Comparison of Seven Heterophile Antibody Assays for Laboratory Diagnosis of Infectious Mononucleosis in Pediatric Patients

2021 ◽  
Author(s):  
Sharon Wei ◽  
Wei Huang ◽  
Mohamed Farah ◽  
Aaruni Khanolkar ◽  
Xiaotian Zheng
Keyword(s):  
Infectious Mononucleosis ◽  
Pediatric Patients ◽  
Laboratory Diagnosis ◽  
Heterophile Antibody ◽  
Antibody Assays

Acquired Circulating Anticoagulants in Children With Acquired Immunodeficiency Syndrome

PEDIATRICS ◽  
1988 ◽  
Vol 82 (5) ◽  
pp. 763-765
Author(s):  
Edward R. Burns ◽  
Ben-Zion Krieger ◽  
Larry Bernstein ◽  
Arye Rubinstein
Keyword(s):  
Acquired Immunodeficiency Syndrome ◽  
Pediatric Patients ◽  
Opportunistic Infections ◽  
Laboratory Diagnosis ◽  
Activated Partial Thromboplastin Time ◽  
Test Results ◽  
Coagulation Test ◽  
Intrinsic Pathway ◽  
Acquired Immunodeficiency ◽  
Immunodeficiency Syndrome

The mechanism underlying the prolonged activated partial thromboplastin time (APTT) seen in some pediatric patients with acquired immunodeficiency syndrome (AIDS) and opportunistic infections was studied. A circulating inhibitor of coagulation was demonstrated in three patients. The inhibitor appears to be an immunoglobulin that interferes with some of the phospolipid-dependent coagulation reactions of the intrinsic pathway. This "AIDS anticoagulant" does not predispose the patient to clinical bleeding despite its ability to cause a marked prolongation of the APTT. As such, careful laboratory diagnosis of the cause of abnormal coagulation test results is necessary for children with AIDS.

scholarly journals Erratam: Epstein-Barr virus BZLF1 gene promoter variants in pediatric patients with acute infectious mononucleosis. Its comparison with pediatric lymphomas

2012 ◽  
Vol 84 (10) ◽  
pp. 1697-1697
Author(s):  
Mario Alejandro Lorenzetti ◽  
Marina Inés Gutiérrez ◽  
Jaime Altcheh ◽  
Guillermo Moscatelli ◽  
Samanta Moroni ◽  
...  
Keyword(s):  
Infectious Mononucleosis ◽  
Epstein Barr Virus ◽  
Pediatric Patients ◽  
Gene Promoter ◽  
Barr Virus ◽  
Bzlf1 Gene ◽  
Epstein Barr ◽  
Acute Infectious Mononucleosis

Hypercortisolism in Childhood: Shortcomings of Conventional Diagnostic Criteria

PEDIATRICS ◽  
1975 ◽  
Vol 56 (5) ◽  
pp. 797-803
Author(s):  
D. H.P. Streeten ◽  
F. H. Faas ◽  
M. J. Elders ◽  
T. G. Dalakos ◽  
M. Voorhess
Keyword(s):  
Linear Growth ◽  
Pediatric Patients ◽  
Laboratory Diagnosis ◽  
Cortisol Secretion ◽  
Normal Range ◽  
Plasma Growth Hormone ◽  
Urinary Creatinine ◽  
Adrenocortical Hyperplasia ◽  
Suppression Test ◽  
The One

Two patients are described in whom hypercortisolism occurred prepubertally as a consequence of bilateral adrenocortical hyperplasia. In contrast with the manifestalions of Cushing's syndrome in adults, these children presented with obesity and reduced stature and no other symptoms. Both patients excreted amounts of urinary 17OHCS before and during a conventional suppression test with dexamethasone (0.5 mg every six hours) which were within the usual normal range. However, when urinary 17OHCS excretion was expressed per gram of urinary creatinine or per square meter of surface area, and when the dose of dexamethasone was tailored to body mass (2Oµg/kg/day) the results were clearly abnormal, as were plasma corticoids and (in one patient) cortisol secretion rate. Resumption of linear growth occurred after bilateral adrenalectomy in both patients and was associated, in the one patient so studied, by a return of hypoglycemia-stimulated increases in plasma growth hormone levels from previously suppressed values to the normal range, and by a slight increase in the fasting plasma somatomedin concentration. The observations suggest that pediatric patients with hypercortisolism are likely to be overlooked when conventional criteria for laboratory diagnosis are used, but can be recognized by the simple diagnostic modifications used in these studies.

scholarly journals Impact of antigenic diversity on laboratory diagnosis of Avian bornavirus infections in birds

2014 ◽  
Vol 26 (6) ◽  
pp. 769-777 ◽  
Author(s):  
Vanessa Zimmermann ◽  
Monika Rinder ◽  
Bernd Kaspers ◽  
Peter Staeheli ◽  
Dennis Rubbenstroth
Keyword(s):  
Matrix Protein ◽  
Fluorescent Antibody ◽  
Laboratory Diagnosis ◽  
Cross Reactivity ◽  
Diagnostic Tools ◽  
Phylogenetic Groups ◽  
Antigenic Diversity ◽  
Antibody Assays ◽  
Causative Agents ◽  
Antibody Titers

Avian bornaviruses (ABVs) are a group of genetically diverse viruses within the Bornaviridae family that can infect numerous avian species and represent the causative agents of proventricular dilatation disease, an often fatal disease that is widely distributed in captive populations of parrots and related species. The current study was designed to assess the antigenic variability of the family Bornaviridae and to determine its impact on ABV diagnosis by employing fluorescent antibody assays. It was shown that polyclonal rabbit sera directed against recombinant bornavirus nucleoprotein, X protein, phosphoprotein, and matrix protein provided sufficient cross-reactivity for the detection of viral antigen from a broad range of bornavirus genotypes grown in cell culture. In contrast, a rabbit anti-glycoprotein serum and 2 monoclonal antibodies directed against nucleoprotein and phosphoprotein proteins reacted more specifically. Antibodies were readily detected in sera from avian patients infected with known ABV genotypes if cells persistently infected with a variety of different bornavirus genotypes were used for analysis. For all sera, calculated antibody titers were highest when the homologous or a closely related target virus was used for the assay. Cross-reactivity with more distantly related genotypes of other phylogenetic groups was usually reduced, resulting in titer reduction of up to 3 log units. The presented results contribute to a better understanding of the antigenic diversity of family Bornaviridae and further emphasize the importance of choosing appropriate diagnostic tools for sensitive detection of ABV infections.

scholarly journals Diagnosis of Pneumocystis jirovecii Pneumonia in Pediatric Patients in Serbia, Greece, and Romania. Current Status and Challenges for Collaboration

2020 ◽  
Vol 6 (2) ◽  
pp. 49
Author(s):  
Valentina Arsić Arsenijevic ◽  
Timoleon-Achilleas Vyzantiadis ◽  
Mihai Mares ◽  
Suzana Otasevic ◽  
Athanasios Tragiannidis ◽  
...  
Keyword(s):  
Molecular Diagnosis ◽  
Pediatric Patients ◽  
Fungal Infections ◽  
Laboratory Diagnosis ◽  
Pneumocystis Jirovecii ◽  
Current Status ◽  
Pneumocystis Jirovecii Pneumonia ◽  
Clinical Practices ◽  
Online Questionnaire ◽  
Regional Collaboration

Pneumocystis jirovecii can cause fatal Pneumocystis pneumonia (PcP). Many children have been exposed to the fungus and are colonized in early age, while some individuals at high risk for fungal infections may develop PcP, a disease that is difficult to diagnose. Insufficient laboratory availability, lack of knowledge, and local epidemiology gaps make the problem more serious. Traditionally, the diagnosis is based on microscopic visualization of Pneumocystis in respiratory specimens. The molecular diagnosis is important but not widely used. The aim of this study was to collect initial indicative data from Serbia, Greece, and Romania concerning pediatric patients with suspected PcP in order to: find the key underlying diseases, determine current clinical and laboratory practices, and try to propose an integrative future molecular perspective based on regional collaboration. Data were collected by the search of literature and the use of an online questionnaire, filled by relevant scientists specialized in the field. All three countries presented similar clinical practices in terms of PcP prophylaxis and clinical suspicion. In Serbia and Greece the hematology/oncology diseases are the main risks, while in Romania HIV infection is an additional risk. Molecular diagnosis is available only in Greece. PcP seems to be under-diagnosed and regional collaboration in the field of laboratory diagnosis with an emphasis on molecular approaches may help to cover the gaps and improve the practices.

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