Immune or inflammatory response by the host brain suppresses neuronal differentiation of transplanted ES cell–derived neural precursor cells

2008 ◽  
Vol 86 (9) ◽  
pp. 1936-1943 ◽  
Author(s):  
Makoto Ideguchi ◽  
Mizuya Shinoyama ◽  
Masanori Gomi ◽  
Hideki Hayashi ◽  
Nobuo Hashimoto ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Neuronal Differentiation ◽  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
Es Cell ◽  
Host Brain

scholarly journals Neuronal Differentiation and Extensive Migration of Human Neural Precursor Cells following Co-Culture with Rat Auditory Brainstem Slices

PLoS ONE ◽  
2013 ◽  
Vol 8 (3) ◽  
pp. e57301 ◽  
Author(s):  
Ekaterina Novozhilova ◽  
Petri Olivius ◽  
Piyaporn Siratirakun ◽  
Cecilia Lundberg ◽  
Ulrica Englund-Johansson
Keyword(s):  
Neuronal Differentiation ◽  
Auditory Brainstem ◽  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
Brainstem Slices

scholarly journals Stabilized β-Catenin Functions through TCF/LEF Proteins and the Notch/RBP-Jκ Complex To Promote Proliferation and Suppress Differentiation of Neural Precursor Cells

2008 ◽  
Vol 28 (24) ◽  
pp. 7427-7441 ◽  
Author(s):  
Takeshi Shimizu ◽  
Tetsushi Kagawa ◽  
Toshihiro Inoue ◽  
Aya Nonaka ◽  
Shinji Takada ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Neuronal Differentiation ◽  
Glycogen Synthase ◽  
Precursor Cells ◽  
Precursor Cell ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
Self Renewal ◽  
Fgf Receptor ◽  
Proliferation And Differentiation

ABSTRACT The proliferation and differentiation of neural precursor cells are mutually exclusive during brain development. Despite its importance for precursor cell self renewal, the molecular linkage between these two events has remained unclear. Fibroblast growth factor 2 (FGF2) promotes neural precursor cell proliferation and concurrently inhibits their differentiation, suggesting a cross talk between proliferation and differentiation signaling pathways downstream of the FGF receptor. We demonstrate that FGF2 signaling through phosphatidylinositol 3 kinase activation inactivates glycogen synthase kinase 3β (GSK3β) and leads to the accumulation of β-catenin in a manner different from that in the Wnt canonical pathway. The nuclear accumulated β-catenin leads to cell proliferation by activating LEF/TCF transcription factors and concurrently inhibits neuronal differentiation by potentiating the Notch1-RBP-Jκ signaling pathway. β-Catenin and the Notch1 intracellular domain form a molecular complex with the promoter region of the antineurogenic hes1 gene, allowing its expression. This signaling interplay is especially essential for neural stem cell maintenance, since the misexpression of dominant-active GSK3β completely inhibits the self renewal of neurosphere-forming stem cells and prompts their neuronal differentiation. Thus, the GSK3β/β-catenin signaling axis regulated by FGF and Wnt signals plays a pivotal role in the maintenance of neural stem/precursor cells by linking the cell proliferation to the inhibition of differentiation.

scholarly journals Analysis of Gene Expression in Mouse Spinal Cord-derived Neural Precursor Cells During Neuronal Differentiation

2009 ◽  
Vol 7 (2) ◽  
pp. 85-96
Author(s):  
Joon-Ik Ahn ◽  
So-Young Kim ◽  
Moon-Jeong Ko ◽  
Hye-Joo Chung ◽  
Ho-Sang Jeong
Keyword(s):  
Gene Expression ◽  
Spinal Cord ◽  
Neuronal Differentiation ◽  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
Mouse Spinal Cord

scholarly journals BDNF Increases Survival and Neuronal Differentiation of Human Neural Precursor Cells Cotransplanted with a Nanofiber Gel to the Auditory Nerve in a Rat Model of Neuronal Damage

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Yu Jiao ◽  
Björn Palmgren ◽  
Ekaterina Novozhilova ◽  
Ulrica Englund Johansson ◽  
Anne L. Spieles-Engemann ◽  
...  
Keyword(s):  
Neuronal Differentiation ◽  
Auditory Nerve ◽  
Neuronal Damage ◽  
Round Window ◽  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
Phenotypic Differentiation ◽  
Round Window Niche ◽  
The Brain

Objectives. To study possible nerve regeneration of a damaged auditory nerve by the use of stem cell transplantation.Methods. We transplanted HNPCs to the rat AN trunk by the internal auditory meatus (IAM). Furthermore, we studied if addition of BDNF affects survival and phenotypic differentiation of the grafted HNPCs. A bioactive nanofiber gel (PA gel), in selected groups mixed with BDNF, was applied close to the implanted cells. Before transplantation, all rats had been deafened by a round window niche application ofβ-bungarotoxin. This neurotoxin causes a selective toxic destruction of the AN while keeping the hair cells intact.Results. Overall, HNPCs survived well for up to six weeks in all groups. However, transplants receiving the BDNF-containing PA gel demonstrated significantly higher numbers of HNPCs and neuronal differentiation. At six weeks, a majority of the HNPCs had migrated into the brain stem and differentiated. Differentiated human cells as well as neurites were observed in the vicinity of the cochlear nucleus.Conclusion. Our results indicate that human neural precursor cells (HNPC) integration with host tissue benefits from additional brain derived neurotrophic factor (BDNF) treatment and that these cells appear to be good candidates for further regenerative studies on the auditory nerve (AN).

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