimental to both nucleus pulposus (NP) cells and cartilage endplates (CEPs), which can lead to intervertebral disc degeneration (IDD). Ligustrazine (LIG) reduces the expression of inflammatory factors and TGFβ1 in hypertrophic CEP to prevent IDD. In this study, we investigate the effects of LIG on NP cells and the TGFβ signaling. Design. LIG was injected to the lumbar spinal instability (LSI) mouse model. The effect of LIG was evaluated by intervertebral disc (IVD) score in the LSI mouse model. The expression of activated TGFβ was examined using immunostaining with pSmad2/3 antibody. The upright posture (UP) rat model was also treated and evaluated in the same manner to assess the effect of LIG. In ex vivo study, IVDs from four-week old mice were isolated and treated with 10−5, 10−6, and 10−7 M of LIG. We used western blot to detect activated TGFβ expression. TGFβ-treated human nucleus pulposus cells (HNPCs) were cotreated with optimized dose of LIG in vitro. Immunofluorescence staining was performed to determine pSmad2/3, connective tissue growth factor (CCN2), and aggrecan (ACAN) expression levels. Results. IVD score and the percentage of pSmad2/3+ NP cells were low in LIG-treated LSI mice in comparison with LSI mice, but close to the levels in the Sham group. Similarly, LIG reduced the overexpression of TGFβ1 in NP cells. The inhibitory effect of LIG was dose dependent. A dose of 10−5 M LIG not only strongly attenuated Smad2/3 phosphorylation in TGFβ-treated IVD ex vivo but also suppressed pSmad2/3, CCN2, and ACAN expression in TGFβ-treated NP cells in vitro. Conclusions. LIG prevents IDD via suppression of TGFβ overactivation in NP cells.

scholarly journals Testing the impact of discoplasty on the biomechanics of the intervertebral disc with simulated degeneration: An in vitro study

2020 ◽  
Vol 84 ◽  
pp. 51-59 ◽  
Author(s):  
Chloé Techens ◽  
Marco Palanca ◽  
Peter Endre Éltes ◽  
Áron Lazáry ◽  
Luca Cristofolini
Keyword(s):  
Intervertebral Disc ◽  
In Vitro Study ◽  
Vitro Study ◽  
The Impact

An In-vitro Study on Regeneration of Human Nucleus Pulposus by Using Gelatin/Chondroitin-6-Sulfate/Hyaluronan Tri-copolymer Scaffold

2005 ◽  
Vol 29 (10) ◽  
pp. 806-814 ◽  
Author(s):  
Shu-Hua Yang ◽  
Po-Quang Chen ◽  
Yi-Fang Chen ◽  
Feng-Huei Lin
Keyword(s):  
Nucleus Pulposus ◽  
In Vitro Study ◽  
Vitro Study
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