Increase in dopaminergic neurons from mouse embryonic stem cell-derived neural progenitor/stem cells is mediated by hypoxia inducible factor-1α

2008 ◽  
Vol 86 (11) ◽  
pp. 2353-2362 ◽  
Author(s):  
Tae-Sun Kim ◽  
Sachiyo Misumi ◽  
Cha-Gyun Jung ◽  
Tadashi Masuda ◽  
Yoshiaki Isobe ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Dopaminergic Neurons ◽  
Hypoxia Inducible Factor ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Neural Progenitor ◽  
Hypoxia Inducible Factor 1Α

scholarly journals Asymptomatic neurotoxicity of amyloid β-peptides (Aβ1-42 and Aβ25-35) on mouse embryonic stem cell-derived neural cells

2020 ◽  
Author(s):  
Nur Izzati Mansor ◽  
Carolindah Makena Ntimi ◽  
Noraishah Mydin Abdul-Aziz ◽  
King-Hwa Ling ◽  
Aishah Adam ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Neural Cells ◽  
Aβ Peptides ◽  
Intracellular Ros

One of the strategies in the establishment of in vitro oxidative stress models for neurodegenerative diseases, such as Alzheimer’s disease (AD), is to induce neurotoxicity by amyloid beta (Aβ) peptides in suitable neural cells. Presently, data on the neurotoxicity of Aβ in neural cells differentiated from stem cells are limited. In this study, we attempted to induce oxidative stress in transgenic 46C mouse embryonic stem cell-derived neurons via treatment with Ab peptides (Aβ1-42 and Aβ25-35). 46C neural cells were generated by promoting the formation of multicellular aggregates, embryoid bodies (EBs) in the absence of leukemia inhibitory factor (LIF), followed by the addition of all-trans retinoic acid (ATRA) as the neural inducer. Mature neuronal cells were exposed to different concentrations of Aβ1-42 and Aβ25-35 for 24 h. Morphological changes, cell viability, and intracellular ROS production were assessed. We found that 100 µM Aβ1-42 and 50 µM Aβ25-35 only promoted 40% and 10%, respectively, of cell injury and death in the 46C-derived neuronal cells. Interestingly, treatment with each of the Aβ peptides resulted in a significant increase of intracellular ROS activity, as compared to untreated neurons. These findings indicate the potential of using neurons derived from stem cells and Aβ peptides in generating oxidative stress for the establishment of an in vitro AD model that could be useful for drug screening and natural product studies.

Effects of mitogens on proliferation of mouse embryonic stem cell-derived neural stem cells

2010 ◽  
Vol 68 ◽  
pp. e244
Author(s):  
Takuya Yoshie ◽  
Masahiro Otsu ◽  
Hiroyuki Omori ◽  
Masayoshi Shibata ◽  
Risa Ueda ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Neural Stem Cells ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell

scholarly journals Microcircuit formation following transplantation of mouse embryonic stem cell-derived neurons in peripheral nerve

2017 ◽  
Vol 117 (4) ◽  
pp. 1683-1689 ◽  
Author(s):  
Philippe Magown ◽  
Victor F. Rafuse ◽  
Robert M. Brownstone
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem Cells ◽  
Embryonic Stem Cell ◽  
Spontaneous Activity ◽  
Self Assembly ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Muscle Contractions ◽  
Functional Connections

Motoneurons derived from embryonic stem cells can be transplanted in the tibial nerve, where they extend axons to functionally innervate target muscle. Here, we studied spontaneous muscle contractions in these grafts 3 mo following transplantation. One-half of the transplanted grafts generated rhythmic muscle contractions of variable patterns, either spontaneously or in response to brief electrical stimulation. Activity generated by transplanted embryonic stem cell-derived neurons was driven by glutamate and was modulated by muscarinic and GABAergic/glycinergic transmission. Furthermore, rhythmicity was promoted by the same transmitter combination that evokes rhythmic locomotor activity in spinal cord circuits. These results demonstrate that there is a degree of self-assembly of microcircuits in these peripheral grafts involving embryonic stem cell-derived motoneurons and interneurons. Such spontaneous activity is reminiscent of embryonic circuit development in which spontaneous activity is essential for proper connectivity and function and may be necessary for the grafts to form functional connections with muscle. NEW & NOTEWORTHY This manuscript demonstrates that, following peripheral transplantation of neurons derived from embryonic stem cells, the grafts are spontaneously active. The activity is produced and modulated by a number of transmitter systems, indicating that there is a degree of self-assembly of circuits in the grafts.

Intracellular labeling of mouse embryonic stem cell–derived neural progenitor aggregates with micron-sized particles of iron oxide

Cytotherapy ◽  
2015 ◽  
Vol 17 (1) ◽  
pp. 98-111 ◽  
Author(s):  
Sébastien Sart ◽  
Fabian Calixto Bejarano ◽  
Michelle A. Baird ◽  
Yuanwei Yan ◽  
Jens T. Rosenberg ◽  
...  
Keyword(s):  
Stem Cell ◽  
Iron Oxide ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Neural Progenitor

scholarly journals Mu and kappa opioids modulate mouse embryonic stem cell-derived neural progenitor differentiation via MAP kinases

2010 ◽  
Vol 112 (6) ◽  
pp. 1431-1441 ◽  
Author(s):  
Jason W. Hahn ◽  
Shana Jagwani ◽  
Eunhae Kim ◽  
Victoria R. Rendell ◽  
Joy He ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Map Kinases ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Neural Progenitor ◽  
Kappa Opioids

Dynamic Changes of Mouse Embryonic Stem Cell-Derived Neural Stem Cells Under In Vitro Prolonged Culture and Hypoxic Conditions

2019 ◽  
Vol 28 (21) ◽  
pp. 1434-1450 ◽  
Author(s):  
Donny Lukmanto ◽  
Vuong Cat Khanh ◽  
Saori Shirota ◽  
Toshiki Kato ◽  
Mami Matsuo Takasaki ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Neural Stem Cells ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Dynamic Changes ◽  
Hypoxic Conditions ◽  
Prolonged Culture
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