Association of nerve growth factor mRNA levels with MK-801-induced explosive behaviors in mice

1995 ◽  
Vol 42 (1) ◽  
pp. 80-84
Author(s):  
L. A. Riley ◽  
A. Hitri ◽  
J. J. Bernstein
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Mrna Levels ◽  
Nerve Growth ◽  
Mk 801

Rescue of α-SNS Sodium Channel Expression in Small Dorsal Root Ganglion Neurons After Axotomy by Nerve Growth Factor In Vivo

1998 ◽  
Vol 79 (5) ◽  
pp. 2668-2676 ◽  
Author(s):  
S. D. Dib-Hajj ◽  
J. A. Black ◽  
T. R. Cummins ◽  
A. M. Kenney ◽  
J. D. Kocsis ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Dorsal Root Ganglion ◽  
Sodium Channel ◽  
Dorsal Root ◽  
Sodium Current ◽  
Mrna Levels ◽  
Drg Neurons ◽  
Nerve Growth

Dib-Hajj, S. D., J. A. Black, T. R. Cummins, A. M. Kenney, J. D. Kocsis, and S. G. Waxman. Rescue of α-SNS sodium channel expression in small dorsal root ganglion neurons after axotomy by nerve growth factor in vivo. J. Neurophysiol. 79: 2668–2676, 1998. Small (18–25 μm diam) dorsal root ganglion (DRG) neurons are known to express high levels of tetrodotoxin-resistant (TTX-R) sodium current and the mRNA for the α-SNS sodium channel, which encodes a TTX-R channel when expressed in oocytes. These neurons also preferentially express the high affinity receptor for nerve growth factor (NGF), TrkA. Levels of TTX-R sodium current and of α-SNS mRNA are reduced in these cells after axotomy. To determine whether NGF participates in the regulation of TTX-R current and α-SNS mRNA in small DRG neurons in vivo, we axotomized small lumbar DRG neurons by sciatic nerve transection and administered NGF or Ringer solution to the proximal nerve stump using osmotic pumps. Ten to 12 days after pump implant, whole cell patch-clamp recording demonstrated that TTX-R current density was decreased in Ringer-treated axotomized neurons (154 ± 45 pA/pF; mean ± SE) compared with nonaxotomized control neurons (865 ± 123 pA/pF) and was restored partially toward control levels in NGF-treated axotomized neurons (465 ± 78 pA/pF). The V 1/2 for steady-state activation and inactivation of TTX-R currents were similar in control, Ringer- and NGF-treated axotomized neurons. Reverse transcription polymerase chain reaction revealed an upregulation of α-SNS mRNA levels in NGF-treated compared with Ringer-treated axotomized DRG. In situ hybridization showed that α-SNS mRNA levels were decreased significantly in small Ringer-treated axotomized DRG neurons in vivo and also in small DRG neurons that were dissociated and maintained in vitro, so as to correspond to the patch-clamp conditions. NGF-treated axotomized neurons had a significant increase in α-SNS mRNA expression, compared with Ringer-treated axotomized cells. These results show that the administration of exogenous NGF in vivo, to the proximal nerve stump of the transected sciatic nerve, results in an upregulation of TTX-R sodium current and of α-SNS mRNA levels in small DRG neurons. Retrogradely transported NGF thus appears to participate in the control of excitability in these cells via actions that include the regulation of sodium channel gene expression in vivo.

Short- and long-term mechanisms of tau regulation in PC12 cells

1995 ◽  
Vol 108 (8) ◽  
pp. 2857-2864 ◽  
Author(s):  
E. Sadot ◽  
J. Barg ◽  
D. Rasouly ◽  
P. Lazarovici ◽  
I. Ginzburg
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Pc12 Cells ◽  
Tau Protein ◽  
Immunoblot Analysis ◽  
Term Treatment ◽  
Mrna Levels ◽  
Short Term Treatment ◽  
Nerve Growth ◽  
Protein Levels

Induction by nerve growth factor of neurite outgrowth in PC12 cells is transcription-dependent and is associated with the accumulation of tau protein. It was recently shown that short-term treatment with staurosporine, a protein kinase alkaloid inhibitor, induced an elevation of tau protein levels and outgrowth of stable neurites. In this study, we analyzed the mechanism(s) by which nerve growth factor and staurosporine exert their effects on tau levels. We demonstrate that nerve growth factor affects tau mRNA stability, thus contributing to the observed increase in tau mRNA levels. On the other hand, tau mRNA levels were not affected by the treatment with staurosporine. We also demonstrate that the phosphorylation of tau protein was reduced after treatment of PC12 cells with nerve growth factor or staurosporine, as shown by immunoblot analysis using specific antibodies and alkaline phosphatase treatment. Thus, regulation of tau levels by nerve growth factor appears to be mediated by transcriptional, post-transcriptional and posttranslational steps, whereas the effect of staurosporine on tau levels may be attributed to its effect on the state of phosphorylation of the protein.

Effect of protein synthesis inhibitors on growth factor activation of c-fos, c-myc, and actin gene transcription

1986 ◽  
Vol 6 (4) ◽  
pp. 1050-1057
Author(s):  
M E Greenberg ◽  
A L Hermanowski ◽  
E B Ziff
Keyword(s):  
Protein Synthesis ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Pc12 Cells ◽  
Gene Transcription ◽  
Actin Gene ◽  
Mrna Levels ◽  
Protein Synthesis Inhibitors ◽  
3T3 Cells ◽  
Nerve Growth

Stimulation of quiescent 3T3 cells with purified growth factors or of the pheochromocytoma cell line PC12 with nerve growth factor results in the rapid transient induction of c-fos, c-myc, and actin gene transcription (M.E. Greenberg and E.B. Ziff, Nature [London] 312:711-716; M.E. Greenberg, L.A. Greene, and E.B. Ziff, J. Biol. Chem. 26:14101-14110). We used protein synthesis inhibitors to investigate whether synthesis of new proteins plays a role in the rapid induction and subsequent repression of the transcription of these genes. Pretreatment of quiescent 3T3 cells with the inhibitor anisomycin before growth factor stimulation caused a superinduction of c-fos and c-myc mRNA levels upon growth factor addition. Nuclear runoff transcription analyses of 3T3 cells indicated that anisomycin potentiated c-fos, c-myc, and also actin expression at the transcriptional level, possibly by inhibiting transcriptional repression. Somewhat different results were obtained when PC12 cells were incubated with either anisomycin or cycloheximide. In PC12 cells protein synthesis inhibitors superinduced nerve growth factor activation of c-fos mRNA production but completely abolished the activation of c-myc. The results suggest that in PC12 cells c-fos transcription is activated by a protein-synthesis-independent mechanism, whereas c-myc stimulation requires new protein synthesis. The difference in the effect of anisomycin on growth factor activation of c-myc expression in 3T3 versus PC12 cells may be due to differential stringency of protein synthesis inhibition in the two cells or could reflect cell type differences in c-myc regulation.

Effects of nerve crush and transection on mRNA levels for nerve growth factor receptor in the rat facial motoneurons

1991 ◽  
Vol 9 (1-2) ◽  
pp. 157-160 ◽  
Author(s):  
Takanori Saika ◽  
Emiko Senba ◽  
Koichi Noguchi ◽  
Makoto Sato ◽  
Shigetaka Yoshida ◽  
...  
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Growth Factor Receptor ◽  
Nerve Growth Factor Receptor ◽  
Mrna Levels ◽  
Nerve Crush ◽  
Nerve Growth ◽  
Facial Motoneurons

Nerve growth factor promotes neurite outgrowth in guinea pig myenteric plexus ganglia

1994 ◽  
Vol 267 (4) ◽  
pp. G716-G722
Author(s):  
M. W. Mulholland ◽  
G. Romanchuk ◽  
K. Lally ◽  
D. M. Simeone
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Guinea Pig ◽  
Myenteric Plexus ◽  
Enteric Neurons ◽  
Morphological Development ◽  
Mrna Levels ◽  
Neurite Length ◽  
Nerve Growth ◽  
Neurite Density

Nerve growth factor (NGF) has important developmental actions in both central and peripheral nervous systems. Primary cultures of neonatal guinea pig myenteric plexus ganglia were used to examine the ability of NGF to stimulate morphological development in enteric neurons. NGF, in the presence of a serum-free medium, produced dose-dependent increases in neurite density, significant at 1 ng/ml and maximal at 100 ng/ml (4.5-fold increase vs. control). Maximum neurite length was also significantly increased at 1 ng/ml, with maximal effects at 100 ng/ml. Coincubation of NGF (50 ng/ml) with monoclonal NGF antibodies abolished increases in both neurite density (128 +/- 19 processes/mm for control, 369 +/- 19 for NGF, 183 +/- 28 for NGF+monoclonal antibodies) and neurite length. Exposure of enteric neurons to low concentrations of NGF (1 ng/ml) was also associated with increased mRNA levels for cytoskeletal genes. alpha-Tubulin mRNA levels were increased 3.9 +/- 0.7 times basal at 48 h. mRNA levels for microtubule-associated protein 2 were increased threefold at 48 h of NGF incubation. NGF demonstrates activities in cultured enteric ganglia that stimulate morphological development.

scholarly journals A Unique Pattern of Bisphenol A Effects on Nerve Growth Factor Gene Expression in Embryonic Mouse Hypothalamic Cell Line N-44

2014 ◽  
Vol 65 (3) ◽  
pp. 293-299 ◽  
Author(s):  
Katsuhiko Warita ◽  
Tomoko Mitsuhashi ◽  
Nobuhiko Hoshi ◽  
Ken-ichi Ohta ◽  
Shingo Suzuki ◽  
...  
Keyword(s):  
Gene Expression ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Bisphenol A ◽  
Cell Line ◽  
Mrna Levels ◽  
Dependent Manner ◽  
Embryonic Mouse ◽  
Nerve Growth ◽  
Unique Pattern

AbstractWe investigated the toxicity of bisphenol A (BPA) by determining the gene expression of nerve growth factor (Ngf in the embryonic mouse cell line mHypoE-N44 derived from the hypothalamus exposed to BPA dose range between 0.02 and 200 μmol L-1 for 3 h. Ngf mRNA levels decreased in a dose-dependent manner, with significant reductions observed in the 2 to 50 μmol L-1 BPA treatment groups compared to controls. However, at 100 to 200 μmol L-1 the NgfmRNA gradually increased and was significantly higher than control, while the expression of the apoptosis-related genes Caspase 3 and transformation-related protein 73 decreased significantly. These results suggest that in an embryonic hypothalamic cell line the higher doses of BPA induce a unique pattern of Ngf gene expression and that BPA has the potential to suppress apoptosis essential for early-stage brain development.

scholarly journals Prohormone Convertases in Mouse Submandibular Gland: Co-localization of Furin and Nerve Growth Factor

1997 ◽  
Vol 45 (6) ◽  
pp. 795-804 ◽  
Author(s):  
Hooman Farhadi ◽  
Sangeeta Pareek ◽  
Robert Day ◽  
Weijia Dong ◽  
Michel Chrétien ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Proteolytic Enzymes ◽  
Mrna Levels ◽  
Prohormone Convertases ◽  
Nerve Growth ◽  
Submandibular Glands ◽  
Ductal Cells

Nerve growth factor (NGF) in mouse submandibular glands (SGs) is generated from a 35-kD precursor by proteolytic enzymes that have yet to be identified. Prohormone convertases (PCs) cleave the NGF precursor in vitro, and in this study we questioned whether PCs could process salivary NGF in vivo. mRNA coding for PC2 (but not PC1) was detected on Northern blots of SG mRNA and also by in situ hybridization within parasympathetic neurons of intralobular ganglia. Northern blot and in situ hybridization analyses also detect mRNA coding for furin. In SGs of male mice, furin mRNA levels are high at birth and remain high throughout development. In glands from female mice, levels decline during postnatal development and are lower in adults than in newborns. Immunocytochemistry detects furin immunoreactivity in pro-acinar and ductal cells of glands from newborn and pubescent mice. In glands of adults, furin immunoreactivity is detectable in acinar cells but highest levels are present in NGF-containing granular convoluted tubule cells. These data, taken together with those from previous studies, suggest that furin is a candidate processing enzyme for NGF in mouse submandibular glands.

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