scholarly journals Comparison of Nasopharyngeal Swab and Nasopharyngeal Aspiration in Adults for SARS‐CoV‐2 Identification Using Reverse Transcription Polymerase Chain Reaction

2021 ◽  
Author(s):  
Selin ÜNSALER ◽  
Ayşe OKAN ◽  
Süda TEKİN ◽  
Ayşenur MERIÇ HAFIZ ◽  
Ozan GÖKLER ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Nasopharyngeal Swab ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2

2021 ◽  
Vol 12 ◽  
Author(s):  
Jadranka Milosevic ◽  
Mengrou Lu ◽  
Wallace Greene ◽  
Hong-Zhang He ◽  
Si-Yang Zheng
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Polymerase Chain Reaction Assay ◽  
Qpcr Assay ◽  
Nasopharyngeal Swab ◽  
Chain Reaction ◽  
Running Time ◽  
Clinical Sensitivity ◽  
One Step ◽  
Polymerase Chain

We developed an ultrafast one-step RT-qPCR assay for SARS-CoV-2 detection, which can be completed in only 30 min on benchtop Bio-Rad CFX96. The assay significantly reduces the running time of conventional RT-qPCR: reduced RT step from 10 to 1 min, and reduced the PCR cycle of denaturation from 10 to 1 s and extension from 30 to 1 s. A cohort of 60 nasopharyngeal swab samples testing showed that the assay had a clinical sensitivity of 100% and a clinical specificity of 100%.

scholarly journals Occurrence and Timing of Subsequent Severe Acute Respiratory Syndrome Coronavirus 2 Reverse-transcription Polymerase Chain Reaction Positivity Among Initially Negative Patients

2020 ◽  
Author(s):  
Dustin R Long ◽  
Saurabh Gombar ◽  
Catherine A Hogan ◽  
Alexander L Greninger ◽  
Vikas O’Reilly-Shah ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Severe Acute Respiratory Syndrome ◽  
Reverse Transcription ◽  
Polymerase Chain Reaction Test ◽  
Nasopharyngeal Swab ◽  
Chain Reaction ◽  
Reaction Test ◽  
Polymerase Chain ◽  
Using Data ◽  
Chain Reaction Test

Abstract Using data for 20 912 patients from 2 large academic health systems, we analyzed the frequency of severe acute respiratory syndrome coronavirus 2 reverse-transcription polymerase chain reaction test discordance among individuals initially testing negative by nasopharyngeal swab who were retested on clinical grounds within 7 days. The frequency of subsequent positivity within this window was 3.5% and was similar across institutions.

1504: Molecular Diagnosis and Staging of Prostate Cancer Using Clusterin in Real Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)

2006 ◽  
Vol 175 (4S) ◽  
pp. 485-486
Author(s):  
Sabarinath B. Nair ◽  
Christodoulos Pipinikas ◽  
Roger Kirby ◽  
Nick Carter ◽  
Christiane Fenske
Keyword(s):  
Prostate Cancer ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Molecular Diagnosis ◽  
Reverse Transcription ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain
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