Isolation and identification of N-butyl-tetrahydro-5-oxofuran-2-carboxamide produced byBacillussp. L60 and its antifungal activity

2017 ◽  
Vol 57 (3) ◽  
pp. 283-288 ◽  
Author(s):  
Yong-Seong Lee ◽  
Jeong-Yong Cho ◽  
Jae-Hak Moon ◽  
Kil-Yong Kim
Keyword(s):  
Antifungal Activity ◽  
Isolation And Identification

scholarly journals Mycotoxins Content of Smoked Fishes Sampled on Market Stalls of Ngaoundere and Antifungal Activity of Essential Oils of Cymbopogon citratus and Ocimum basilicum against Mycotoxin Producing Strains

2020 ◽  
pp. 36-47
Author(s):  
Nadege D. Nganou ◽  
Eliane S. Tchinda ◽  
Alphonse T. Sokamte ◽  
Franklin K. Ngoualem ◽  
Steve F. Nodem ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Antifungal Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Ocimum Basilicum ◽  
Penicillium Citrinum ◽  
Cymbopogon Citratus ◽  
Isolation And Identification ◽  
Smoked Fish ◽  
Study Laboratory

Aim: The aim of this study was to evaluate the sanitary quality of smoked fish by determining their mycotoxin content, and also the chemical composition, antifungal activity of Cymbopogon citratus and Ocimum basilicum essential oils from Cameroon against some mycotoxigenic fungal strains responsible of the smoked fish biodegradation. Place and Duration of Study: Laboratory of Food Microbiology and Biotechnology, National School of Agro-Industrial Sciences, University of Ngaoundere, Cameroon, from August 2019 to April 2020. Methodology: Fifteen samples of smoked fishes have been collected in August 2019 from “petit marché” market (Ngaoundere, Cameroon). Physico-chemical parameters of smoked fishes were evaluated. Mycotoxin (AFB1, CIT, and OTA) contents have been determined with HPLC. Isolation and identification of molds were done using their macroscopic and microscopic characteristics, and the identity of the strains was done by PCR sequencing methods. For plants, 15 kg of Cymbopogon citratus leaves and 5 kg of Ocimum basilicum leaves have been harvested on 10 September 2019 in Ngaoundere and used for the essential oil’s extraction. Essential oil extraction has been done through hydrodistillation and the determination of its chemical composition done with GC/MS. Results: Major part of samples contains at least one mycotoxin, in quantities which are beyond the safe dose. Eleven species of molds have been identified: Aspergillus spp., Penicillium citrinum, and Mucor hiemalis. Amongst the isolated species, those which can produce mycotoxins are: Penicillium citrinum, Aspergillus versicolor, Aspergillus fumigatus, Aspergillus carbonarius, Aspergillus niger, and Fusarium moniliforme. Geranial (42.4%), Neral (33.5%) and Myrcene (10.8%) are major compounds found in the essential oil of C. citratus while monoterpens (60.8%), Eugenol (30.7%), Linalol (29.4%) and 1,8-Cineol (14.3%) are the major compounds found in the essential oil of O. basilicum.  Conclusion: Essential oils of C. citratus and O. basilicum are efficient against isolated toxigenic species. C. citratus being more efficient than O. basilicum.

Isolation and identification of a Bacillus megaterium strain with ochratoxin A removal ability and antifungal activity

Food Control ◽  
2019 ◽  
Vol 106 ◽  
pp. 106743 ◽  
Author(s):  
Linlin Shang ◽  
Xuexia Bai ◽  
Chi Chen ◽  
Lijun Liu ◽  
Mingyan Li ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Ochratoxin A ◽  
Bacillus Megaterium ◽  
Isolation And Identification

scholarly journals Effect of ozonized olive oil on oral levels of Candida spp. in patients with denture stomatitis

2018 ◽  
Vol 21 (1) ◽  
pp. 111 ◽  
Author(s):  
Erica Crastechini ◽  
Cristiane Yumi Koga-Ito ◽  
Suzan De Fátima Machado ◽  
Guilherme Rodrigues Teodoro ◽  
Graziella Nuernberg Back-Brito ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Olive Oil ◽  
In Vitro Tests ◽  
Candida Spp ◽  
Isolation And Identification ◽  
Denture Stomatitis ◽  
Viable Cells ◽  
In Vitro Antifungal Activity

<p><strong>Objective</strong>: The aim of this study was to evaluate the effect of ozonized oil (OZ) on the oral levels of <em>Candida </em>spp. in patients with denture stomatitis. <strong>Material and Methods</strong>: In vitro tests were performed to validate antifungal activity and to standardize OZ conditions. Antifungal activity was screened against <em>C. albicans </em>and five non-<em>albicans </em>species (<em>C. tropicalis, C. dubliniensis, C. krusei, C. guilliermondii,</em> and <em>C. parapsilosis</em>). Also, the effects on <em>C. albicans </em>planktonic and biofilm were evaluated. After validation, OZ was included in a therapeutic protocol of denture stomatitis in vivo. Thirty patients used OZ and 20 used sodium bicarbonate (SB) for 14 days. After 7 and 14 days, clinical evaluation, isolation and identification of yeasts were performed. Isolates were identified by phenotypic and genotypic tests. Ozonized oil showed in vitro antifungal activity against all species of <em>Candida</em>. Ozonized oil reduced the number of viable cells in <em>C. albicans </em>biofilms. Oral candidal levels were lower in relation to baseline both after after 14 days of treatment with SB and OZ. <strong>Results</strong>: A total of 493 <em>Candida </em>spp. isolates was obtained and 80% were identified as <em>C. albicans</em>. Remission of denture stomatitis was observed in all patients after 7 days of treatment in both groups. <strong>Conclusion</strong>: Within the limits of the study we can conclude that ozonized olive oil can be a new alternative for the control of biofilm in patients with denture stomatitis.</p><p align="left"> </p><p><strong>Keywords<em></em></strong></p><p>Ozone; <em>Candida</em>; Antifungal Agents; Stomatitis; Denture.</p><p> </p>

scholarly journals In vitro antifungal activity of Candida culture extracts against Trichophyton rubrum and Trichophyton mentagrophytes.

2021 ◽  
Vol 1 (4) ◽  
pp. 135-152
Author(s):  
Thiago Henrique Lemes ◽  
Guilherme Silva Torrezan ◽  
Carlos Roberto Polaquini ◽  
Luis Octavio Regasini ◽  
Bianca Gottardo de Almeida ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Galleria Mellonella ◽  
Trichophyton Rubrum ◽  
Fungal Infections ◽  
Trichophyton Mentagrophytes ◽  
Recurrence Rates ◽  
Isolation And Identification ◽  
Microdilution Method ◽  
Broth Microdilution Method

Onychomycoses are nail infections caused primarily by dermatophytes fungi, yeasts, and other filamentous fungi, characterized by persistent infections, prolonged therapy, and high recurrence rates. In clinical practice, some of these occurrences present two or more microorganisms, and the interactions among them can change the chemical environment mediated by small diffusible molecules, producing a competitive niche. The aim of this study was to evaluate the antifungal activity of individual extracts of pure cultures of Candida albicans and C. parapsilosis against dermatophytes. To obtain the fungal extracts, cultures were filtered through a 0.2 μm membrane and submitted to liquid-liquid extraction using ethyl acetate. The Minimal Inhibitory Concentration (MIC) of each extract was evaluated by broth microdilution method and checkerboard assay with fluconazole against clinical isolates of Trichophyton rubrum and T. mentagrophytes. The invertebrate model of Galleria mellonella was used to evaluate the toxicity of the extracts. As results, the extracts of C. albicans and C. parapsilosis showed antifungal activity with MICs between 31,2 – 2000 μg/mL. In association with fluconazole, synergistic effect was detected for all combinations. The extracts presented low toxicity in G. mellonella. In the future, isolation and identification of the extract compounds may allow new therapeutic approaches in the control of fungal infections.

scholarly journals In Vitro Antifungal Activity of Kampo Medicine Water Extracts against Trichophyton Rubrum

2016 ◽  
Vol 11 (6) ◽  
pp. 1934578X1601100 ◽  
Author(s):  
Xia Da ◽  
Hitoshi Takahashi ◽  
Kyaw Zaw Hein ◽  
Eishin Morita
Keyword(s):  
Antifungal Activity ◽  
Trichophyton Rubrum ◽  
Culture Media ◽  
Water Extract ◽  
Antifungal Drugs ◽  
Fungal Culture ◽  
Isolation And Identification ◽  
Antifungal Effect ◽  
Crude Extracts

Kampo medicines consist of a variety of crude animal, plant, and mineral extracts that have long been used to relieve different symptoms, and are relatively safe. However, their mechanisms of actions have not been well investigated. We screened 61 commercially available Kampo medicines to determine if they contain constituents with antifungal activity against Trichophyton rubrum. The antifungal effect of the Kampo medicines was determined by measuring the mean absorbance of treated fungal culture media. Lower absorbance values suggested a higher inhibition of the growth rate of T. rubrum by the Kampo medicines. We found that seven of the evaluated formulations exhibited a comparable antifungal activity to that of fluconazole at 14 mg/mL. The seven active Kampo medicines were Saiko-keishi-kankyou-to, Saiko-ka-ryukotsu-borei-to, Saiko-keishi-to, Keishi-ka-ryukotsu-borei-to, Dai-saiko-to, Bohu-tsu-sho-san, and Otsu-ji-to. The seven Kampo medicines with antifungal activity contain 30 different crude extracts, and Ou-gon (Scutellaria root) is a supplement contained in six of the seven formulations. Therefore, Ou-gon was considered to play a major role in their antifungal effect. The antifungal assay of the Ou-gon water extract showed that it significantly inhibited the growth of T. rubrum at a concentration of 20 mg/mL. Future studies will focus on the isolation and identification of the antifungal components of the crude extracts of Ou-gon, which may be potentially useful, new, and safe antifungal drugs.

Isolation and identification of 5-hydroxyl-5-methyl-2-hexenoic acid from Actinoplanes sp. HBDN08 with antifungal activity

2010 ◽  
Vol 101 (21) ◽  
pp. 8383-8388 ◽  
Author(s):  
Ji Zhang ◽  
Xiang-Jing Wang ◽  
Yi-Jun Yan ◽  
Ling Jiang ◽  
Ji-Dong Wang ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Isolation And Identification

scholarly journals Inhibitory activity of Rhamnolipid produced by Pseudomonas aeruginosa NH22 against Rhizoctonia solani

2012 ◽  
Vol 6 (1) ◽  
pp. 32-44
Author(s):  
Nadhem H. Hayder Gumaa
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antifungal Activity ◽  
Radial Growth ◽  
Laccase Activity ◽  
Dry Weight ◽  
Biosurfactant Production ◽  
Garden Soil ◽  
Isolation And Identification ◽  
Rf Values ◽  
Reduce Surface Tension

In the present study, isolation and identification of microorganisms from rhizospher of garden soil with biosurfactant producing ability and characterization of biosurfactants were studied. The results exhibited capability of four isolates for biosurfactant production among six isolates. The isolate Pseudomonas aeruginosa NH22 showed a better emulsifier producer, therefore it selected for biosurfactant production and antifungal activity in the present study. Biochemical analysis of partially purified bioemulsifier demonstrated that the biosurfactant contains mono, and di- rhamnolipid with Rf values of 0.82 and 0.32 respectively. Rhamnolipid efficiency tested for antifungal activity by determination percentage reduction of radial growth, fresh and dry weight and laccase activity of fungus. Maximum reduction in radial growth 76%, and reduction of biomass obtained at rhamnolipid concentration of 1000 µg/ ml. Maximum laccase activity obtained 0.921 U/ml also at rhamnolipid concentration of 1000 µg/ ml, while reduction about 21% of the enzyme activity recorded at higher rhamnolipih concentration of 1500 µg/ ml. The critical micelle concentration estimated to be 200 mg/l, which led to reduce surface tension value of the culture to34 mN/m.

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