Adult human spinal cord harbors neural precursor cells that generate neurons and glial cells in vitro

2008 ◽  
Vol 86 (9) ◽  
pp. 1916-1926 ◽  
Author(s):  
C. Dromard ◽  
H. Guillon ◽  
V. Rigau ◽  
C. Ripoll ◽  
J.C. Sabourin ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Glial Cells ◽  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
Human Spinal Cord ◽  
Adult Human

173. Neural Precursor Cells Produce Trophic Factors In Vitro and Following In Vivo Transplantation into the Injured Spinal Cord

2008 ◽  
Vol 8 (5) ◽  
pp. 88S
Author(s):  
Gregory Hawryluk ◽  
Eftekhar Eftekharpour ◽  
Soheila Karimi-Abdolrezaee ◽  
Michael Fehlings
Keyword(s):  
Spinal Cord ◽  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Trophic Factors ◽  
Neural Precursor ◽  
Injured Spinal Cord

Transplantation of Clonal Neural Precursor Cells Derived from Adult Human Brain Establishes Functional Peripheral Myelin in the Rat Spinal Cord

2001 ◽  
Vol 167 (1) ◽  
pp. 27-39 ◽  
Author(s):  
Yukinori Akiyama ◽  
Osamu Honmou ◽  
Takaaki Kato ◽  
Teiji Uede ◽  
Kazuo Hashi ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Human Brain ◽  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
Rat Spinal Cord ◽  
Adult Human Brain ◽  
Adult Human

In vitro differentiation of human bone marrow stromal cells into neural precursor cells using small molecules

2021 ◽  
Vol 363 ◽  
pp. 109340
Author(s):  
Abeer Sallam ◽  
Thangirala Sudha ◽  
Noureldien H.E. Darwish ◽  
Samar Eghotny ◽  
Abeer E-Dief ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Small Molecules ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Precursor Cells ◽  
Marrow Stromal Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
In Vitro Differentiation

Comparison of the therapeutic potential of adult and embryonic neural precursor cells in a rat model of Parkinson disease

2008 ◽  
Vol 108 (1) ◽  
pp. 149-159 ◽  
Author(s):  
Kenichiro Muraoka ◽  
Tetsuro Shingo ◽  
Takao Yasuhara ◽  
Masahiro Kameda ◽  
Wen Ji Yuen ◽  
...  
Keyword(s):  
Parkinson Disease ◽  
Rat Model ◽  
Fluorescent Protein ◽  
Therapeutic Potential ◽  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
Therapeutic Effects ◽  
Adenoviral Infection

Object The therapeutic effects of adult and embryonic neural precursor cells (NPCs) were evaluated and their therapeutic potential compared in a rat model of Parkinson disease. Methods Adult NPCs were obtained from the subventricular zone and embryonic NPCs were taken from the ganglionic eminence of 14-day-old embryos. Each NPC type was cultured with epidermal growth factor. The in vitro neuronal differentiation rate of adult NPCs was approximately equivalent to that of embryonic NPCs after two passages. Next, the NPCs were transfected with either green fluorescent protein or glial cell line–derived neurotrophic factor (GDNF) by adenoviral infection and transplanted into the striata in a rat model of Parkinson disease (PD) induced by unilateral intrastriatal injection of 6-hydroxydopamine. An amphetamine-induced rotation test was used to evaluate rat behavioral improvement, and immunohistochemical analysis was performed to compare grafted cell survival, differentiation, and host tissue changes. Results The rats with GDNF-transfected NPCs had significantly fewer amphetamine-induced rotations and less histological damage. Except for the proportion of surviving grafted cells, there were no significant differences between adult and embryonic NPCs. Conclusions Adult and embryonic NPCs have a comparable therapeutic potential in a rat model of PD.

scholarly journals Prolonged cultivation of hippocampal neural precursor cells shifts their differentiation potential and selects for aneuploid cells

2013 ◽  
Vol 394 (12) ◽  
pp. 1623-1636 ◽  
Author(s):  
The Duy Nguyen ◽  
Darius Widera ◽  
Johannes Greiner ◽  
Janine Müller ◽  
Ina Martin ◽  
...  
Keyword(s):  
Cell Types ◽  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Neural Precursor ◽  
Early Passage ◽  
Differentiation Potential ◽  
Developmental Potential ◽  
Prolonged Cultivation ◽  
Tumorigenic Transformation

Abstract Neural precursor cells (NPCs) are lineage-restricted neural stem cells with limited self-renewal, giving rise to a broad range of neural cell types such as neurons, astrocytes, and oligodendrocytes. Despite this developmental potential, the differentiation capacity of NPCs has been controversially discussed concerning the trespassing lineage boundaries, for instance resulting in hematopoietic competence. Assessing their in vitro plasticity, we isolated nestin+/Sox2+, NPCs from the adult murine hippocampus. In vitro-expanded adult NPCs were able to form neurospheres, self-renew, and differentiate into neuronal, astrocytic, and oligodendrocytic cells. Although NPCs cultivated in early passage efficiently gave rise to neuronal cells in a directed differentiation assay, extensively cultivated NPCs revealed reduced potential for ectodermal differentiation. We further observed successful differentiation of long-term cultured NPCs into osteogenic and adipogenic cell types, suggesting that NPCs underwent a fate switch during culture. NPCs cultivated for more than 12 passages were aneuploid (abnormal chromosome numbers such as 70 chromosomes). Furthermore, they showed growth factor-independent proliferation, a hallmark of tumorigenic transformation. In conclusion, our findings substantiate the lineage restriction of NPCs from adult mammalian hippocampus. Prolonged cultivation results, however, in enhanced differentiation potential, which may be attributed to transformation events leading to aneuploid cells.

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