Neuronal differentiation elicited by glial cell line-derived neurotrophic factor and ciliary neurotrophic factor in adrenal chromaffin cell line tsAM5D immortalized with temperature-sensitive SV40 T-antigen

2008 ◽  
Vol 86 (8) ◽  
pp. 1694-1710 ◽  
Author(s):  
Tomiyasu Murata ◽  
Masaru Tsuboi ◽  
Naoshi Koide ◽  
Kiyomi Hikita ◽  
Susumu Kohno ◽  
...  
Keyword(s):  
Cell Line ◽  
Neuronal Differentiation ◽  
Neurotrophic Factor ◽  
Chromaffin Cell ◽  
Ciliary Neurotrophic Factor ◽  
T Antigen ◽  
Temperature Sensitive ◽  
Sv40 T Antigen ◽  
Adrenal Chromaffin Cell ◽  
Adrenal Chromaffin

A novel stromal cell-dependent hematopoietic cell line established from temperature-sensitive SV40 T-antigen transgenic mice

1999 ◽  
Vol 27 (6) ◽  
pp. 1087-1096 ◽  
Author(s):  
Nobuaki Yanai ◽  
Naoko Matsui ◽  
Ken-Ichi Matsuda ◽  
Tadashi Furusawa ◽  
Tadashi Okubo ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Cell Line ◽  
Stromal Cell ◽  
T Antigen ◽  
Hematopoietic Cell ◽  
Temperature Sensitive ◽  
Sv40 T Antigen ◽  
Hematopoietic Cell Line

A diacylglycerol-activated Ca2+ channel in PC12 cells (an adrenal chromaffin cell line) correlates with expression of the TRP-6 (transient receptor potential) protein

2001 ◽  
Vol 358 (3) ◽  
pp. 717-726 ◽  
Author(s):  
Yordanos TESFAI ◽  
Helen M. BRERETON ◽  
Greg J. BARRITT
Keyword(s):  
Cell Line ◽  
Pc12 Cells ◽  
Chromaffin Cell ◽  
Transient Receptor Potential ◽  
Receptor Potential ◽  
Flufenamic Acid ◽  
Animal Cells ◽  
Adrenal Chromaffin Cell ◽  
Transient Receptor ◽  
Adrenal Chromaffin

The structures, and mechanisms of activation, of plasma membrane intracellular-messenger-activated, non-selective cation channels in animal cells are not well understood. The PC12 adrenal chromaffin cell line is a well-characterized example of a nerve cell. In PC12 cells, 1-oleolyl-2-acetyl-sn-glycerol (OAG), a membrane-permeant analogue of diacylglycerol, initiated the inflow of Ca2+, Mn2+ and Sr2+. Acetylcholine and thapsigargin initiated the inflow of Ca2+ and Mn2+, but not of Sr2+. The activation of bivalent cation inflow by OAG: (i) was mimicked by another membrane-permeant diacylglycerol analogue, 1,2-dioctanoyl-sn-glycerol, but not by the membrane-impermeant analogue 1-stearoyl-2-arachidonyl-sn-glycerol; (ii) was not blocked by staurosporin or chelerythrine, inhibitors of protein kinase C; (iii) was enhanced by RHC80267 and R50922, inhibitors of diacylglycerol lipase and diacylglycerol kinase respectively; and (iv) was inhibited by extracellular Ca2+. When OAG was added after acetylcholine, the effect of OAG on Ca2+ inflow was over-and-above that induced by acetylcholine. 2-Aminoethyl diphenylborate (2-APB) inhibited Ca2+ inflow initiated by either acetylcholine or thapsigargin, but not that initiated by OAG. Flufenamic acid inhibited OAG-initiated, but not acetylcholine-initiated, Ca2+ and Mn2+ inflow. OAG-initiated Ca2+ inflow was less sensitive to inhibition by SK&F96365 than acetylcholine-initiated Ca2+ inflow. In polyadenylated RNA prepared from PC12 cells, mRNA encoding TRP (transient receptor potential) proteins 1–6 was detected by reverse transcriptase (RT)-PCR, and in lysates of PC12 cells the endogenous TRP-6 protein was detected by Western blot analysis. It is concluded that PC12 cells express a diacylglycerol-activated, non-selective cation channel. Expression of this channel function correlates with expression of the TRP-3 and TRP-6 proteins, which have been shown previously to be activated by diacylglycerol when expressed heterologously in animal cells [Hofmann, Obukhov, Schaefer, Harteneck, Gudermann, and Schultz (1999) Nature (London) 397, 259–263].

Establishment of a Human Retinal Cell Line by Transfection of SV40 T Antigen Gene with Potential to Undergo Neuronal Differentiation

1994 ◽  
Vol 13 (9) ◽  
pp. 909-921 ◽  
Author(s):  
KAMLA DUTT ◽  
MATTIE SCOTT ◽  
MIER WANG ◽  
EUGENE SEMPLE ◽  
GANPATI P. SHARMA ◽  
...  
Keyword(s):  
Cell Line ◽  
Neuronal Differentiation ◽  
T Antigen ◽  
Retinal Cell ◽  
Sv40 T Antigen ◽  
Antigen Gene
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