Neural differentiation and potential use of stem cells from the human umbilical cord for central nervous system transplantation therapy

2008 ◽  
Vol 86 (8) ◽  
pp. 1670-1679 ◽  
Author(s):  
Choon Bing Low ◽  
Yih-Cherng Liou ◽  
Bor Luen Tang
Keyword(s):  
Stem Cells ◽  
Central Nervous System ◽  
Nervous System ◽  
Umbilical Cord ◽  
Neural Differentiation ◽  
Human Umbilical Cord ◽  
Potential Use ◽  
Transplantation Therapy

Applications of Human Umbilical Cord Blood Cells in Central Nervous System Regeneration

2010 ◽  
Vol 5 (1) ◽  
pp. 17-22 ◽  
Author(s):  
Antonio Herranz ◽  
Rafael Gonzalo-Gobernado ◽  
Diana Reimers ◽  
Maria Asensio ◽  
Macarena Rodriguez-Serrano ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Blood Cells ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord ◽  
Cord Blood Cells ◽  
Umbilical Cord Blood Cells

Human umbilical cord blood (HUCB) cells for central nervous system repair

2003 ◽  
Vol 5 (5) ◽  
pp. 355-368 ◽  
Author(s):  
Mary B. Newman ◽  
Cyndy D. Davis ◽  
Nicole Kuzmin-Nichols ◽  
Paul R. Sanberg
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord ◽  
Central Nervous System Repair

scholarly journals Effects of Serial Passage on the Characteristics and Cardiac and Neural Differentiation of Human Umbilical Cord Wharton’s Jelly-Derived Mesenchymal Stem Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Jianchun Lian ◽  
Shijie Lv ◽  
Chang Liu ◽  
Yang Liu ◽  
Shujun Wang ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Cell Proliferation ◽  
Umbilical Cord ◽  
Neural Differentiation ◽  
Serial Passage ◽  
Wharton’S Jelly ◽  
Human Umbilical Cord ◽  
Wharton's Jelly ◽  
Marker Expression

Background and Objective. It is important to guarantee the quality of stem cells. Serial passage is the main approach to expand stem cells. This study evaluated effects of serial passage on the biological characteristics of human umbilical cord Wharton’s jelly-derived MSCs (WJ MSCs).Methods. Biological properties of WJ MSCs in the early (less than 10 passages, P10), middle (P11–20), and late (more than P20) phases including cell proliferation, cell cycle, phenotype, senescence, oncogene expression, stemness marker expression, and differentiation capacity were evaluated using flow cytometry, real-time PCR, immunocytofluorescence, and western blot.Results. It was found that there were no significant differences in cell proliferation, cell cycle, phenotype, and stemness marker expression in different phases. However, the expression of senescence-related gene, p21, and oncogene, c-Myc, was significantly upregulated in the late phase, which had close relations with the obviously increased cell senescence. Moreover, cardiac differentiation capability of WJ MSCs decreased whereas the propensity for neural differentiation increased significantly in the middle phase.Conclusions. This study reveals that WJ MSCs in the early and middle phases are relatively stable, and effect of serial passage on the lineage-specific differentiation should be considered carefully.

Human Umbilical Cord Mesenchymal Stem Cells Efficiently Improve Neurobehavioral Status and Alleviate Brain Injury in Hypoxia/Ischemia-Induced Cerebral Palsy Rat Model Via Down-Regulating the Nogoa/Ngr/Rho Pathway

2021 ◽  
Author(s):  
Yaoling Luo ◽  
Zhengyi He ◽  
Minhong Zhang ◽  
Zhengwei Zou ◽  
Lincai Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Nervous System ◽  
Mesenchymal Stem Cells ◽  
Cerebral Palsy ◽  
Brain Injury ◽  
Umbilical Cord ◽  
Rat Model ◽  
Human Umbilical Cord ◽  
Hypoxia Ischemia ◽  
Brain Tissues

Abstract Background: Cerebral palsy (CP) is a brain injury disease, which is a global public health issue with an estimated prevalence of 2‰—4‰ and imposes a substantial health burden on many countries. At present, there is no ideal treatment available and most of them will still suffer adverse outcomes. Human umbilical cord mesenchymal stem cells(HUCMSCs) application in many fields of medicine, which can promote nervous system regeneration and inhibit neuroinflammation. The regeneration of central nervous system(CNS) is related to the nervous regeneration inhibitors. NogoA/NgR/Rho pathway is very important to the nerve growth, CP injury is inevitably accompanied by the regeneration and repair of neurons and axons. so we hypothesized that NogoA/NgR/Rho pathway is involved in when using the HUCMSCs to treatment cerebral palsy.Purpose: In this study, we might clarify the NogoA/NgR/Rho pathway functional role in mediating HUCMSCs to improve neurobehavioral status and alleviate brain injury in hypoxia/ischemia-induced CP rat model.Methods: The CP rat model was established by ligating the left common carotid artery and anoxia for 2.5 h, and HUCMSCs were intravenous injected to the modeled rats. The neurobehavioral situation and brain pathological injury in CP rats were determined via a series of assays. The mRNA and protein expression of NogoA、NgR、RhoA、Rac-1、Cdc42 in brain tissue of rats in each group was detected by RT-qPCR and western blot analysis. Results: The CP rats exhibited obvious motor function abnormalities, pathological damage and a lot of brain nerve cell apoptosis. Compared with CP+PBS group and CP group rats, HUCMSCs transplantation can significantly improve the neurobehavioral situation, attenuated brain pathological injury, inhibit apoptosis of brain nerve cells and the activation of astrocytes in CP rats. The expression of NogoA、NgR、RhoA relative mRNA and protein in brain tissues of rats in the CP+PBS group and CP group rats were significantly lower than those of in the sham+PBS and CP+HUCMSCs group. The expression of Rac-1、Cdc42 relative mRNA and protein in brain tissues of rats in the sham and CP+HUCMSCs group was significantly higher than those of in CP+PBS group and CP group rats. Conclusion: This study confirmed that HUCMSCs can efficiently improve neurobehavioral status and alleviate brain injury in hypoxia/ischemia-induced cerebral palsy rat model via down-regulating the NogoA/NgR/Rho pathway.

Effect of photobiomodulation on neural differentiation of human umbilical cord mesenchymal stem cells

2018 ◽  
Vol 34 (4) ◽  
pp. 667-675 ◽  
Author(s):  
Hongli Chen ◽  
Hongjun Wu ◽  
Huijuan Yin ◽  
Jinhai Wang ◽  
Huajiang Dong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Neural Differentiation ◽  
Human Umbilical Cord
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