Early and late appearance of neurofilament phosphorylated epitopes in rat nervous system development: In vivo and in vitro study with monoclonal antibodies

1988 ◽  
Vol 20 (4) ◽  
pp. 431-441 ◽  
Author(s):  
D. Dahl
Keyword(s):  
Nervous System ◽  
Monoclonal Antibodies ◽  
System Development ◽  
In Vitro Study ◽  
Nervous System Development ◽  
Vitro Study

scholarly journals Drosophila Stathmin: A Microtubule-destabilizing Factor Involved in Nervous System Formation

2002 ◽  
Vol 13 (2) ◽  
pp. 698-710 ◽  
Author(s):  
Sylvie Ozon ◽  
Antoine Guichet ◽  
Olivier Gavet ◽  
Siegfried Roth ◽  
André Sobel
Keyword(s):  
Nervous System ◽  
System Development ◽  
Nervous System Development ◽  
Microtubule Assembly ◽  
Nervous Systems ◽  
Germ Cell Migration ◽  
Numerous Cell ◽  
First Time

Stathmin is a ubiquitous regulatory phosphoprotein, the generic element of a family of neural phosphoproteins in vertebrates that possess the capacity to bind tubulin and interfere with microtubule dynamics. Although stathmin and the other proteins of the family have been associated with numerous cell regulations, their biological roles remain elusive, as in particular inactivation of the stathmin gene in the mouse resulted in no clear deleterious phenotype. We identified stathmin phosphoproteins inDrosophila, encoded by a unique gene sharing the intron/exon structure of the vertebrate stathmin andstathmin family genes. They interfere with microtubule assembly in vitro, and in vivo when expressed in HeLa cells. Drosophila stathmin expression is regulated during embryogenesis: it is high in the migrating germ cells and in the central and peripheral nervous systems, a pattern resembling that of mammalian stathmin. Furthermore, RNA interference inactivation ofDrosophila stathmin expression resulted in germ cell migration arrest at stage 14. It also induced important anomalies in nervous system development, such as loss of commissures and longitudinal connectives in the ventral cord, or abnormal chordotonal neuron organization. In conclusion, a single Drosophilagene encodes phosphoproteins homologous to the entire vertebrate stathmin family. We demonstrate for the first time their direct involvement in major biological processes such as development of the reproductive and nervous systems.

scholarly journals CD60b: Enriching Neural Stem/Progenitor Cells from Rat Development into Adulthood

2017 ◽  
Vol 2017 ◽  
pp. 1-16
Author(s):  
Fernanda Gubert ◽  
Camila Zaverucha-do-Valle ◽  
Michelle Furtado ◽  
Pedro M. Pimentel-Coelho ◽  
Nicoli Mortari ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Progenitor Cells ◽  
System Development ◽  
Nervous System Development ◽  
Adult Brain ◽  
Neurogenic Niche ◽  
Neural Stem Progenitor Cells

CD60b antigens are highly expressed during development in the rat nervous system, while in the adult their expression is restricted to a few regions, including the subventricular zone (SVZ) around the lateral ventricles—a neurogenic niche in the adult brain. For this reason, we investigated whether the expression of C60b is associated with neural stem/progenitor cells in the SVZ, from development into adulthood. We performedin vitroandin vivoanalyses of CD60b expression at different stages and identified the presence of these antigens in neural stem/progenitor cells. We also observed that CD60b could be used to purify and enrich a population of neurosphere-forming cells from the developing and adult brain. We showed that CD60b antigens (mainly corresponding to ganglioside 9-O-acetyl GD3, a well-known molecule expressed during central nervous system development and mainly associated with neuronal migration) are also present in less mature cells and could be used to identify and isolate neural stem/progenitor cells during development and in the adult brain. A better understanding of molecules associated with neurogenesis may contribute not only to improve the knowledge about the physiology of the mammalian central nervous system, but also to find new treatments for regenerating tissue after disease or brain injury.

Effects of TSH and calcitriol on bone metabolism: in vivo and in vitro study

2014 ◽  
Author(s):  
Ivo Dumic-Cule ◽  
Dunja Rogic ◽  
Damir Jezek ◽  
Lovorka Grgurevic ◽  
Slobodan Vukicevic
Keyword(s):  
Bone Metabolism ◽  
In Vitro Study ◽  
Vitro Study

Inhibition of Cyclosporin A-Induced Gingival Overgrowth by Azithromycin Through Phagocytosis: An In Vivo and In Vitro Study

2004 ◽  
Vol 75 (3) ◽  
pp. 380-387 ◽  
Author(s):  
Jeong-Won Paik ◽  
Chang-Sung Kim ◽  
Kyoo-Sung Cho ◽  
Jung-Kiu Chai ◽  
Chong-Kwan Kim ◽  
...  
Keyword(s):  
Cyclosporin A ◽  
In Vitro Study ◽  
Vitro Study ◽  
Gingival Overgrowth

In-vivo and in-vitro study of control of rat skin optical properties by action of 40%-glucose solution

2001 ◽  
Author(s):  
Alexey N. Bashkatov ◽  
Elina A. Genina ◽  
Irina V. Korovina ◽  
Yurii P. Sinichkin ◽  
Olga V. Novikova ◽  
...  
Keyword(s):  
Optical Properties ◽  
Glucose Solution ◽  
In Vitro Study ◽  
Vitro Study ◽  
Rat Skin

In vivo and in vitro Study of V Uptake in Developing Rat Molar Enamel

1980 ◽  
Vol 59 (10) ◽  
pp. 1643-1648 ◽  
Author(s):  
J.W. Bawden ◽  
T.G. Deaton ◽  
M. Chavis
Keyword(s):  
In Vitro Study ◽  
Vitro Study ◽  
Developing Rat ◽  
Rat Molar
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