scholarly journals Energy metabolism in the rat cortex under thiopental anaesthesia measuredIn Vivoby13C MRS

2017 ◽  
Vol 95 (11) ◽  
pp. 2297-2306 ◽  
Author(s):  
Sarah Sonnay ◽  
João M. N. Duarte ◽  
Nathalie Just ◽  
Rolf Gruetter
Keyword(s):  
Energy Metabolism ◽  
Rat Cortex

scholarly journals Compartmentalised energy metabolism supporting glutamatergic neurotransmission in response to increased activity in the rat cerebral cortex: A 13C MRS study in vivo at 14.1 T

2016 ◽  
Vol 36 (5) ◽  
pp. 928-940 ◽  
Author(s):  
Sarah Sonnay ◽  
João MN Duarte ◽  
Nathalie Just ◽  
Rolf Gruetter
Keyword(s):  
Energy Metabolism ◽  
Tca Cycle ◽  
Resonance Spectroscopy ◽  
Activity Levels ◽  
Rat Cortex ◽  
Metabolic Compartmentation ◽  
Specific Regulation ◽  
A Minor ◽  
Stimulation Of

Many tissues exhibit metabolic compartmentation. In the brain, while there is no doubt on the importance of functional compartmentation between neurons and glial cells, there is still debate on the specific regulation of pathways of energy metabolism at different activity levels. Using 13C magnetic resonance spectroscopy (MRS) in vivo, we determined fluxes of energy metabolism in the rat cortex under α-chloralose anaesthesia at rest and during electrical stimulation of the paws. Compared to resting metabolism, the stimulated rat cortex exhibited increased glutamate–glutamine cycle (+67 nmol/g/min, +95%, P < 0.001) and tricarboxylic (TCA) cycle rate in both neurons (+62 nmol/g/min, +12%, P < 0.001) and astrocytes (+68 nmol/g/min, +22%, P = 0.072). A minor, non-significant modification of the flux through pyruvate carboxylase was observed during stimulation (+5 nmol/g/min, +8%). Altogether, this increase in metabolism amounted to a 15% (67 nmol/g/min, P < 0.001) increase in CMRglc(ox), i.e. the oxidative fraction of the cerebral metabolic rate of glucose. In conclusion, stimulation of the glutamate–glutamine cycle under α-chloralose anaesthesia is associated to similar enhancement of neuronal and glial oxidative metabolism.

scholarly journals The contribution of GABA to glutamate/glutamine cycling and energy metabolism in the rat cortex in vivo

2005 ◽  
Vol 102 (15) ◽  
pp. 5588-5593 ◽  
Author(s):  
A. B. Patel ◽  
R. A. de Graaf ◽  
G. F. Mason ◽  
D. L. Rothman ◽  
R. G. Shulman ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Rat Cortex

Mitochondrial matrix calcium ions regulate energy production in myocardium: A cytochemical study

1990 ◽  
Vol 48 (2) ◽  
pp. 166-167
Author(s):  
W.A. Jacob ◽  
R. Hertsens ◽  
A. Van Bogaert ◽  
M. De Smet
Keyword(s):  
Energy Metabolism ◽  
Calcium Ions ◽  
Energy Production ◽  
Regulation Of Respiration ◽  
Free Calcium ◽  
Mitochondrial Matrix ◽  
Cytochemical Study ◽  
Nmr Techniques

In the past most studies of the control of energy metabolism focus on the role of the phosphorylation potential ATP/ADP.Pi on the regulation of respiration. Studies using NMR techniques have demonstrated that the concentrations of these compounds for oxidation phosphorylation do not change appreciably throughout the cardiac cycle and during increases in cardiac work. Hence regulation of energy production by calcium ions, present in the mitochondrial matrix, has been the object of a number of recent studies.Three exclusively intramitochondnal dehydrogenases are key enzymes for the regulation of oxidative metabolism. They are activated by calcium ions in the low micromolar range. Since, however, earlier estimates of the intramitochondnal calcium, based on equilibrium thermodynamic considerations, were in the millimolar range, a physiological correlation was not evident. The introduction of calcium-sensitive probes fura-2 and indo-1 made monitoring of free calcium during changing energy metabolism possible. These studies were performed on isolated mitochondria and extrapolation to the in vivo situation is more or less speculative.

Soy isoflavone Genistein affects energy metabolism in a steatotic liver model

2012 ◽  
Vol 50 (01) ◽  
Author(s):  
S Lünse ◽  
A Krüger ◽  
M Glanemann ◽  
G Damm
Keyword(s):  
Energy Metabolism ◽  
Soy Isoflavone ◽  
Steatotic Liver ◽  
Liver Model

Regulation of energy metabolism in hepatocytes with induced steatosis

2015 ◽  
Vol 53 (01) ◽  
Author(s):  
K Schönefeld ◽  
M Matz-Soja ◽  
J Böttger ◽  
P Seibel ◽  
R Gebhardt
Keyword(s):  
Energy Metabolism
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