scholarly journals Nonadherent cell population of human marrow culture is a complementary source of mesenchymal stem cells (MSCs)

2005 ◽  
Vol 24 (1) ◽  
pp. 21-28 ◽  
Author(s):  
Chao Wan ◽  
Qiling He ◽  
Mervyn McCaigue ◽  
David Marsh ◽  
Gang Li
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Population ◽  
Nonadherent Cell

scholarly journals Mesenchymal stem cells generate a CD4+CD25+Foxp3+ regulatory T cell population during the differentiation process of Th1 and Th17 cells

2013 ◽  
Vol 4 (3) ◽  
pp. 65 ◽  
Author(s):  
Patricia Luz-Crawford ◽  
Monica Kurte ◽  
Javiera Bravo-Alegría ◽  
Rafael Contreras ◽  
Estefania Nova-Lamperti ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
T Cell ◽  
Cell Population ◽  
Regulatory T Cell ◽  
Th17 Cells ◽  
Differentiation Process

scholarly journals SSEA-4 identifies mesenchymal stem cells from bone marrow

Blood ◽  
2006 ◽  
Vol 109 (4) ◽  
pp. 1743-1751 ◽  
Author(s):  
Eun J. Gang ◽  
Darko Bosnakovski ◽  
Camila A. Figueiredo ◽  
Jan W. Visser ◽  
Rita C. R. Perlingeiro
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cell Population ◽  
Embryonic Stem ◽  
Blastocyst Stage ◽  
Research Progress ◽  
Stem Cell Population ◽  
Hematopoietic Stem ◽  
Suitable Marker

Abstract Adult bone marrow (BM) contains hematopoietic stem cells (HSCs) as well as a nonhematopoietic, stromal cell population. Within this stromal population are mesenchymal stem cells (MSCs), which not only support hematopoiesis but also differentiate into multiple lineages, including fat, bone, and cartilage. Because of this multipotentiality, the MSC is an attractive candidate for clinical applications to repair or regenerate damaged tissues of mesenchymal origin. However, research progress has been hampered by the limited existing knowledge of the biology of these cells, particularly by the lack of a suitable marker for their prospective isolation. Here, we report that SSEA-4, an early embryonic glycolipid antigen commonly used as a marker for undifferentiated pluripotent human embryonic stem cells and cleavage to blastocyst stage embryos, also identifies the adult mesenchymal stem cell population.

scholarly journals Derivation of Neural Stem Cells from Mesenchymal Stem Cells: Evidence for a Bipotential Stem Cell Population

2008 ◽  
Vol 17 (6) ◽  
pp. 1109-1122 ◽  
Author(s):  
Lijuan Fu ◽  
Lunjian Zhu ◽  
Yu Huang ◽  
Tsung D. Lee ◽  
Stephen J. Forman ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Neural Stem Cells ◽  
Cell Population ◽  
Stem Cell Population

Both Expanded and Uncultured Mesenchymal Stem Cells from MDS Patients Are Genomically Abnormal, Showing a Specific Genetic Profile for the 5q- Syndrome.

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 1650-1650
Author(s):  
Olga López Villar ◽  
Juan LuíS. García ◽  
Fermín. M Sánchez Guijo ◽  
Cristina Robledo ◽  
Pilar Hernandez Campo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Population ◽  
Array Cgh ◽  
Genomic Alteration ◽  
Comparative Genomic ◽  
Genetic Profile ◽  
Unsupervised Hierarchical Cluster ◽  
Genomic Changes ◽  
Genomic Aberrations

Abstract In a previous report (López et al, ASH 2007, abstract 1916) we have demonstrated the presence of cytogenetic aberrations on mesenchymal stem cells (MSCs), the bone marrow (BM) stroma progenitors, from patients with myelodysplastic syndromes (MDS). Since there is a chance that the observed changes could be the result of the expansion in the culture process the aim of the present study was to analyze whether genomic changes could be present in non-expanded BM MSC and also to assess if the genomic changes detected could be correlated with specific subtypes of MDS. To address our first issue, in four MDS cases, MSCs were enriched by sorting mononuclear cells from BM with the following phenotype: CD45−/CD73++/CD34−/CD271+++, purity being >99%. Array based comparative genomic hybridization (array-CGH) was performed with this cell population. DNA was extracted, and after it was amplificated using the GenomiPhi kit. The postamplification cleanup was achived by ethanol precipitation using the sodiumacetate/EDTA. In order to know whether the amplified products contained actual genomics a PCR, using specific primers was performed; both test and reference DNA were amplified using the same amount of starting DNA. With this approach genomic changes were observed in this sorted cell population, with gains being more frequent than loses. The more frequently affected genomic regions were: 1q31, 10q26 and 20q13. In order to be sure that these features were also present in expanded MSCs, FISH using the same bacterial artificial chromosome (BAC) probe than used in the array-CGH studies was performed on expanded MSCs from the same patients and the same genomic alteration was observed, showing that not only expanded but also freshly isolated MSCs have genomic aberrations. Once demostrated that. uncultured MSCs were genomically abnormal we wanted to know whether these aberrations could be related to any clinical parameter. For this purpose expanded MSCs from 13 MDS patients were studied using array-CGH and an unsupervised hierarchical cluster analysis performed. Two different clusters were identified: one of them included all the 5q- syndrome patients, while the other incorporated the remaining MDS subtypes. The differences between these two clusters occurred just in 26 BAC. When looking at these clones and taking into account only regions involving at least two consecutive BACS, chromosomal regions 11q13, 17q25, 19q13, 20q13, and 22q13.2 were gained in 100% or 80% of the 5q-cases; by contrast a normal copy or a deletion of these regions were observed within the rest of patients. Our results show, for the first time, that both cultured-expanded and freshly-isolated MSC from MDS display genomic aberrations, as assessed by array-CGH and FISH, some of them specially linked to a specific clinical entity, the 5q- syndrome.

scholarly journals A Transformed Cell Population Derived from Cultured Mesenchymal Stem Cells has no Functional Effect after Transplantation into the Injured Heart

2009 ◽  
Vol 18 (3) ◽  
pp. 319-332 ◽  
Author(s):  
Dario Furlani ◽  
Wenzhong Li ◽  
Erik Pittermann ◽  
Christian Klopsch ◽  
Liang Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Population ◽  
Functional Effect ◽  
Transformed Cell

Ear mesenchymal stem cells: An efficient adult multipotent cell population fit for rapid and scalable expansion

2009 ◽  
Vol 139 (4) ◽  
pp. 291-299 ◽  
Author(s):  
Sébastien Sart ◽  
Yves-Jacques Schneider ◽  
Spiros N. Agathos
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Population
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