Comparative study of cell culture and purification methods to obtain highly enriched cultures of proliferating adult rat Schwann cells

2004 ◽  
Vol 77 (3) ◽  
pp. 453-461 ◽  
Author(s):  
Christina Mauritz ◽  
Claudia Grothe ◽  
Kirsten Haastert
Keyword(s):  
Cell Culture ◽  
Comparative Study ◽  
Schwann Cells ◽  
Adult Rat ◽  
Purification Methods ◽  
Enriched Cultures

Novel method to obtain highly enriched cultures of adult rat Schwann cells

2010 ◽  
Vol 32 (6) ◽  
pp. 781-786 ◽  
Author(s):  
Ali Niapour ◽  
Fereshteh Karamali ◽  
Khadijeh Karbalaie ◽  
Abbas Kiani ◽  
Mohammad Mardani ◽  
...  
Keyword(s):  
Schwann Cells ◽  
Adult Rat ◽  
Novel Method ◽  
Enriched Cultures

Primary epithelial cell culture of adult rat kidney, enhancement of cell growth by ammonium acetate

1979 ◽  
Vol 121 (1) ◽  
pp. 47-54 ◽  
Author(s):  
Jules Berman ◽  
Alan Perantoni ◽  
Hester Marie Jackson ◽  
Elizabeth Kingsbury
Keyword(s):  
Cell Culture ◽  
Cell Growth ◽  
Epithelial Cell ◽  
Ammonium Acetate ◽  
Rat Kidney ◽  
Epithelial Cell Culture ◽  
Adult Rat ◽  
Primary Epithelial Cell

scholarly journals Neuromuscular Development and Disease: Learning From in vitro and in vivo Models

2021 ◽  
Vol 9 ◽  
Author(s):  
Zachary Fralish ◽  
Ethan M. Lotz ◽  
Taylor Chavez ◽  
Alastair Khodabukus ◽  
Nenad Bursac
Keyword(s):  
Skeletal Muscle ◽  
Cell Culture ◽  
Animal Models ◽  
Schwann Cells ◽  
Motor Neurons ◽  
Small Animal ◽  
In Vivo Models ◽  
Small Animal Models

The neuromuscular junction (NMJ) is a specialized cholinergic synaptic interface between a motor neuron and a skeletal muscle fiber that translates presynaptic electrical impulses into motor function. NMJ formation and maintenance require tightly regulated signaling and cellular communication among motor neurons, myogenic cells, and Schwann cells. Neuromuscular diseases (NMDs) can result in loss of NMJ function and motor input leading to paralysis or even death. Although small animal models have been instrumental in advancing our understanding of the NMJ structure and function, the complexities of studying this multi-tissue system in vivo and poor clinical outcomes of candidate therapies developed in small animal models has driven the need for in vitro models of functional human NMJ to complement animal studies. In this review, we discuss prevailing models of NMDs and highlight the current progress and ongoing challenges in developing human iPSC-derived (hiPSC) 3D cell culture models of functional NMJs. We first review in vivo development of motor neurons, skeletal muscle, Schwann cells, and the NMJ alongside current methods for directing the differentiation of relevant cell types from hiPSCs. We further compare the efficacy of modeling NMDs in animals and human cell culture systems in the context of five NMDs: amyotrophic lateral sclerosis, myasthenia gravis, Duchenne muscular dystrophy, myotonic dystrophy, and Pompe disease. Finally, we discuss further work necessary for hiPSC-derived NMJ models to function as effective personalized NMD platforms.

CD44 is expressed in non-myelinating Schwann cells of the adult rat, and may play a role in neurodegeneration-induced glial plasticity at the neuromuscular junction

2009 ◽  
Vol 34 (2) ◽  
pp. 245-258 ◽  
Author(s):  
Adam Gorlewicz ◽  
Jakub Wlodarczyk ◽  
Ewa Wilczek ◽  
Maciej Gawlak ◽  
Anna Cabaj ◽  
...  
Keyword(s):  
Neuromuscular Junction ◽  
Schwann Cells ◽  
Adult Rat

Comparative study on the replication of HCV1b genome between wild‐type and cell culture‐adaptive mutant in regard to sensitivities against anti‐HCV drugs

2020 ◽  
Vol 64 (4) ◽  
pp. 296-303
Author(s):  
Yohei Miyayama ◽  
Heini Lee ◽  
HoJoong Song ◽  
Hiromi Abe‐Chayama ◽  
Daiki Miki ◽  
...  
Keyword(s):  
Cell Culture ◽  
Comparative Study ◽  
Wild Type

scholarly journals A simple electrical stimulation cell culture system on the myelination of dorsal root ganglia and Schwann cells

BioTechniques ◽  
2019 ◽  
Vol 67 (1) ◽  
pp. 11-15
Author(s):  
Zhuowen Liang ◽  
Tao Lei ◽  
Shuang Wang ◽  
ZhuoJing Luo ◽  
XueYu Hu
Keyword(s):  
Cell Culture ◽  
Electrical Stimulation ◽  
Schwann Cells ◽  
Dorsal Root Ganglia ◽  
Culture System ◽  
Dorsal Root ◽  
Cell Culture System
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