Quantitative brain autoradiography of [9, 10-3H]palmitic acid incorporation into brain lipids

1990 ◽  
Vol 26 (2) ◽  
pp. 196-208 ◽  
Author(s):  
J. G. Noronha ◽  
J. M. Bell ◽  
S. I. Rapoport
1962 ◽  
Vol 40 (9) ◽  
pp. 1229-1238 ◽  
Author(s):  
K. K. Carroll

Palmitic acid -1-C14, erucic acid -2-C14, and nervonic acid -2-C14 were administered to young adult rats by mouth, and levels of radioactivity in various body tissues and in respiratory CO2 were determined. After [Formula: see text] hours about 20% of the label from palmitic acid and erucic acid and 30% from nervonic acid was still in the intestinal tract but after 24 hours less than 5% remained. At [Formula: see text] or 24 hours, tissue lipids of rats given palmitic acid generally contained a higher percentage of radioactivity administered than lipids from corresponding tissues of rats given erucic acid or nervonic acid. Very little radioactivity was found in brain lipids of either adult or 12-day-old rats given the labelled acids. The fatty acids were also given to adult rats by tail-vein injection and highest activities were again observed in tissue lipids of rats given palmitic acid.When given by mouth, palmitic acid contributed more radioactivity to respiratory CO2 during the first 6 hours than erucic acid or nervonic acid, and peak activities did not occur until 2 to 3 hours after administration. When given by tail vein, erucic acid and nervonic acid contributed more activity to respiratory CO2 than palmitic acid. Highest activities were observed during the first hour but the peak was reached somewhat later in rats given erucic acid or nervonic acid.


1962 ◽  
Vol 40 (1) ◽  
pp. 1229-1238 ◽  
Author(s):  
K. K. Carroll

Palmitic acid -1-C14, erucic acid -2-C14, and nervonic acid -2-C14 were administered to young adult rats by mouth, and levels of radioactivity in various body tissues and in respiratory CO2 were determined. After [Formula: see text] hours about 20% of the label from palmitic acid and erucic acid and 30% from nervonic acid was still in the intestinal tract but after 24 hours less than 5% remained. At [Formula: see text] or 24 hours, tissue lipids of rats given palmitic acid generally contained a higher percentage of radioactivity administered than lipids from corresponding tissues of rats given erucic acid or nervonic acid. Very little radioactivity was found in brain lipids of either adult or 12-day-old rats given the labelled acids. The fatty acids were also given to adult rats by tail-vein injection and highest activities were again observed in tissue lipids of rats given palmitic acid.When given by mouth, palmitic acid contributed more radioactivity to respiratory CO2 during the first 6 hours than erucic acid or nervonic acid, and peak activities did not occur until 2 to 3 hours after administration. When given by tail vein, erucic acid and nervonic acid contributed more activity to respiratory CO2 than palmitic acid. Highest activities were observed during the first hour but the peak was reached somewhat later in rats given erucic acid or nervonic acid.


1990 ◽  
Vol 29 (2) ◽  
pp. 409-415
Author(s):  
M. Gautier ◽  
P. Moreau ◽  
J. Bessoule ◽  
R. Lessire ◽  
C. Anding ◽  
...  

2011 ◽  
Vol 98 (3) ◽  
pp. 359-366 ◽  
Author(s):  
M. Synak ◽  
R. Zarzeczny ◽  
M. Górecka ◽  
J. Langfort ◽  
H. Kaciuba-Uściłko ◽  
...  

2003 ◽  
Vol 81 (1) ◽  
pp. 35-41 ◽  
Author(s):  
M Synak ◽  
M Górecka ◽  
J Langfort ◽  
E Smol ◽  
E Zernicka

Palmitic acid incorporation into the intramuscular acylglycerols in rat skeletal muscles of different fiber types was investigated at various total and unbound to albumin concentrations by means of the hind-limb perfusion technique. It was found that at simultaneously increasing total and unbound to albumin palmitic acid concentrations in the perfusion medium the incorporation of palmitic acid into acylglycerols increased. However, when the concentration of palmitic acid not bound to albumin was kept constant and the total palmitic acid concentration was increased, the incorporation also increased although markedly less than under former conditions. The increase was most apparent in the muscles composed of slow-twitch oxidative and fast-twitch oxidative-glycolytic fibers where fatty acid uptake is the greatest. These findings suggest that fatty acid incorporation into intramuscular acylglycerols depends not only on the unbound to albumin fatty acid concentration but also, to some extent, on the total fatty acid concentration.Key words: skeletal muscle, perfusion, fatty acid uptake.


2011 ◽  
Author(s):  
Katrin Legg
Keyword(s):  

Diabetes ◽  
2020 ◽  
Vol 69 (Supplement 1) ◽  
pp. 1832-P
Author(s):  
ANNA SANTORO ◽  
PENG ZHOU ◽  
YAN ZHU ◽  
ODILE D. PERONI ◽  
ANDREW T. NELSON ◽  
...  

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