Schwann cell chondroitin sulfate proteoglycan inhibits dorsal root ganglion neuron neurite outgrowth and substrate specificity via a soma and not a growth cone mechanism

2009 ◽  
Vol 87 (13) ◽  
pp. 2863-2871 ◽  
Author(s):  
Damien P. Kuffler ◽  
Ivan J. Sosa ◽  
Onix Reyes
Keyword(s):  
Dorsal Root Ganglion ◽  
Schwann Cell ◽  
Substrate Specificity ◽  
Chondroitin Sulfate ◽  
Neurite Outgrowth ◽  
Dorsal Root Ganglion Neuron ◽  
Growth Cone ◽  
Dorsal Root ◽  
Chondroitin Sulfate Proteoglycan ◽  
Sulfate Proteoglycan

Core Protein of Chondroitin Sulfate Proteoglycan Promotes Neurite Outgrowth from Cultured Neocortical Neurons

1991 ◽  
Vol 56 (2) ◽  
pp. 706-708 ◽  
Author(s):  
Noboru Iijima ◽  
Atsuhiko Oohira ◽  
Toshio Mori ◽  
Katsuaki Kitabatake ◽  
Shinichi Kohsaka
Keyword(s):  
Chondroitin Sulfate ◽  
Neurite Outgrowth ◽  
Core Protein ◽  
Chondroitin Sulfate Proteoglycan ◽  
Sulfate Proteoglycan ◽  
Neocortical Neurons

scholarly journals Studies of adhesion molecules mediating interactions between cells of peripheral nervous system indicate a major role for L1 in mediating sensory neuron growth on Schwann cells in culture.

1988 ◽  
Vol 107 (1) ◽  
pp. 341-351 ◽  
Author(s):  
B Seilheimer ◽  
M Schachner
Keyword(s):  
Nervous System ◽  
Dorsal Root Ganglion ◽  
Schwann Cell ◽  
Neurite Outgrowth ◽  
Adhesion Molecules ◽  
Schwann Cells ◽  
Dorsal Root ◽  
Dorsal Root Ganglion Neurons ◽  
Ganglion Neurons ◽  
Large Neurons

The involvement of the adhesion molecules L1, N-CAM, and J1 in adhesion and neurite outgrowth in the peripheral nervous system was investigated. We prepared Schwann cells and fibroblasts (from sciatic nerves) and neurons (from dorsal root ganglia) from 1-d mice. These cells were allowed to interact with each other in a short-term adhesion assay. We also measured outgrowth of dorsal root ganglion neurons on Schwann cell and fibroblast monolayers. Schwann cells (which express L1, N-CAM, and J1) adhered most strongly to dorsal root ganglion neurons by an L1-dependent mechanism and less by N-CAM and J1. Schwann cell-Schwann cell adhesion was mediated by L1 and N-CAM, but not J1. Adhesion of fibroblasts (which express N-CAM, but not L1 or J1) to neurons or Schwann cells was mediated by L1 and N-CAM and not J1. However, inhibition by L1 and N-CAM antibodies was found to be less pronounced with fibroblasts than with Schwann cells. N-CAM was also strongly involved in fibroblast-fibroblast adhesion. Neurite outgrowth was most extensive on Schwann cells and less on fibroblasts. A difference in extent of neurite elongation was seen between small- (10-20 microns) and large- (20-35 microns) diameter neurons, with the larger neurons tending to exhibit longer neurites. Fab fragments of polyclonal L1, N-CAM, and J1 antibodies exerted slightly different inhibitory effects on neurite outgrowth, depending on whether the neurites were derived from small or large neurons. L1 antibodies interfered most strikingly with neurite outgrowth on Schwann cells (inhibition of 88% for small and 76% for large neurons), while no inhibition was detectable on fibroblasts. Similarly, although to a smaller extent than L1, N-CAM appeared to be involved in neurite outgrowth on Schwann cells and not on fibroblasts. Antibodies to J1 only showed a very small effect on neurite outgrowth of large neurons on Schwann cells. These observations show for the first time that identified adhesion molecules are potent mediators of glia-dependent neurite formation and attribute to L1 a predominant role in neurite outgrowth on Schwann cells which may be instrumental in regeneration.

scholarly journals A neuronal cell surface heparan sulfate proteoglycan is required for dorsal root ganglion neuron stimulation of Schwann cell proliferation.

1985 ◽  
Vol 101 (3) ◽  
pp. 744-754 ◽  
Author(s):  
N Ratner ◽  
R P Bunge ◽  
L Glaser
Keyword(s):  
Dorsal Root Ganglion ◽  
Schwann Cell ◽  
Cell Surface ◽  
Heparan Sulfate ◽  
Schwann Cells ◽  
Dorsal Root ◽  
Dorsal Root Ganglion Neurons ◽  
Sulfate Proteoglycan ◽  
Mitogenic Response ◽  
Ganglion Neurons

Axons of dorsal root ganglion neurons express on their surfaces one or more proteins which are mitogenic for Schwann cells (Salzer, J., R. P. Bunge, and L. Glaser, 1980, J. Cell Biol., 84:767-778). Incubation of co-cultures of dorsal root ganglion neurons and Schwann cells with 4-methylumbelliferyl-beta-D-xyloside, an inhibitor of proteoglycan biosynthesis, decreases the mitogenic response of the Schwann cell by over 95%. The effect of the beta-D-xyloside has been localized to the neurons; pretreatment of neurons but not of Schwann cells with the inhibitor causes a marked reduction of the mitogenic response. In addition, Schwann cells treated with beta-D-xyloside are still mitogenically responsive to soluble Schwann cell mitogens (cholera toxin and glial growth factor). Neurons treated with heparitinase and membrane vesicles prepared from heparitinase-treated neurons show diminished mitogenicity for Schwann cells, while other proteoglycan lyases have no effect. We conclude that a cell surface heparan sulfate proteoglycan is a component of the Schwann cell mitogen present on the surface of dorsal root ganglion neurons.

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