scholarly journals Histological and scanning electron microscopy analyses of bone/implant interface using the novel Bonelike® synthetic bone graft

2006 ◽  
Vol 24 (5) ◽  
pp. 953-958 ◽  
Author(s):  
M. Gutierres ◽  
N. Sooraj Hussain ◽  
M.A. Lopes ◽  
A. Afonso ◽  
A.T. Cabral ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Bone Graft ◽  
The Novel ◽  
Bone Implant ◽  
Synthetic Bone ◽  
Synthetic Bone Graft ◽  
Scanning Electron

Single-stage electrospun innovative combination of polyurethane and neem oil: Synthesis, characterization and appraisal of blood compatibility

2018 ◽  
Vol 33 (6) ◽  
pp. 573-584 ◽  
Author(s):  
Mohan Prasath Mani ◽  
Saravana Kumar Jaganathan ◽  
Ahmad Zahran Khudzari ◽  
Rajasekar Rathanasamy ◽  
Praseetha Prabhakaran
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Contact Angle ◽  
Partial Thromboplastin Time ◽  
Blood Compatibility ◽  
Activated Partial Thromboplastin Time ◽  
The Novel ◽  
Neem Oil ◽  
Composite Patch ◽  
Scanning Electron

Wound healing is a complex process and it requires proper scaffolding for regeneration. An ideal scaffold should provide optimal environmental conditions in order to assist cellular attachment, proliferation and differentiation. In this work, a new composite based on polyurethane and neem oil was fabricated using one-step electrospinning technique. Fabricated composite patch along with the pristine polyurethane was characterized through scanning electron microscopy, Fourier transform and infrared spectroscopy, thermogravimetric analysis, contact angle measurement and atomic force microscopy. Moreover, the blood compatibility was evaluated using activated partial thromboplastin time, partial thromboplastin time and haemolysis assay. Scanning electron microscopy studies of composites revealed the existence of fibres with a smaller diameter (635  ± 105 nm) compared to the pristine polyurethane (969 ± 217 nm). Fourier transform and infrared analysis revealed the formation of hydrogen bond and peak shifting characteristics confirming the interaction of the neem oil with the polyurethane. Contact angle analysis showed the decrease in contact angle indicating the hydrophilic nature of the fabricated patch compared to pristine polyurethane. Thermal gravimetric analysis depicted the better thermal stability of the novel composite patch due to the existence of neem oil in the pristine polyurethane. The presence of neem oil in polyurethane matrix also resulted in an increase in the surface roughness as observed in the AFM analysis. The novel composite patch showed an ability to reduce the thrombogenicity and promoting the anticoagulant nature signified by blood compatibility assays like activated partial thromboplastin time and partial thromboplastin time. Finally, the haemolytic percentage of the fabricated composite (1%) was found to be reduced compared to control (2.733%) indicating better blood compatibility and safety with the red blood cells. Following the results, the fabricated patches offered enhanced physicochemical and blood compatible nature making it as a promising candidate for wound healing application.

scholarly journals The Removal of HBV in Plasma by Extracorporeal Immunoadsorption from Plasma: A Potential Therapy of Hepatitis B Patients

2018 ◽  
Vol 2018 ◽  
pp. 1-7
Author(s):  
Zhenwei Han ◽  
Xuan Lu ◽  
Yiping Tang ◽  
Yuanyuan Yang ◽  
Qiuchen Liu ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Monoclonal Antibody ◽  
Time Dependent ◽  
The Novel ◽  
Plasma Samples ◽  
Novel Therapy ◽  
Real Time Quantitative Pcr ◽  
Therapy Option ◽  
Scanning Electron

Objective. To establish a novel HBV specific immunoadsorbent for the removing of HBV particles.Methods. The anti-HBsAg monoclonal antibody was immobilized on sepharose beads to produce a sepharose anti-HBs column. Then the immunoadsorbent was evaluated and characterized by scanning electron microscopy. In addition, time-dependent effects of the eradication capacity of anti-HBsAg functionalized sepharose beads against HBV were investigated.Results. Proposed immunoadsorbents exhibited a favorable biocompatibility as well as specificity. With the optimized recycle time, the decontamination performance of HBV particles and quantity of HBsAg were assessed either by real-time quantitative PCR or ELISA, which showed that the immunoadsorbent could remove approximately 90% of the HBV and 90% of the HBsAg from human plasma samples.Conclusions. All these results indicated that the novel immunoadsorbent could effectively remove HBV particles and likely serve as a novel therapy option or at least supplementary for the treatment regimen of HBV.

scholarly journals SARS-CoV-2: Enhancement and Segmentation of High-ResolutionMicroscopy Images. Part I

2020 ◽  
Author(s):  
Roberto Rodriguez ◽  
Brian A. Mondeja ◽  
Odalys Valdes ◽  
Sonia Resik ◽  
Ananayla Vizcaino ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Eating Habits ◽  
World Population ◽  
The Novel ◽  
Rt Pcr ◽  
Unhealthy Lifestyle ◽  
Microscopic Images ◽  
Novel Coronavirus ◽  
Scanning Electron

Abstract Possibly, and due to poor eating habits and unhealthy lifestyle, many virus are transmitted to human people. Such is the case, of the novel coronavirus SARS-Cov-2, which has expanded of exponential way, practically, to whole world population. For this reason, the study of real microscopic images of this coronavirus is of great importance. The SARS-Cov-2 images were captured from nasopharyngeal samples of Cuban symptomatic individuals (RT-PCR positives for SARS-CoV-2), and processed via scanning electron microscopy. However, many times these microscopic images present some blurring problems, which are always susceptible to be improved. The aim of this work is to propose new computational methods to carry out enhancement and segmentation of SARS-Cov-2 high-resolution microscopic images. Moreover, due to the importance of the obtained results, this first work will be addressed to the application of the proposed algorithm. The proposed strategy obtained very satisfactory results, and we validated its performance, together with specialist physicians, on a set of 1005 images. A second paper will deeply analyze the theory related to these algorithms

Tribological Performance of 3-(N-mono-n-lurylaminomethyl) quinazolin-4-one as Additive in Liquid Paraffin

2011 ◽  
Vol 71-78 ◽  
pp. 1049-1052
Author(s):  
Ping Ouyang ◽  
Xian Ming Zhang
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Photoelectron Spectroscopy ◽  
Tribological Behavior ◽  
Liquid Paraffin ◽  
Tribological Performance ◽  
The Novel ◽  
Heterocyclic Derivative ◽  
X Ray ◽  
Scanning Electron

A heterocyclic derivative of 3-(N-mono-n-lurylaminomethyl) quinazolin-4-one was synthesized and its tribological behavior as an ashless additve without phosphorus and sulphur in liquid paraffin was evaluated using a four-ball tester. On the basis of the experimental results, the novel additive has been found to be quite effective as a potential additive in liquid paraffin. The nature of the film on the rubbed surface was investigated by scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS).

The Performance and Antiwear Mechanism of 3-(N-mono-n- butylaminomethyl) Quinazolin-4-One as Additive in Liquid Paraffin

2011 ◽  
Vol 80-81 ◽  
pp. 294-297
Author(s):  
Ou Yang Ping ◽  
Xian Ming Zhang
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Photoelectron Spectroscopy ◽  
Tribological Behavior ◽  
Liquid Paraffin ◽  
Experimental Results ◽  
The Novel ◽  
Heterocyclic Derivative ◽  
X Ray ◽  
Scanning Electron

A heterocyclic derivative of 3-(N-mono-n-butylaminomethyl) quinazolin-4-one was synthesized and its tribological behavior as an ashless additve without phosphorus and sulphur in liquid paraffin was evaluated using a four-ball tester. On the basis of the experimental results, the novel additive has been found to be quite effective as a potential additive in liquid paraffin. The nature of the film on the rubbed surface was investigated by scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS).

scholarly journals Characterizing the Novel Fruit Surface Morphology of `Marina' Peach Using Scanning Electron Microscopy

1996 ◽  
Vol 121 (2) ◽  
pp. 198-203
Author(s):  
Michael A. Creller ◽  
Dennis J. Werner
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Surface Morphology ◽  
Plant Introduction ◽  
The Novel ◽  
Trichome Density ◽  
Flower Bud ◽  
Delayed Initiation ◽  
Fruit Surface ◽  
Scanning Electron

Scanning electron microscopy (SEM) was used to compare the novel surface morphology of `Marina' peach [plant introduction (PI) 133984] to a normal peach (`Contender') and a nectarine (`Sunglo'). Samples were collected before, during, and after anthesis. Compared to `Contender', `Marina' showed different trichome structure, lower trichome density, and delayed initiation of trichomes on the gynoecium. No pubescence was observed on `Sunglo' nectarine at any sampling date. Trichomes were present on the flower bud scales of all three cultivars. Arrangement and structure of trichomes on flower bud scales of `Marina' differed from those on `Contender' and `Sunglo'.

scholarly journals SARS-CoV-2: High-Resolution Microscopy Study in Human Nasopharyngeal Samples.

2020 ◽  
Author(s):  
Brian Mondeja ◽  
Odalys Valdes ◽  
Sonia Resik ◽  
Ananayla Vizcaino ◽  
Emilio Acosta ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
High Resolution ◽  
Microscopy Study ◽  
Negative Control ◽  
The Novel ◽  
Rt Pcr ◽  
Novel Coronavirus ◽  
High Resolution Microscopy ◽  
Scanning Electron

Abstract BackgroundThe novel coronavirus SARS-CoV-2 is the etiological agent of COVID-19. This virus has become one of the most dangerous in recent times with a very high rate of transmission. At present, several publications show the typical crown-shape of the novel coronavirus grown in cell cultures. However, an integral ultramicroscopy study done directly from clinical specimens has not been published. Methods Nasopharyngeal swabs were collected from two Cuban individuals, one asymptomatic and RT-PCR negative (negative control) and the other from a COVID-19 symptomatic and RT-PCR positive for SARS CoV-2. Samples were treated with an aldehyde solution and processed by Scanning Electron Microscopy, Confocal Microscopy and, Atomic Force Microscopy. Improvement and segmentation of coronavirus images were performed by mathematic algorithms. Results The images of the negative control sample showed the characteristic healthy microvilli morphology at the apical region of the nasal epithelial cells. As expected, they do not display virus-like structures. The images of the positive sample showed characteristic coronavirus-like particles and evident destruction of microvilli. In some regions, virions gemmating through the cell membrane were observed. Microvilli destruction could explain the anosmia reported by some patients. Virus-particles emerging from the cell-surface with a variable size ranging from 80 to 400 nm were observed by scanning electron microscopy (SEM). A confocal study showed viral antigen recognition in the apical cells zone. Conclusions The integral microscopy study showed that SARS-CoV-2 has a similar image to SARS-CoV. The application of several high-resolution microscopy techniques to clinical samples can help to answer important questions its replicative cycle and immunopathogenic mechanism of this novel coronavirus, relevant for the development of new treatments against this disease.

scholarly journals SARS-CoV-2: High-resolution microscopy study in human nasopharyngeal samples

2020 ◽  
Author(s):  
Brian Mondeja ◽  
Odalys Valdes ◽  
Sonia Resik ◽  
Ananayla Vizcaino ◽  
Emilio Acosta ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
High Resolution ◽  
Microscopy Study ◽  
Negative Control ◽  
The Novel ◽  
Rt Pcr ◽  
Novel Coronavirus ◽  
High Resolution Microscopy ◽  
Scanning Electron

Abstract Background The novel coronavirus SARS-CoV-2 is the etiological agent of COVID-19. This virus has become one of the most dangerous in recent times with a very high rate of transmission. At present, several publications show the typical crown-shape of the novel coronavirus grown in cell cultures. However, an integral ultramicroscopy study done directly from clinical specimens has not been published. Methods Nasopharyngeal swabs were collected from two Cuban individuals, one asymptomatic and RT-PCR negative (negative control) and the other from a COVID-19 symptomatic and RT-PCR positive for SARS CoV-2. Samples were treated with an aldehyde solution and processed by Scanning Electron Microscopy (SEM), Confocal Microscopy (CM) and, Atomic Force Microscopy (AFM). Improvement and segmentation of coronavirus images were performed by a novel mathematical image enhancement algorithm. Results The images of the negative control sample showed the characteristic healthy microvilli morphology at the apical region of the nasal epithelial cells. As expected, they do not display virus-like structures. The images of the positive sample showed characteristic coronavirus-like particles and evident destruction of microvilli. In some regions, virions budding through the cell membrane were observed. Microvilli destruction could explain the anosmia reported by some patients. Virus-particles emerging from the cell-surface with a variable size ranging from 80 to 400 nm were observed by scanning electron microscopy (SEM). Viral antigen was identified in the apical cells zone by CM. Conclusions The integral microscopy study showed that SARS-CoV-2 has a similar image to SARS-CoV. The application of several high-resolution microscopy techniques to nasopharyngeal samples awaits future use.

Effect of Ultrasonic Vibration on Electrospun Poly(vinyl Alcohol) (PVA) Nanofibers

2013 ◽  
Vol 843 ◽  
pp. 1-8 ◽  
Author(s):  
Na Si ◽  
Lan Xu ◽  
Mei Zhen Wang ◽  
Fu Juan Liu
Keyword(s):  
Electron Microscopy ◽  
Electrical Conductivity ◽  
Scanning Electron Microscopy ◽  
Ultrasonic Vibration ◽  
Vinyl Alcohol ◽  
Poly Vinyl Alcohol ◽  
The Novel ◽  
Novel Strategy ◽  
Radiation Time ◽  
Scanning Electron

Poly (vinyl alcohol) (PVA), a classical biodegradable polymer, is successfully fabricated into nanofibers via the vibration-electrospinning, and the obtained nanofibers are characterized by the scanning electron microscopy (SEM). The viscosity and electrical conductivity of PVA solution vary dramatically with the ultrasonic radiation time and intensity. The novel strategy can produce finer nanofibers than those obtained without ultrasonic vibration.

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