Exposure of mouse preosteoblasts to pulsed electromagnetic fields reduces the amount of mature, type I collagen in the extracellular matrix

2006 ◽  
Vol 24 (2) ◽  
pp. 242-253 ◽  
Author(s):  
Yoshitada Sakai ◽  
Thomas E. Patterson ◽  
Michael O. Ibiwoye ◽  
Ronald J. Midura ◽  
Maciej Zborowski ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Electromagnetic Fields ◽  
Type I Collagen ◽  
Type I ◽  
Pulsed Electromagnetic Fields ◽  
Mature Type ◽  
Pulsed Electromagnetic

scholarly journals Pulsed electromagnetic fields improve the healing process of Achilles tendinopathy

2020 ◽  
Vol 9 (9) ◽  
pp. 613-622
Author(s):  
Carlotta Perucca Orfei ◽  
Arianna Barbara Lovati ◽  
Gaia Lugano ◽  
Marco Viganò ◽  
Marta Bottagisio ◽  
...  
Keyword(s):  
Electromagnetic Fields ◽  
Acute Phase ◽  
Healing Process ◽  
Tendon Healing ◽  
Achilles Tendinopathy ◽  
Type I ◽  
Pulsed Electromagnetic Fields ◽  
Sprague Dawley ◽  
Positive Role ◽  
Pulsed Electromagnetic

Aims In the context of tendon degenerative disorders, the need for innovative conservative treatments that can improve the intrinsic healing potential of tendon tissue is progressively increasing. In this study, the role of pulsed electromagnetic fields (PEMFs) in improving the tendon healing process was evaluated in a rat model of collagenase-induced Achilles tendinopathy. Methods A total of 68 Sprague Dawley rats received a single injection of type I collagenase in Achilles tendons to induce the tendinopathy and then were daily exposed to PEMFs (1.5 mT and 75 Hz) for up to 14 days - starting 1, 7, or 15 days after the injection - to identify the best treatment option with respect to the phase of the disease. Then, 7 and 14 days of PEMF exposure were compared to identify the most effective protocol. Results The daily exposure to PEMFs generally provided an improvement in the fibre organization, a decrease in cell density, vascularity, and fat deposition, and a restoration of the physiological cell morphology compared to untreated tendons. These improvements were more evident when the tendons were exposed to PEMFs during the mid-acute phase of the pathology (7 days after induction) rather than during the early (1 day after induction) or the late acute phase (15 days after induction). Moreover, the exposure to PEMFs for 14 days during the mid-acute phase was more effective than for 7 days. Conclusion PEMFs exerted a positive role in the tendon healing process, thus representing a promising conservative treatment for tendinopathy, although further investigations regarding the clinical evaluation are needed. Cite this article: Bone Joint Res 2020;9(9):613–622.

scholarly journals Pulsed Electromagnetic Fields Improve Tenogenic Commitment of Umbilical Cord-Derived Mesenchymal Stem Cells: A Potential Strategy for Tendon Repair—An In Vitro Study

2018 ◽  
Vol 2018 ◽  
pp. 1-18 ◽  
Author(s):  
Antonio Marmotti ◽  
Giuseppe Maria Peretti ◽  
Silvia Mattia ◽  
Laura Mangiavini ◽  
Laura de Girolamo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Electromagnetic Fields ◽  
Umbilical Cord ◽  
Tendon Repair ◽  
Human Umbilical Cord ◽  
Type I ◽  
Pulsed Electromagnetic Fields ◽  
Pulsed Electromagnetic

Tendon repair is a challenging procedure in orthopaedics. The use of mesenchymal stem cells (MSCs) and pulsed electromagnetic fields (PEMF) in tendon regeneration is still investigational. In this perspective, MSCs isolated from the human umbilical cord (UC) may represent a possible candidate for tendon tissue engineering. The aim of the study is to evaluate the effect of low-frequency PEMF on tenogenic differentiation of MSCs isolated from the human umbilical cord (UC-MSCs) in vitro. 15 fresh UC samples from women with healthy pregnancies were retrieved at the end of caesarean deliveries. UC samples were manually minced into small fragments (less than 4 mm length) and cultured in MSC expansion medium. Part of the UC-MSCs was subsequently cultured with PEMF and tenogenic growth factors. UC-MSCs were subjected to pulsed electromagnetic fields for 2 h/day, 4 h/day, or 8 h/day. UC-MSCs cultured with FGF-2 and stimulated with PEMF showed a greater production of collagen type I and scleraxis. The prolonged exposure to PEMF was also related to the greatest expression of tenogenic markers. Thus, the exposure to PEMF provides a positive preconditioning biophysical stimulus, which may enhance UC-MSC tenogenic potential.

Effect of pulsed electromagnetic fields on maturation of regenerate bone in a rabbit limb lengthening model

2005 ◽  
Vol 24 (1) ◽  
pp. 2-10 ◽  
Author(s):  
Kenneth F. Taylor ◽  
Nozumu Inoue ◽  
Bahman Rafiee ◽  
John E. Tis ◽  
Kathleen A. McHale ◽  
...  
Keyword(s):  
Electromagnetic Fields ◽  
Limb Lengthening ◽  
Pulsed Electromagnetic Fields ◽  
Pulsed Electromagnetic

Effects of Pulsed Electromagnetic Fields on Rat Eye

1999 ◽  
Vol 18 (2) ◽  
pp. 157-164
Author(s):  
Gianluca Giavaresi ◽  
Francesco Broccoli ◽  
Milena Fini ◽  
Paola Torricelli ◽  
Paola Versura ◽  
...  
Keyword(s):  
Electromagnetic Fields ◽  
Pulsed Electromagnetic Fields ◽  
Pulsed Electromagnetic

scholarly journals The Dynamic Interaction between Extracellular Matrix Remodeling and Breast Tumor Progression

Cells ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1046
Author(s):  
Jorge Martinez ◽  
Patricio C. Smith
Keyword(s):  
Extracellular Matrix ◽  
Type I Collagen ◽  
Cell Metabolism ◽  
Breast Tumors ◽  
Extracellular Matrix Remodeling ◽  
Type I ◽  
Matrix Remodeling ◽  
Desmoplastic Reaction ◽  
The Impact

Desmoplastic tumors correspond to a unique tissue structure characterized by the abnormal deposition of extracellular matrix. Breast tumors are a typical example of this type of lesion, a property that allows its palpation and early detection. Fibrillar type I collagen is a major component of tumor desmoplasia and its accumulation is causally linked to tumor cell survival and metastasis. For many years, the desmoplastic phenomenon was considered to be a reaction and response of the host tissue against tumor cells and, accordingly, designated as “desmoplastic reaction”. This notion has been challenged in the last decades when desmoplastic tissue was detected in breast tissue in the absence of tumor. This finding suggests that desmoplasia is a preexisting condition that stimulates the development of a malignant phenotype. With this perspective, in the present review, we analyze the role of extracellular matrix remodeling in the development of the desmoplastic response. Importantly, during the discussion, we also analyze the impact of obesity and cell metabolism as critical drivers of tissue remodeling during the development of desmoplasia. New knowledge derived from the dynamic remodeling of the extracellular matrix may lead to novel targets of interest for early diagnosis or therapy in the context of breast tumors.

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