FRET‐based imaging of intracellular ATP in organotypic brain slices

2018 ◽  
Vol 97 (8) ◽  
pp. 933-945 ◽  
Author(s):  
Rodrigo Lerchundi ◽  
Karl W. Kafitz ◽  
Ulrike Winkler ◽  
Marcel Färfers ◽  
Johannes Hirrlinger ◽  
...  
Keyword(s):  
Brain Slices ◽  
Intracellular Atp ◽  
Organotypic Brain Slices

NGF but not GDNF or neurturin enhance acetylcholine tissue levels in striatal organotypic brain slices

1998 ◽  
Vol 16 (5) ◽  
pp. 391-401 ◽  
Author(s):  
H.P. Fischer ◽  
J. Marksteiner ◽  
G. Ransmayr ◽  
A. Saria ◽  
C. Humpel
Keyword(s):  
Brain Slices ◽  
Tissue Levels ◽  
Organotypic Brain Slices

scholarly journals Real‐time monitoring of oxygenation in cultured organotypic brain slices (1180.20)

2014 ◽  
Vol 28 (S1) ◽  
Author(s):  
Ruslan Dmitriev ◽  
Sergey Borisov ◽  
Alina Kondrashina ◽  
Janelle Pakan ◽  
Dmitri Papkovsky
Keyword(s):  
Real Time ◽  
Brain Slices ◽  
Real Time Monitoring ◽  
Organotypic Brain Slices

scholarly journals Bioorthogonal labeling of transmembrane proteins with non-canonical amino acids allows access to masked epitopes in live neurons

2021 ◽  
Author(s):  
Diogo Bessa-Neto ◽  
Alexander Kuhlemann ◽  
Gerti Beliu ◽  
Valeria Pecoraro ◽  
Sören Doose ◽  
...  
Keyword(s):  
Amino Acids ◽  
Brain Slices ◽  
Transmembrane Proteins ◽  
Super Resolution ◽  
Biological Imaging ◽  
Bioorthogonal Labeling ◽  
Resolution Imaging ◽  
Genetic Code Expansion ◽  
Super Resolution Microscopy ◽  
Organotypic Brain Slices

ABSTRACTProgress in biological imaging is intrinsically linked to advances in labeling methods. The explosion in the development of high-resolution and super-resolution imaging calls for new approaches to label targets with small probes. These should allow to faithfully report the localization of the target within the imaging resolution – typically nowadays a few nanometers - and allow access to any epitope of the target, in the native cellular and tissue environment. We report here the development of a complete labeling and imaging pipeline using genetic code expansion and non-canonical amino acids in primary neurons that allows to fluorescently label masked epitopes in target transmembrane proteins in live neurons, both in dissociated culture and organotypic brain slices. This allowed us to image the differential localization of two glutamate receptor auxiliary proteins in complex with their partner with a variety of methods including widefield, confocal, and dSTORM super-resolution microscopy.

Construction and application of a microprojectile system for the transfection of organotypic brain slices

2000 ◽  
Vol 101 (2) ◽  
pp. 171-179 ◽  
Author(s):  
Merdol Ibrahim ◽  
Azeddine Si-Ammour ◽  
Marco R Celio ◽  
Felix Mauch ◽  
Pierre-Alain Menoud
Keyword(s):  
Brain Slices ◽  
Organotypic Brain Slices
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