Functional reconstitution of ?-secretase through coordinated expression of presenilin, nicastrin, Aph-1, and Pen-2

2004 ◽  
Vol 77 (3) ◽  
pp. 309-322 ◽  
Author(s):  
Goran Periz ◽  
Mark E. Fortini
Keyword(s):  
Coordinated Expression

scholarly journals Xenorhabdus nematophila Requires an Intact iscRSUA-hscBA-fdx Operon To Colonize Steinernema carpocapsae Nematodes

2003 ◽  
Vol 185 (12) ◽  
pp. 3678-3682 ◽  
Author(s):  
Eric C. Martens ◽  
Joseph Gawronski-Salerno ◽  
Danielle L. Vokal ◽  
Molly C. Pellitteri ◽  
Megan L. Menard ◽  
...  
Keyword(s):  
Steinernema Carpocapsae ◽  
Xenorhabdus Nematophila ◽  
Coordinated Expression

ABSTRACT An insertion between iscA and hscB of the Xenorhabdus nematophila iscRSUA-hscBA-fdx locus, predicted to encode Fe-S assembly machinery, prevented colonization of Steinernema carpocapsae nematodes. The insertion disrupted cotranscription of iscA and hscB, but did not reduce hscBA expression, suggesting that X. nematophila requires coordinated expression of the isc-hsc-fdx locus for colonization.

scholarly journals The Coordinated Upregulated Expression of Genes Involved in MEP, Chlorophyll, Carotenoid and Tocopherol Pathways, Mirrored the Corresponding Metabolite Contents in Rice Leaves during De-Etiolation

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1456
Author(s):  
Xin Jin ◽  
Can Baysal ◽  
Margit Drapal ◽  
Yanmin Sheng ◽  
Xin Huang ◽  
...  
Keyword(s):  
Higher Plants ◽  
Expression Patterns ◽  
Regulatory Elements ◽  
Isoprenoid Biosynthesis ◽  
Promoter Regions ◽  
Expression Of Genes ◽  
Coordinated Expression ◽  
Rice Leaves ◽  
Mechanistic Basis ◽  
Phosphate Synthase

Light is an essential regulator of many developmental processes in higher plants. We investigated the effect of 4-hydroxy-3-methylbut-2-enyl diphosphate reductase 1/2 genes (OsHDR1/2) and isopentenyl diphosphate isomerase 1/2 genes (OsIPPI1/2) on the biosynthesis of chlorophylls, carotenoids, and phytosterols in 14-day-old etiolated rice (Oyza sativa L.) leaves during de-etiolation. However, little is known about the effect of isoprenoid biosynthesis genes on the corresponding metabolites during the de-etiolation of etiolated rice leaves. The results showed that the levels of α-tocopherol were significantly increased in de-etiolated rice leaves. Similar to 1-deoxy-D-xylulose-5-phosphate synthase 3 gene (OsDXS3), both OsDXS1 and OsDXS2 genes encode functional 1-deoxy-D-xylulose-5-phosphate synthase (DXS) activities. Their expression patterns and the synthesis of chlorophyll, carotenoid, and tocopherol metabolites suggested that OsDXS1 is responsible for the biosynthesis of plastidial isoprenoids in de-etiolated rice leaves. The expression analysis of isoprenoid biosynthesis genes revealed that the coordinated expression of the MEP (2-C-methyl-D-erythritol 4-phosphate) pathway, chlorophyll, carotenoid, and tocopherol pathway genes mirrored the changes in the levels of the corresponding metabolites during de-etiolation. The underpinning mechanistic basis of coordinated light-upregulated gene expression was elucidated during the de-etiolation process, specifically the role of light-responsive cis-regulatory motifs in the promoter region of these genes. In silico promoter analysis showed that the light-responsive cis-regulatory elements presented in all the promoter regions of each light-upregulated gene, providing an important link between observed phenotype during de-etiolation and the molecular machinery controlling expression of these genes.

scholarly journals Assembly of Two Transgenes in an Artificial Chromatin Domain Gives Highly Coordinated Expression in Tobacco

Genetics ◽  
2002 ◽  
Vol 160 (2) ◽  
pp. 727-740
Author(s):  
Ludmila Mlynárová ◽  
Annelies Loonen ◽  
Elzbieta Mietkiewska ◽  
Ritsert C Jansen ◽  
Jan-Peter Nap
Keyword(s):  
Enzyme Activities ◽  
Firefly Luciferase ◽  
Chromatin Domain ◽  
Mrna Levels ◽  
Loop Model ◽  
Data Generation ◽  
Genome Data ◽  
The Matrix ◽  
Coordinated Expression ◽  
Firefly Luciferase Gene

Abstract The chromatin loop model predicts that genes within the same chromatin domain exhibit coordinated regulation. We here present the first direct experimental support for this model in plants. Two reporter genes, the E. coli β-glucuronidase gene and the firefly luciferase gene, driven by different promoters, were placed between copies of the chicken lysozyme A element, a member of the matrix-associated region (MAR) group of chromatin boundary elements, and introduced in tobacco (Nicotiana tabacum). In plants carrying A elements, quantitative enzyme activities and mRNA levels of both genes show high correlations compared to control plants. The A element thus creates an artificial chromatin domain that yields coordinated expression. Surprisingly, enzyme activities correlated poorly with their respective mRNA levels. We hypothesize that this indicates the occurrence of “error pipelines” in data generation: systematic errors of a given analytical method will point in the same direction and cancel out in correlation analysis, resulting in better correlations. In combining different methods of analysis, however, such errors do not cancel out and as a result relevant correlations can be masked. Such error pipelines will have to be taken into account when different types of (e.g., whole-genome) data sets are combined in quantitative analyses.

scholarly journals Inducible nitric oxide synthase inhibits oxygen consumption in collateral-dependent myocardium

2014 ◽  
Vol 306 (3) ◽  
pp. H356-H362 ◽  
Author(s):  
Yingjie Chen ◽  
Ping Zhang ◽  
Jingxin Li ◽  
Xin Xu ◽  
Robert J. Bache
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Vascular Endothelial Cells ◽  
Coronary Artery Occlusion ◽  
Artery Occlusion ◽  
Coordinated Expression ◽  
Collateral Vessels ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

Following coronary artery occlusion growth of collateral vessels can provide an effective blood supply to the dependent myocardium. The ischemia, which results in growth of collateral vessels, recruits an inflammatory response with expression of cytokines and growth factors, upregulation of endothelial nitric oxide (NO) synthase (eNOS) in vascular endothelial cells, and expression of inducible nitric oxide synthase (iNOS) in both vessels and cardiac myocytes. Because NO is a potent collateral vessel dilator, this study examined whether NO derived from iNOS or constitutive NOS regulates myocardial blood flow (MBF) in the collateral region. Nonselective NOS inhibition with NG-nitro-l-arginine (LNA) caused vasoconstriction with a significant decrease in MBF to the collateral region during exercise. In contrast, the highly selective iNOS inhibitor 1400W caused a 21 ± 5% increase of MBF in the collateral region. This increase in MBF following selective iNOS blockade was proportionate to an increase in myocardial O2 consumption (MV̇o2). The results suggest that NO produced by iNOS inhibits MV̇o2 in the collateralized region, so that the increase in MBF following iNOS blockade was the result of metabolic vasodilation secondary to an increase in MV̇o2. Thus the coordinated expression of iNOS to restrain MV̇o2 and eNOS to maintain collateral vasodilation act to optimize the O2 supply-demand relationship and protect the collateralized myocardium from ischemia.

COEXPRESSIONFINDER: A NEW ALGORITHM FOR FINDING GROUPS OF COEXPRESSED GENES

2006 ◽  
Vol 04 (04) ◽  
pp. 853-864
Author(s):  
SERGEY I. ROGOV ◽  
KUVAT T. MOMYNALIEV ◽  
VADIM M. GOVORUN
Keyword(s):  
Gene Expression ◽  
Hierarchical Clustering ◽  
Parallel Execution ◽  
Expression Vectors ◽  
Dna Arrays ◽  
Java Application ◽  
Coordinated Expression ◽  
Series Of Experiments ◽  
Coexpressed Genes

Results: A new algorithm is developed which is intended to find groups of genes whose expression values change in a concordant manner in a series of experiments with DNA arrays. This algorithm is named as CoexpressionFinder. It can find more complete and internally coordinated groups of gene expression vectors than hierarchical clustering. Also, it finds more genes having coordinated expression. The algorithm's design allows parallel execution. Availability: The algorithm is implemented as a Java application which is freely available at: and .

scholarly journals Proteomic analysis of haem-binding protein from Arabidopsis thaliana and Cyanidioschyzon merolae

2020 ◽  
Vol 375 (1801) ◽  
pp. 20190488 ◽  
Author(s):  
Takayuki Shimizu ◽  
Rintaro Yasuda ◽  
Yui Mukai ◽  
Ryo Tanoue ◽  
Tomohiro Shimada ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Proteomic Analysis ◽  
High Performance ◽  
Biochemical Analysis ◽  
Cyanidioschyzon Merolae ◽  
Genes Expression ◽  
Retrograde Signalling ◽  
Coordinated Expression ◽  
Retrograde Signal ◽  
Affinity Beads

Chloroplast biogenesis involves the coordinated expression of the plastid and nuclear genomes, requiring information to be sent from the nucleus to the developing chloroplasts and vice versa. Although it is well known how the nucleus controls chloroplast development, it is still poorly understood how the plastid communicates with the nucleus. Currently, haem is proposed as a plastid-to-nucleus (retrograde) signal that is involved in various physiological regulations, such as photosynthesis-associated nuclear genes expression and cell cycle in plants and algae. However, components that transduce haem-dependent signalling are still unidentified. In this study, by using haem-immobilized high-performance affinity beads, we performed proteomic analysis of haem-binding proteins from Arabidopsis thaliana and Cyanidioschyzon merolae . Most of the identified proteins were non-canonical haemoproteins localized in various organelles. Interestingly, half of the identified proteins were nucleus proteins, some of them have a similar function or localization in either or both organisms. Following biochemical analysis of selective proteins demonstrated haem binding. This study firstly demonstrates that nucleus proteins in plant and algae show haem-binding properties. This article is part of the theme issue ‘Retrograde signalling from endosymbiotic organelles’.

scholarly journals Spread and survival of promiscuous IncP-1 plasmids.

2003 ◽  
Vol 50 (2) ◽  
pp. 425-453 ◽  
Author(s):  
Małgorzata Adamczyk ◽  
Grazyna Jagura-Burdzy
Keyword(s):  
Unique Feature ◽  
Central Control ◽  
Conjugative Transfer ◽  
Incompatibility Group ◽  
Global Regulators ◽  
Current State ◽  
Expression Of Genes ◽  
Low Copy Number ◽  
Coordinated Expression ◽  
Stable Maintenance

Plasmids classified to the IncP-1 incompatibility group belong to the most stably maintained mobile elements among low copy number plasmids known to date. The remarkable persistence is achieved by various tightly controlled stability mechanisms like active partitioning, efficient conjugative transfer system, killing of plasmid-free segregants and multimer resolution. The unique feature of IncP-1 plasmids is the central control operon coding for global regulators which control the expression of genes involved in vegetative replication, stable maintenance and conjugative transfer. The multivalent regulatory network provides means for coordinated expression of all plasmid functions. The current state of knowledge about two fully sequenced plasmids RK2 and R751, representatives of the IncP-1alpha and IncP-1beta subgroups, is presented.

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