Gene expression profiling of ciliary neurotrophic factor-overexpressing rat striatal progenitor cells (ST14A) indicates improved stress response during the early stage of differentiation

2003 ◽  
Vol 73 (1) ◽  
pp. 42-53 ◽  
Author(s):  
Tobias Böttcher ◽  
Eilhard Mix ◽  
Dirk Koczan ◽  
Peter Bauer ◽  
Jens Pahnke ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stress Response ◽  
Gene Expression Profiling ◽  
Progenitor Cells ◽  
Neurotrophic Factor ◽  
Expression Profiling ◽  
Early Stage ◽  
Ciliary Neurotrophic Factor

scholarly journals Gene expression profiling in peripheral blood leukocytes as a new approach for assessment of human stress response

2005 ◽  
Vol 52 (3,4) ◽  
pp. 137-144 ◽  
Author(s):  
Kazuhito Rokutan ◽  
Kyoko Morita ◽  
Kiyoshi Masuda ◽  
Kumiko Tominaga ◽  
Michiyo Shikishima ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stress Response ◽  
Gene Expression Profiling ◽  
Peripheral Blood ◽  
Expression Profiling ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
New Approach ◽  
Human Stress

Abstract 2357: Gene expression profiling robustly predicts the outcome of patients diagnosed with early stage lung adenocarcinoma

2014 ◽  
Author(s):  
Yann Gaston-Mathé ◽  
CHARLES FERTE ◽  
benoit gauthier ◽  
mathilde bateson ◽  
david planchard ◽  
...  
Keyword(s):  
Gene Expression ◽  
Lung Adenocarcinoma ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Early Stage

Global gene expression profiling in early-stage polycystic kidney disease in the Han:SPRD Cy rat identifies a role for RXR signaling

2011 ◽  
Vol 300 (1) ◽  
pp. F177-F188 ◽  
Author(s):  
Masanori Kugita ◽  
Kazuhiro Nishii ◽  
Miwa Morita ◽  
Daisuke Yoshihara ◽  
Hiroe Kowa-Sugiyama ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Proliferation ◽  
Kidney Disease ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Early Stage ◽  
Global Gene Expression ◽  
Rt Pcr ◽  
Polycystic Kidney ◽  
Global Gene Expression Profiling

Han:SPRD Cy is a spontaneous rat model of polycystic kidney disease (PKD) caused by a missense mutation in Pkdr1. Cystogenesis in this model is not clearly understood. In the current study, we performed global gene expression profiling in early-stage PKD cyst development in Cy/Cy kidneys and normal (+/+) kidneys at 3 and 7 days of postnatal age. Expression profiles were determined by microarray analysis, followed by validation with real-time RT-PCR. Genes were selected with over 1.5-fold expression changes compared with age-matched +/+ kidneys for canonical pathway analysis. We found nine pathways in common between 3- and 7-day Cy/Cy kidneys. Three significantly changed pathways were designated “Vitamin D Receptor (VDR)/Retinoid X Receptor (RXR) Activation,” “LPS/IL-1-Mediated Inhibition of RXR Function,” and “Liver X Receptor (LXR)/RXR Activation.” These results suggest that RXR-mediated signaling is significantly altered in developing kidneys with mutated Pkdr1. In gene ontology analysis, the functions of these RXR-related genes were found to be involved in regulating cell proliferation and organ morphogenesis. With real-time RT-PCR analysis, the upregulation of Ptx2, Alox15b, OSP, and PCNA, major markers of cell proliferation associated with the RXR pathway, were confirmed in 3- and 7-day Cy/Cy kidneys compared with 3-day +/+ kidneys. The increased RXR protein was observed in both the nucleus and cytoplasm of cystic epithelial cells in early-stage Cy/Cy kidneys, and the RXR-positive cells were strongly positive for PCNA staining. Taken together, cell proliferation and organ morphogenesis signals transduced by RXR-mediated pathways may have important roles for cystogenesis in early-stage PKD in this Pkdr1-mutated Cy rat.

scholarly journals Roles for Sphingolipid Biosynthesis in Mediation of Specific Programs of the Heat Stress Response Determined through Gene Expression Profiling

2003 ◽  
Vol 278 (32) ◽  
pp. 30328-30338 ◽  
Author(s):  
L. Ashley Cowart ◽  
Yasuo Okamoto ◽  
Francisco R. Pinto ◽  
Jason L. Gandy ◽  
Jonas S. Almeida ◽  
...  
Keyword(s):  
Gene Expression ◽  
Heat Stress ◽  
Stress Response ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Heat Stress Response ◽  
Sphingolipid Biosynthesis

Clonotypic Bone Marrow-Derived Endothelial Progenitor Cells in Multiple Myeloma.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 3497-3497
Author(s):  
Marc J. Braunstein ◽  
Daniel R. Carrasco ◽  
David Kahn ◽  
Kumar Sukhdeo ◽  
Alexei Protopopov ◽  
...  
Keyword(s):  
Gene Expression ◽  
Multiple Myeloma ◽  
Bone Marrow ◽  
High Resolution ◽  
Gene Expression Profiling ◽  
Progenitor Cells ◽  
Tumor Cells ◽  
Endothelial Progenitor Cells ◽  
Expression Profiling ◽  
Gains And Losses

Abstract In multiple myeloma (MM), bone marrow-derived endothelial progenitor cells (EPCs) contribute to tumor neoangiogenesis and their levels covary with tumor mass and prognosis. Recent X-chromosome inactivation studies in female patients showed that, similar to tumor cells, EPCs are clonally restricted in MM. Genomic profiling of MM using high-resolution array comparative genomic hybridization (aCGH) has been previously utilized to mine the genome and find clinical correlates in MM patients. In this study, clonotypic aspects of bone marrow-derived EPCs and MM cells were investigated using aCGH and expression profiling analysis. Confluent EPCs were outgrown from bone marrow aspirates by adherence to laminin. EPCs were >98% vWF/CD133/KDR+ and <1% CD38+. The laminin-nonadherent bone marrow fraction enriched for tumor cells was >50% CD38+. For aCGH and for gene expression profiling, genomic DNA and total RNA from EPCs and MM cells were hybridized to human oligonucleotide arrays (Agilent Technologies) and human cDNA microarrays (Affymetrix), respectively. High resolution aCGH with segmentation analysis showed that EPCs and MM cells in one of ten cases share identical patterns of chromosomal gains and losses, while another 5 cases shared multiple focal copy number alterations (CNAs) including gains and losses. The genomes of EPCs and MM cells additionally displayed exclusive CNAs, but these were far fewer in EPCs than in MM cells. In 3 patients, EPCs harbored a common 0.6Mb deletion at 1q21 not shared by MM cells. Pertinent genes in this region that could affect proliferation and tumor suppression include N2N, NBPF10, and TXNIP. Validation studies of aCGH findings by other methods are ongoing. Gene expression profiling showed decreased expression of 1q21 region genes (e.g., calgranulin C and lamin A/C). A genome-wide comparison of patients’ MM cells and EPCs, which is focused on their shared genetic characteristics, will be presented.

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