scholarly journals Evaluation of a chemiluminescent enzyme immunoassay‐based high‐throughput SARS‐CoV‐2 antigen assay for the diagnosis of COVID‐19: The VITROS ® SARS‐CoV‐2 Antigen Test

2021 ◽  
Author(s):  
Nanako Matsuzaki ◽  
Yuta Orihara ◽  
Masahiro Kodana ◽  
Yutaro Kitagawa ◽  
Masaru Matsuoka ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
High Throughput ◽  
Antigen Test ◽  
Antigen Assay ◽  
Chemiluminescent Enzyme Immunoassay

scholarly journals Evaluation of A Chemiluminescent Enzyme Immunoassay-Based High-Throughput SARS-CoV-2 Antigen Assay For The Diagnosis of COVID-19: The VITROS® SARS-CoV-2 Antigen Test

2021 ◽  
Author(s):  
Nanako Matsuzaki ◽  
Yuta Orihara ◽  
Masahiro Kodana ◽  
Yutaro Kitagawa ◽  
Masaru Matsuoka ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
High Throughput ◽  
Nasopharyngeal Swab ◽  
Antigen Test ◽  
Chain Reaction ◽  
Viral Loads ◽  
Antigen Assay ◽  
Polymerase Chain ◽  
Dose Dependent ◽  
Chemiluminescent Enzyme Immunoassay

Abstract A high-throughput, fully automated antigen detection test for SARS-CoV-2 is a viable alternative to reverse transcription polymerase chain reaction (RT-qPCR) for mass screening during outbreaks. In this study, we compared RT-qPCR for viral load and the VITROS® SARS-CoV-2 Antigen Test with reference to the results of the LUMIPULSE® SARS-CoV-2 Ag Test. Of 128 nasopharyngeal swab specimens taken from patients suspected of being infected with SARS-CoV-2, 49 were positive and 79 were negative according to RT-qPCR. Consistent dose-dependent detection with VITROS® assay was successfully achieved when using nasopharyngeal swab specimens with Ct values of ≤32.0, whereas the CLEIA-based the LUMIPULSE® assay was able to detect lower viral loads compared with the VITROS® assay. Our results show that the performance of the VITROS® assay was satisfactory for the diagnosis of contagious COVID-19 patients in the clinical setting.

High-throughput polymerase chain reaction chemiluminescent enzyme immunoassay for typing and quantifying human papillomavirus DNAs

2004 ◽  
Vol 332 (2) ◽  
pp. 349-357 ◽  
Author(s):  
Simone Ambretti ◽  
Mara Mirasoli ◽  
Simona Venturoli ◽  
Marialuisa Zerbini ◽  
Mario Baraldini ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Human Papillomavirus ◽  
Enzyme Immunoassay ◽  
High Throughput ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Chemiluminescent Enzyme Immunoassay

scholarly journals Immunochromatography and chemiluminescent enzyme immunoassay for COVID-19 diagnosis

2021 ◽  
Vol 27 (6) ◽  
pp. 915-918 ◽  
Author(s):  
Toshiaki Ishii ◽  
Masakazu Sasaki ◽  
Kageto Yamada ◽  
Daiki Kato ◽  
Hiroyoshi Osuka ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Chemiluminescent Enzyme Immunoassay

Development and Validation of an Ultrasensitive Chemiluminescent Enzyme Immunoassay for Aflatoxin M1in Milk

2005 ◽  
Vol 53 (9) ◽  
pp. 3300-3305 ◽  
Author(s):  
Maria Magliulo ◽  
Mara Mirasoli ◽  
Patrizia Simoni ◽  
Rosella Lelli ◽  
Ottavio Portanti ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Development And Validation ◽  
Chemiluminescent Enzyme Immunoassay

Evaluation of cardiac assays on a benchtop chemiluminescent enzyme immunoassay analyzer, PATHFAST

2008 ◽  
Vol 375 (1) ◽  
pp. 144-146 ◽  
Author(s):  
Takashi Kurihara ◽  
Atsushi Yanagida ◽  
Hiroyuki Yokoi ◽  
Atsushi Koyata ◽  
Takeshi Matsuya ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Chemiluminescent Enzyme Immunoassay

scholarly journals CORRELATION OF MONOCYTE COUNT, MLR AND NLCR WITH PRESEPSIN LEVEL IN SIRS (Hubungan Jumlah Monosit, MLR dan NLCR dengan Kadar Presepsin pada SIRS)

2018 ◽  
Vol 22 (3) ◽  
pp. 212
Author(s):  
Nurmalia PS ◽  
N. Suci W ◽  
Imam BW
Keyword(s):  
Systemic Inflammatory Response Syndrome ◽  
Inflammatory Response ◽  
Enzyme Immunoassay ◽  
Lymphocyte Count ◽  
Systemic Inflammatory Response ◽  
Monocyte Count ◽  
Lymphocyte Ratio ◽  
Count Ratio ◽  
Hematology Analyzer ◽  
Chemiluminescent Enzyme Immunoassay

Systemic Inflammatory Response Syndrome (SIRS) mempunyai kebahayaan tinggi terjadi sepsis dan kematian. Nilai jumlahkeseluruhan leukosit merupakan salah satu peramal pasien SIRS dengan bakteriemia. Pemeriksaan jumlah monosit, angka bandinglimfosit Monocyte-Lymphocyte Ratio (MLR), Neutrophil-Lymphocyte Count Ratio (NLCR) dapat diketahui dengan pemeriksaan leukosit.Presepsin telah diteliti untuk mencerminkan kondisi sepsis. Penelitian ini bertujuan untuk mengetahui keberadaan hubungan jumlahmonosit, MLR dan NLCR dengan presepsin di SIRS lewat pembuktian. Ada 34 pasien SIRS di ICU RSUP Dr. Kariadi, diambil secaraberturutan antara selama bulan Januari−Februari 2014. Pemeriksaan darah rutin dengan hematology analyzer. MLR dan NLCR dihitung secara manual. Kadar presepsin ditentukan dengan metode Chemiluminescent Enzyme Immunoassay (CLEIA). Uji kenasabanPearson untuk hubungan MLR dan NLCR dengan presepsin. Uji kenasaban Spearman untuk jumlah monosit dengan presepsin. Kadarpresepsin subjek penelitian 286–15687 pg/mL. Terdapat 23(67,8%) subjek yang mempunyai jumlah monosit dalam rentang nilai rujukan.24(70,6%) dan memiliki jumlah neutrofil absolut lebih besar dari rentang nilai rujukan, sedangkan 21(61,8%) mempunyai jumlahlimfosit absolut dalam rentang nilai rujukan. Hubungan jumlah monosit dengan presepsin mempunyai nilai r= -0,204; p=0,247;yang terkait MLR dengan presepsin r=0,163; p=0,358; sedangkan NLCR dengan presepsin r=0,345; p=0,046. Didasari telitian ini,dapat disimpulkan tidak terdapat hubungan bermakna antara jumlah monosit dan MLR dengan presepsin, selain itu didapatkan pulahubungan positif berarti antara NLCR dan presepsin di SIRS.

Sign in / Sign up

Export Citation Format

Share Document