Therapeutic effects of human adipose stem cell-conditioned medium on stroke

2012 ◽  
Vol 90 (9) ◽  
pp. 1794-1802 ◽  
Author(s):  
Yu Jin Cho ◽  
Hyeon Suk Song ◽  
SukHo Bhang ◽  
Saehyoung Lee ◽  
Bong Gu Kang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Conditioned Medium ◽  
Therapeutic Effects ◽  
Adipose Stem Cell ◽  
Human Adipose Stem Cell ◽  
Cell Conditioned Medium

scholarly journals High Frequency of Intravenous Injection of Human Adipose Stem Cell Conditioned Medium Improved Embryo Development of Mice in Advanced Maternal Age through Antioxidant Effects

Animals ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 978 ◽  
Author(s):  
Kihae Ra ◽  
Hyun Ju Oh ◽  
Geon A Kim ◽  
Sung Keun Kang ◽  
Jeong Chan Ra ◽  
...  
Keyword(s):  
Stem Cell ◽  
Conditioned Medium ◽  
Embryo Development ◽  
High Frequency ◽  
Maternal Age ◽  
Advanced Maternal Age ◽  
Adipose Stem Cell ◽  
Old Mice ◽  
Human Adipose Stem Cell ◽  
Cell Conditioned Medium

Advanced maternal age (AMA) has become prevalent globally. With aging, weakened antioxidant defense causes loss of normal function in the ovary and uterus due to oxidative stress. Here, we aimed to improve embryo development in AMA mice by intravenous injection (IV) of human adipose stem cell conditioned medium (ASC-CM) at various frequencies and intervals as an antioxidant intervention. Four- and six-month-old female ICR (Institute of Cancer Research) mice were randomly divided into groups IV treated with human ASC-CM under different conditions, and in vitro and in vivo embryo development were evaluated. Consequently, compared to the control group, blastocyst formation rate of parthenotes was significantly promoted in 4-month-old mice and the mean number of implanted fetuses after natural mating was significantly increased by approximately two-fold in 6-month-old mice. Through gene analysis, the anti-apoptotic and anti-oxidative effects of human ASC-CMs were confirmed in the ovaries and uterus of pregnant mice at both ages. In particular, ovarian expression of gpx1 and catalase drastically increased in 6-month-old mice. Furthermore, the levels of gpx1 and catalase were further increased, with a high frequency of injection regardless of age. Thus, we demonstrated for the first time the anti-oxidative effect of human ASC-CM administration against ovarian aging and the optimal injection condition.

scholarly journals The Effects of Cytokines in Adipose Stem Cell-Conditioned Medium on the Migration and Proliferation of Skin FibroblastsIn Vitro

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Jiajia Zhao ◽  
Li Hu ◽  
Jiarong Liu ◽  
Niya Gong ◽  
Lili Chen
Keyword(s):  
Wound Healing ◽  
Stem Cell ◽  
Conditioned Medium ◽  
Healing Process ◽  
Dermal Fibroblasts ◽  
Wound Healing Process ◽  
Adipose Stem Cell ◽  
And Migration ◽  
Cell Conditioned Medium ◽  
Proliferation And Migration

Although adipose stem cell-conditioned medium (ASC-CM) has demonstrated the effect of promoting the cutaneous wound healing, the mechanism for this response on the effector cells (e.g., dermal fibroblasts) during the process remains to be determined. In this study, we aim to investigate the types and contents of cytokines in ASC-CM and the effects of some kinds of common cytokines in ASC-CM, such as EGF, PDGF-AA, VEGF, and bFGF, on dermal fibroblasts proliferation and migration in wound healing process. Results showed that these four cytokines had high concentrations in ASC-CM. The migration of skin fibroblasts could be significantly stimulated by VEGF, bFGF, and PDGF-AA, and the proliferation could be significantly stimulated by bFGF and EGF in ASC-CM. Additionally, ASC-CM had more obvious promoting effect on fibroblasts proliferation and migration than single cytokine. These observations suggested that ASC-CM played an important role in the cutaneous injury partly by the synergistic actions of several cytokines in promoting dermal fibroblasts proliferation and migration, and ASC-CM was more adaptive than each single cytokine to be applied in promoting the wound healing.

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