High‐risk HPV genotypes in cervical neoplasias in Senegal; Respective prevalence of high‐risk HPVgenotypes in cervical neoplasia in Senegal

2021 ◽  
Author(s):  
Halimatou Diop‐Ndiaye ◽  
Xavier Sastre‐Garau ◽  
Aboubacry Drame ◽  
Birama Dembele ◽  
Nafissatou N. Ba ◽  
...  
Keyword(s):  
High Risk ◽  
Cervical Neoplasia ◽  
Hpv Genotypes ◽  
High Risk Hpv

Human papillomavirus genotyping using HPV DNA chip analysis in Korean women

2007 ◽  
Vol 17 (2) ◽  
pp. 497-501 ◽  
Author(s):  
H. S. Lee ◽  
K. M. Kim ◽  
S. M. Kim ◽  
Y. D. Choi ◽  
J. H. Nam ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Cervical Neoplasia ◽  
Dna Chip ◽  
Korean Women ◽  
Hpv 16 ◽  
Hpv Dna ◽  
Hpv Genotypes ◽  
High Risk Hpv ◽  
Chip Analysis

This study was designed to investigate the genotypes of human papillomavirus (HPV) in Korean women who had abnormal cervical cytology and to evaluate the clinical accuracy of HPV DNA chip analysis for the diagnosis of cervical neoplasia. Liquid-based cytology preparations, HPV DNA chip analysis, and cervical biopsy were performed in 2358 women. High-risk HPV was identified in 23.5% of 1650 histologically confirmed normal samples (including cervicitis and squamous metaplasia) and in 81.8% of 708 samples with cervical intraepithelial neoplasia (CIN) and carcinoma (P< 0.01). The major prevalent high-risk HPV genotypes in 381 samples of CIN II/III were HPV-16, -58, -33, and -31, in order of prevalence rate (average overall, 78.0%), and HPV-16, -18, -58, and -33 (average overall, 81.2%) in 133 samples of squamous cell carcinoma (SCC). The infection rate of HPV-16 was significantly higher than that of other high-risk HPV genotypes in all normal, CIN, and SCC cases (P< 0.01) and increased with more advanced squamous cervical lesions (P< 0.01). The detection accuracy of high-risk HPV using HPV DNA chip analysis for CIN II or worse was as follows: sensitivity 84% (81–87%), specificity 72% (70–74%), positive predictive value 47% (44–50%), and negative predictive value 94% (92–95%). These results suggest that HPV DNA chip analysis may be a reliable diagnostic tool for the detection of cervical neoplasia and that there are geographic differences in the distribution of high-risk HPV genotypes.

Characteristics of HSV-2, M.genitalium and C.trachomatis in HPV genotypes- associated Cervical Cancer and Genital Infections...

2019 ◽  
Vol 20 ◽  
Author(s):  
Mona Moharreri ◽  
Amir Sohrabi
Keyword(s):  
Cervical Cancer ◽  
High Risk ◽  
Descriptive Study ◽  
Cervical Neoplasia ◽  
Genital Infections ◽  
Common Cancer ◽  
Hpv Genotypes ◽  
Hpv Types ◽  
Liquid Based Cytology ◽  
High Risk Hpv

Background: Cervical cancer is the most common cancer in women. High Risk HPV types are known as the main agents involved in genital and cervical malignancies. There may be co pathogens like STDs that involved in enhancing the susceptibility and progression to cervical neoplasia. This study was conducted to detect HSV2, C.Trachomatis and M.Genitalium in women with and at the risk of cervical cancer related to HPV for defining their roles in facilitating and persistence of genital disorders. Materials and Methods: This descriptive study was performed on 195 Liquid Based Cytology (LBCs) specimens were collected from women referred to private laboratories. Fifty, 98 and 47 samples were from women with known cervical cancer, HPV positive and HPV negative, respectively. The HSV-2, C.trachomatis, M.genitalium and HPV genotypes have been detected using multiplex TaqMan Real Time PCR and PCR hybridization. Results: A total of 148 HPV positive samples, HPVs 6 (35.13%), 16 (32.43%), 18 (21.62%), 11 (9.46%), 31 (9.46%), and 51 (9.46%) were the most common genotypes. Single, 2, 3, and more than 4 multiple HPV genotypes were detected in 46%, 29.7%, 14.2%, 15 10.1% cases, respectively. The prevalence of M.genitalium, C.trachomatis and HSV2 was 3 (1.54%), 24 (12.3%) and 1(0.5%), respectively. There were no statistically significant differences between these pathogens and cervical cancer (p≥ 0.05). Conclusions: HR-HPV genotypes were more prevalent in genital infections and cervical cancer.

scholarly journals Molecular characterization of high-risk human papillomavirus (HR-HPV) in women in Lomé, Togo

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Akouélé P. Kuassi-Kpede ◽  
Essolakina Dolou ◽  
Théodora M. Zohoncon ◽  
Ina Marie Angèle Traore ◽  
Gnatoulma Katawa ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
West Africa ◽  
Public Health Problem ◽  
Hpv Infection ◽  
Cervical Lesions ◽  
Hpv Genotypes ◽  
Precancerous Cervical Lesions ◽  
High Risk Hpv ◽  
First Time

Abstract Background The causative agent of cervical cancer referred to as Human papillomavirus (HPV) remains a real public health problem. Many countries in West Africa, such as Togo have no data on the high-risk HPV (HR-HPV) infection and genotypes distribution. In order to fill the knowledge gap in the field in Togo, the main objective of this study was to determine the prevalence of pre-cancerous lesions of the cervix and HR-HPV genotypes among Togolese women. Methods Samples were collected from 240 women by introducing a swab in the cervix. Then, the screening of precancerous cervical lesions using the visual inspection with acetic acid and lugol (VIA / VIL) was conducted. The HR-HPV genotypes were characterised by real-time multiplex PCR. Results Out of 240 women recruited, 128 (53.3%) were infected by HR-HPV. The most common genotypes were HPV 56 (22.7%), followed by HPV 51 (20.3%), HPV 31 (19.5%), HPV 52 (18.8%) and HPV 35 (17.2%). The least common genotypes were HPV 33 (2.3%) and HPV 16 (2.3%). Among the women, 1.3% (3/240) were positive to VIA/VIL. Conclusion This study allowed HR-HPV genotypes to be characterised for the first time in Lomé, Togo. This will help in mapping the HR-HPV genotypes in West Africa.

Comparison of Human Papillomavirus Genotypes in Penile Intraepithelial Neoplasia and Associated Lesions: LCM-PCR Study of 87 Lesions in 8 Patients

2019 ◽  
Vol 28 (3) ◽  
pp. 265-272 ◽  
Author(s):  
María José Fernández-Nestosa ◽  
Nuria Guimerà ◽  
Diego F. Sanchez ◽  
Sofía Cañete-Portillo ◽  
Antonella Lobatti ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Precancerous Lesions ◽  
Intraepithelial Neoplasia ◽  
Associated Lesions ◽  
Hpv Genotypes ◽  
Flat Lesions ◽  
High Risk Hpv ◽  
Intraepithelial Lesions ◽  
Penile Intraepithelial Neoplasia

Penile intraepithelial neoplasia (PeIN) is currently classified in human papillomavirus (HPV)- and non-HPV-related subtypes with variable HPV genotypes. PeINs are frequently associated with other intraepithelial lesions in the same specimen. The aim of this study was to detect and compare HPV genotypes in PeINs and associated lesions using high-precision laser capture microdissection-polymerase chain reaction and p16INK4a immunostaining. We evaluated resected penile specimens from 8 patients and identified 33 PeINs and 54 associated lesions. The most common subtype was warty PeIN, followed by warty-basaloid and basaloid PeIN. Associated lesions were classical condylomas (17 cases), atypical classical condylomas (2 cases), flat condylomas (9 cases), atypical flat condylomas (6 cases), flat lesions with mild atypia (12 cases), and squamous hyperplasia (8 cases). After a comparison, identical HPV genotypes were found in PeIN and associated lesions in the majority of the patients (7 of 8 patients). HPV16 was the most common genotype present in both PeIN and corresponding associated lesion (50% of the patients). Nonspecific flat lesions with mild atypia, classical condylomas, and atypical condylomas were the type of associated lesions most commonly related to HPV16. Other high-risk HPV genotypes present in PeIN and associated nonspecific flat lesion with mild atypia were HPV35 and HPV39. In this study of HPV in the microenvironment of penile precancerous lesions, we identified identical high-risk HPV genotypes in PeIN and classical, flat, or atypical condylomas and, specially, in nonspecific flat lesions with mild atypia. It is possible that some of these lesions represent hitherto unrecognized precancerous lesions.

scholarly journals High risk HPV genotypes in Belarus

2014 ◽  
Vol 14 (S2) ◽  
Author(s):  
VF Eremin ◽  
GI Viarheichyk ◽  
EL Gasich ◽  
EA Shishkin ◽  
MV Domnich ◽  
...  
Keyword(s):  
High Risk ◽  
Hpv Genotypes ◽  
High Risk Hpv

High-Risk Human Papillomavirus (HPV) in Parotid Lesions

2007 ◽  
Vol 22 (4) ◽  
pp. 239-244 ◽  
Author(s):  
D. Vageli ◽  
G. Sourvinos ◽  
M. Ioannou ◽  
G.K. Koukoulis ◽  
D.A. Spandidos
Keyword(s):  
Salivary Gland ◽  
Viral Load ◽  
High Risk ◽  
Salivary Gland Tumors ◽  
Specific Primers ◽  
In Situ Pcr ◽  
Hpv Genotypes ◽  
Pleomorphic Adenomas ◽  
High Risk Hpv

Although several studies have reported that oropharyngeal infection with HPV may predispose to tumorigenesis, little is known about the etiological factors of salivary gland tumors and the presence of HPV We studied 9 parotid lesions for HPV infection including an oncocytoma, an acinic cell carcinoma, a high-grade adenocarcinoma, a low-grade polymorphous adenocarcinoma, a Warthin's tumor and 2 pleomorphic adenomas, a lymphoepithelial cyst and a lipoma of the parotid gland. DNA was extracted from formalin-fixed and paraffin-embedded tissue sections. Solution PCR for HPV detection was performed using the GP5+/GP6+ primers, while HPV typing was carried out by multiplex PCR for HPV6, 11, 16, 18, and 33; positive samples were recorfirmed by PCR with specific primers for each type. Quantitative real-time PCR for the high-risk HPV genotypes 16, 18, 31, 33, 35, 52, 58 and 67 was also performed to quantitate the viral load. Finally, in situ PCR was employed with HPV16-specific primers by direct-detection method. Seven of the 9 parotid lesions were HPV positive while 6 of these 7 had been infected by HPV16 and/or HPV18 oncogenic types. High viral load of high-risk genotypes of HPV was found in the oncocytoma, in one of the pleomorphic adenomas, and in the Warthin's tumor. Finally, in situ PCR indicated that HPV16 amplification occurred in the salivary gland tumors. This is the first time that high-risk HPV genotypes are detected in these histological types of parotid lesions, suggesting the possible involvement of the virus in the disease.

scholarly journals p16INK4a Expression Correlates with Degree of Cervical Neoplasia: A Comparison with Ki-67 Expression and Detection of High-Risk HPV Types

2003 ◽  
Vol 16 (7) ◽  
pp. 665-673 ◽  
Author(s):  
S Nicholas Agoff ◽  
Patricia Lin ◽  
Janice Morihara ◽  
Constance Mao ◽  
Nancy B Kiviat ◽  
...  
Keyword(s):  
High Risk ◽  
Cervical Neoplasia ◽  
Ki 67 ◽  
P16ink4a Expression ◽  
Hpv Types ◽  
High Risk Hpv
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