Neuregulin isoforms in dorsal root ganglion neurons: Effects of the cytoplasmic domain on localization and membrane shedding of Nrg-1 type I

2006 ◽  
Vol 84 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Zhiyou Zhang ◽  
Lisa Prentiss ◽  
Deborah Heitzman ◽  
Richard C. Stahl ◽  
Frank DiPino ◽  
...  
Keyword(s):  
Dorsal Root Ganglion ◽  
Dorsal Root ◽  
Cytoplasmic Domain ◽  
Dorsal Root Ganglion Neurons ◽  
Type I ◽  
Ganglion Neurons ◽  
Membrane Shedding

scholarly journals A New Method for Primary Culture of Mouse Dorsal Root Ganglion Neurons

2018 ◽  
Author(s):  
Tiantian Dong ◽  
Shigang Li ◽  
Wei Liu ◽  
Mengzhen Yan ◽  
Jie Yu ◽  
...  
Keyword(s):  
Dorsal Root Ganglion ◽  
Primary Culture ◽  
Dorsal Root ◽  
Neuron Specific Enolase ◽  
Dorsal Root Ganglion Neurons ◽  
Immunocytochemical Staining ◽  
Type I ◽  
Reliable Model ◽  
Mouse Dorsal Root Ganglion ◽  
Ganglion Neurons

AbstractIn order to establish a simple and highly purified method for primary culture of mouse dorsal root ganglion neurons(DRGn), in this study, the DRGn of young mice were obtained by collagenase type I and trypsin digestion. Then the DRGn were obtained by immunocytochemical staining of mouse neuron specific enolase (NSE) monoclonal antibody, while using flow cytometry to further detect the positive rates of DRGn. The cultured primary DRGn grew well and had a purity of about 83.72%. The DRGn survival time was 60 days when cultured in DMEM medium containing nerve growth factor (NGF). The culture scheme is simple and stable, and a large number of high purity DRGn could be cultured, which provides a reliable model for further study of nerve cells.

Somatostatin Type 2 Receptor Antibody Enhances Mechanical Hyperalgesia in the Dorsal Root Ganglion Neurons after Sciatic Nerve-pinch Injury: Evidence of Behavioral Studies and Bax Protein Expression

2020 ◽  
Vol 18 (10) ◽  
pp. 791-797
Author(s):  
Qiong Xiang ◽  
Jing-Jing Li ◽  
Chun-Yan Li ◽  
Rong-Bo Tian ◽  
Xian-Hui Li
Keyword(s):  
Sciatic Nerve ◽  
Dorsal Root Ganglion ◽  
Nerve Injury ◽  
Dorsal Root ◽  
Underlying Mechanism ◽  
Dorsal Root Ganglion Neurons ◽  
Mechanical Hyperalgesia ◽  
Bax Protein ◽  
Ganglion Neurons

Background: Our previous study has indicated that somatostatin potently inhibits neuropathic pain through the activation of its type 2 receptor (SSTR2) in mouse dorsal root ganglion and spinal cord. However, the underlying mechanism of this activation has not been elucidated clearly Objective: The aim of this study is to perform the pharmacological studies on the basis of sciatic nerve-pinch mice model and explore the underlying mechanism involving SSTR2. Methods: On the basis of a sciatic nerve-pinch injury model, we aimed at comparing the painful behavior and dorsal root ganglion neurons neurochemical changes after the SSTR2 antibody (anti- SSTR2;5μl,1μg/ml) administration in the mouse. Results: After pinch nerve injury, we found that the mechanical hyperalgesia and severely painful behavior (autotomy) were detected after the application of SSTR2 antibody (anti-SSTR2; 5μl, 1μg/ml) on the pinch-injured nerve. The up-regulated phosphorylated ERK (p-ERK) expression and the apoptotic marker (i.e., Bax) were significantly decreased in DRGs after anti-SSTR2 treatment. Conclusion: The current data suggested that inhibitory changes in proteins from the apoptotic pathway in anti-SSTR2-treated groups might be taking place to overcome the protein deficits caused by SSTR2 antibody and supported the new therapeutic intervention with SSTR2 antagonist for neuronal degeneration following nerve injury.

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