Adipocytes derived from PA6 cells reliably promote the differentiation of dopaminergic neurons from human embryonic stem cells

2014 ◽  
Vol 92 (5) ◽  
pp. 564-573 ◽  
Author(s):  
M.O. Guloglu ◽  
A. Larsen ◽  
P. Brundin
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Dopaminergic Neurons ◽  
Embryonic Stem

Efficient Differentiation of Human Embryonic Stem Cells Toward Dopaminergic Neurons Using Recombinant LMX1A Factor

2014 ◽  
Vol 57 (2) ◽  
pp. 184-194 ◽  
Author(s):  
Ali Fathi ◽  
Hassan Rasouli ◽  
Meghdad Yeganeh ◽  
Ghassem Hosseini Salekdeh ◽  
Hossein Baharvand
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Dopaminergic Neurons ◽  
Embryonic Stem

scholarly journals Enhanced Yield of Neuroepithelial Precursors and Midbrain-Like Dopaminergic Neurons from Human Embryonic Stem Cells Using the Bone Morphogenic Protein Antagonist Noggin

Stem Cells ◽  
2007 ◽  
Vol 25 (2) ◽  
pp. 411-418 ◽  
Author(s):  
Kai-Christian Sonntag ◽  
Jan Pruszak ◽  
Takahito Yoshizaki ◽  
Joris van Arensbergen ◽  
Rosario Sanchez-Pernaute ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Dopaminergic Neurons ◽  
Embryonic Stem ◽  
Bone Morphogenic Protein ◽  
Enhanced Yield

scholarly journals Differentiation of Human Embryonic Stem Cells to Dopaminergic Neurons in Serum-Free Suspension Culture

Stem Cells ◽  
2004 ◽  
Vol 22 (7) ◽  
pp. 1218-1238 ◽  
Author(s):  
Thomas C. Schulz ◽  
Scott A. Noggle ◽  
Gail M. Palmarini ◽  
Deb A. Weiler ◽  
Ian G. Lyons ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Suspension Culture ◽  
Human Embryonic Stem Cells ◽  
Dopaminergic Neurons ◽  
Embryonic Stem ◽  
Serum Free ◽  
Serum Free Suspension

scholarly journals Neural Progenitor Cells Derived from Human Embryonic Stem Cells as an Origin of Dopaminergic Neurons

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Parinya Noisa ◽  
Taneli Raivio ◽  
Wei Cui
Keyword(s):  
Parkinson’S Disease ◽  
Stem Cells ◽  
Parkinson's Disease ◽  
Embryonic Stem Cells ◽  
Progenitor Cells ◽  
Human Embryonic Stem Cells ◽  
Dopaminergic Neurons ◽  
Neural Progenitor Cells ◽  
Embryonic Stem ◽  
Neural Progenitor

Human embryonic stem cells (hESCs) are able to proliferatein vitroindefinitely without losing their ability to differentiate into multiple cell types upon exposure to appropriate signals. Particularly, the ability of hESCs to differentiate into neuronal subtypes is fundamental to develop cell-based therapies for several neurodegenerative disorders, such as Alzheimer’s disease, Huntington’s disease, and Parkinson’s disease. In this study, we differentiated hESCs to dopaminergic neurons via an intermediate stage, neural progenitor cells (NPCs). hESCs were induced to neural progenitor cells by Dorsomorphin, a small molecule that inhibits BMP signalling. The resulting neural progenitor cells exhibited neural bipolarity with high expression of neural progenitor genes and possessed multipotential differentiation ability. CBF1 and bFGF responsiveness of these hES-NP cells suggested their similarity to embryonic neural progenitor cells. A substantial number of dopaminergic neurons were derived from hES-NP cells upon supplementation of FGF8 and SHH, key dopaminergic neuron inducers. Importantly, multiple markers of midbrain neurons were detected, includingNURR1, PITX3, andEN1, suggesting that hESC-derived dopaminergic neurons attained the midbrain identity. Altogether, this work underscored the generation of neural progenitor cells that retain the properties of embryonic neural progenitor cells. These cells will serve as an unlimited source for the derivation of dopaminergic neurons, which might be applicable for treating patients with Parkinson’s disease.

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