Effects of immobilization on achilles tendon healing in a rat model

1994 ◽  
Vol 12 (4) ◽  
pp. 582-591 ◽  
Author(s):  
George A. C. Murrell ◽  
Edward G. Lilly ◽  
Richard D. Goldner ◽  
Anthony V. Seaber ◽  
Thomas M. Best
Keyword(s):  
Achilles Tendon ◽  
Rat Model ◽  
Tendon Healing

scholarly journals Phenytoin accelerates tendon healing in a rat model of Achilles tendon rupture

2016 ◽  
Vol 117 (09) ◽  
pp. 543-546
Author(s):  
B. Hajipour ◽  
A. M. Navali ◽  
S. Ali Mohammad ◽  
G. Mousavi ◽  
M. Gahvechi Akbari ◽  
...  
Keyword(s):  
Achilles Tendon ◽  
Rat Model ◽  
Tendon Rupture ◽  
Achilles Tendon Rupture ◽  
Tendon Healing

scholarly journals Effect of recombinant human platelet-derived growth factor-BB-coated sutures on Achilles tendon healing in a rat model: A histological and biomechanical study

2012 ◽  
Vol 3 (1) ◽  
pp. 204173141245357 ◽  
Author(s):  
Stephen H Cummings ◽  
Daniel A Grande ◽  
Christopher K Hee ◽  
Hans K Kestler ◽  
Colleen M Roden ◽  
...  
Keyword(s):  
Growth Factor ◽  
Achilles Tendon ◽  
Rat Model ◽  
Human Platelet ◽  
Tendon Healing ◽  
Biomechanical Study ◽  
Platelet Derived Growth Factor

scholarly journals Liquid Poly-N-acetyl Glucosamine (sNAG) Improves Achilles Tendon Healing in a Rat Model

2021 ◽  
Author(s):  
Courtney A. Nuss ◽  
Julianne Huegel ◽  
Sergio Finkielsztein ◽  
Louis J. Soslowsky
Keyword(s):  
Achilles Tendon ◽  
Rat Model ◽  
Tendon Healing

scholarly journals Ex vivo adenoviral transfer of bone morphogenetic protein 12 (BMP-12) cDNA improves Achilles tendon healing in a rat model

Gene Therapy ◽  
2008 ◽  
Vol 15 (16) ◽  
pp. 1139-1146 ◽  
Author(s):  
M Majewski ◽  
O Betz ◽  
P E Ochsner ◽  
F Liu ◽  
R M Porter ◽  
...  
Keyword(s):  
Achilles Tendon ◽  
Bone Morphogenetic Protein ◽  
Rat Model ◽  
Ex Vivo ◽  
Tendon Healing ◽  
Morphogenetic Protein ◽  
Adenoviral Transfer

scholarly journals Comparison of Achilles Tendon Healing After Exposure to Combusted Tobacco, Vaping, and Control in a Rat Model

2019 ◽  
Vol 7 (7_suppl5) ◽  
pp. 2325967119S0032
Author(s):  
Patrick Kennedy ◽  
Kaitlin Saloky ◽  
Aditya Yadavalli ◽  
Erin Barlow ◽  
Michael Aynardi ◽  
...  
Keyword(s):  
Tensile Strength ◽  
Achilles Tendon ◽  
Rat Model ◽  
Tendon Healing ◽  
Maximum Force ◽  
Control Group ◽  
Nicotine Exposure ◽  
Sprague Dawley ◽  
Orthopedic Injury ◽  
Load To Failure

Objectives: The negative effects of smoking have been well documented following orthopedic injury. Though nicotine has been shown to be detrimental to musculoskeletal tissue healing, nicotine in the form of “Vaping” is being increasingly used as a perceived healthier alternative to actual smoking. It may theoretically obviate many of the harmful volatiles and chemicals contained within combusted tobacco that are additionally harmful to musculoskeletal healing, beyond just nicotine. The literature has yet to establish the benefits, or lack thereof, to exposure of e-cigarettes and their effect on tendon healing when compared to traditional combusted tobacco. Our objective was to evaluate the biomechanical and histological effects on Achilles tendon repair between inhaled combusted tobacco versus isolated nicotine exposure via vaping versus a control group in a small animal (Sprague Dawley Rat) model. Methods: 54 Sprague Dawley rats were randomly placed in a control, vaping, or cigarette cohort. Each group contained 17 rats for exposure and they were exposed six days per week. The smoking cohort was exposed to 2 unfiltered University of Kentucky research cigarettes in a smoking chamber (Image 1). The vaping group was exposed to e-cigarette vapor with equivalent nicotine exposure as compared to the cigarette group, over ten minutes at a flow rate of 2.4 L/min. The control group was placed in the smoking chamber with room air flowing through the chamber. All rats received their respective daily exposures for 4 weeks prior to surgery where transection and repair of the Achilles tendon was performed. Following surgery, the rats received 2 additional weeks of smoking vs vaping vs control exposure. After sacrifice, Achilles tendons were harvested and tested with tensile and a load to failure model (Image 2). Histological samples were sent for analysis. Results: Tensile load testing evaluated maximum force to rupture and tissue stiffness amongst the three cohorts. The control group demonstrated highest mean tensile strength of 41.0 N (18.3-55.1 N), with the cigarette cohort having the second highest mean tensile strength at 37.3 N (14.0-54.7 N), and finally the vaping group had the lowest at 32.28 N (17.8-45.1 N). One-way ANOVA with heterogeneous of variance was used for evaluation. There was a significant difference noted in load to failure when comparing controls to e-cigarettes (p=0.026). No statistical difference was seen between controls vs cigarettes (p=0.35). Histological analysis is in progress. Conclusion: Our investigation demonstrates that in a rat model, isolated nicotine exposure via ”vaping” significantly impedes biomechanical healing properties of Achilles tendon surgical repair. Though smoking resulted in a lower maximum force to failure as compared to control, this difference was not significant. While E-cigarettes are often utilized as a perceived “safer” alternative to smoking combusted tobacco, this study suggests that use of e-cigarettes may be more detrimental to tendon healing than combusted tobacco in a rat Achilles model.

Effects of Exercise on Achilles Tendon Healing in a Rat Model

1998 ◽  
Vol 19 (9) ◽  
pp. 598-603 ◽  
Author(s):  
George A.C. Murrell ◽  
Daniel Jang ◽  
Xiang-Hua Deng ◽  
Jo A. Hannafin ◽  
Russell F. Warren
Keyword(s):  
Achilles Tendon ◽  
Internal Control ◽  
Rat Model ◽  
Detrimental Effect ◽  
Functional Performance ◽  
Tendon Healing ◽  
Exercise Group ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
The Right

The effects of motion, or lack of it, on Achilles tendon healing are not well defined. We have recently shown that immobilization has a detrimental effect on tendon healing in a rat model. The aim of this experiment was to determine whether enforced exercise had an additional beneficial effect on the mechanical and functional recovery of divided Achilles tendons in rats. Male Sprague-Dawley rats were randomly allocated into a nonexercise and an exercise group (N = 10 for each group). In both groups the right Achilles tendon was surgically transected. The left, uninjured lower limb served as an internal control. Both groups of animals were housed under identical conditions with the exception that the exercise group swam for 15 minutes per day. Functional performance was determined from the measurement of hindpaw prints of walking rats preoperatively and on alternate postoperative days. On day 15, the animals were killed and weighed, and biomechanical evaluations were performed on both the injured and uninjured Achilles tendon constructs. There were no differences in weight at time of death. All animals had an initial functional deficit that returned to near-normal by day 15. There were significant differences in the morphological and the mechanical properties of the healing Achilles tendon constructs at day 15 when comparing the injured with the uninjured Achilles tendon constructs. Supplemental exercise, however, had no effect on the functional or mechanical recovery of injured or uninjured Achilles tendons in the rat model.

scholarly journals Vaporized vs Combusted Nicotine: A Biomechanical Comparison of Achilles Tendon Healing in a Rat Model

2019 ◽  
Vol 4 (4) ◽  
pp. 2473011419S0024
Author(s):  
Patrick Kennedy ◽  
Kaitlin Saloky ◽  
Aditya Yadavalli ◽  
Erin Barlow ◽  
Michael Aynardi ◽  
...  
Keyword(s):  
Tensile Strength ◽  
Achilles Tendon ◽  
Rat Model ◽  
Tendon Healing ◽  
Tensile Load ◽  
Maximum Force ◽  
Control Group ◽  
Nicotine Exposure ◽  
Orthopedic Injury ◽  
Significant Difference

Category: Basic Sciences/Biologics Introduction/Purpose: The negative effects of smoking have been well documented following orthopedic injury. Though nicotine has been shown to be detrimental to musculoskeletal tissue healing, nicotine in the form of “Vaping” is being increasingly used as a perceived healthier alternative to actual smoking and may theoretically obviate many of the harmful volatiles and chemicals contained within combusted tobacco that are additionally harmful to musculoskeletal healing. Our objective was to evaluate the biomechanical and histological effects on Achilles tendon repair between rats that inhaled combusted tobacco, versus those with isolated nicotine exposure via vaping, versus a control group. Methods: 54 Sprague Dawley rats were randomly placed in a control, vaping, or cigarette cohort. Each group contained 17 rats and exposure occurred six days per week. The smoking cohort was exposed to 2 unfiltered University of Kentucky research cigarettes in a previously validated smoking chamber (Figure 1). The vaping group was exposed to e-cigarette vapor with equivalent nicotine exposure over ten minutes at a flow rate of 2.4 L/min. The control group was placed in the smoking chamber with room air flowing through the chamber. All rats received their respective daily exposures for 4 weeks prior to surgery when surgical transection and repair of the Achilles tendon was performed. Following surgery, the rats continued 2 additional weeks of smoking, vaping, or control exposure. After sacrifice, Achilles tendons were harvested and tested with controlled tension to failure. Histology (n = 2 per group) was performed with a standard H&E protocol. Results: Tensile load testing evaluated maximum force to rupture and tissue stiffness amongst the three cohorts. The control group demonstrated highest mean tensile strength of 41.0 N (18.3-55.1 N), with the cigarette cohort having the second highest mean tensile strength at 37.3 N (14.0-54.7 N), and finally the vaping group had the lowest at 32.28 8 N (17.8-45.1 N). One-way ANOVA with heterogeneity of variance was used for evaluation. There was a significant difference detected in load to failure when comparing controls to e-cigarettes (p=0.026). No statistical difference was seen between controls vs cigarettes (p=0.35). Histological analysis demonstrated no difference among groups. Conclusion: Our investigation demonstrates that, in a rat model, isolated nicotine exposure via ”vaping” significantly impedes biomechanical healing properties of Achilles tendon surgical repair. Though smoking resulted in a lower maximum force to failure as compared to control in these samples, this difference was not significant. While E-cigarettes are often utilized as a perceived “safer” alternative to smoking combusted tobacco, this study suggests that use of e-cigarettes may be more detrimental to tendon healing than combusted tobacco in a rat Achilles model.

Effect of curcumin on tendon healing: an experimental study in a rat model of Achilles tendon injury

2018 ◽  
Vol 42 (8) ◽  
pp. 1905-1910 ◽  
Author(s):  
Ali Güleç ◽  
Yılmaz Türk ◽  
Bahattin Kerem Aydin ◽  
Ömer Faruk Erkoçak ◽  
Selim Safalı ◽  
...  
Keyword(s):  
Experimental Study ◽  
Achilles Tendon ◽  
Rat Model ◽  
Tendon Healing ◽  
Tendon Injury
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