The recombinant Newcastle disease virus Anhinga strain expressing human TRAIL exhibit antitumor effects on a glioma nude mice model

2020 ◽  
Author(s):  
Jinjiao He ◽  
Ying An ◽  
Jianying Qi ◽  
Lin Cui ◽  
Kai Yang ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Nude Mice ◽  
Newcastle Disease ◽  
Mice Model ◽  
Antitumor Effects ◽  
Nude Mice Model ◽  
Recombinant Newcastle Disease Virus

Protection and Differentiation of Infected from Vaccinated Animals by an Inactivated Recombinant Newcastle Disease Virus/Avian Influenza H5 Vaccine

2010 ◽  
Vol 5 (s1) ◽  
pp. e23-e24
Author(s):  
Bernardo Lozano-Dubernard ◽  
Ernesto Soto-Priante ◽  
David Sarfati-Mizrahi ◽  
Felipa Castro-Peralta ◽  
Ricardo Flores-Castro ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Recombinant Newcastle Disease Virus

scholarly journals An improved method for the rescue of recombinant Newcastle disease virus

BioTechniques ◽  
2020 ◽  
Vol 68 (2) ◽  
pp. 96-100
Author(s):  
Pheik-Sheen Cheow ◽  
Tiong Kit Tan ◽  
Adelene Ai-Lian Song ◽  
Khatijah Yusoff ◽  
Suet Lin Chia
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Reverse Genetics ◽  
Vaccine Development ◽  
Transfection Efficiency ◽  
Transfection Method ◽  
Helper Plasmids ◽  
Immunogenic Properties ◽  
Recombinant Newcastle Disease Virus

Reverse genetics has been used to generate recombinant Newcastle disease virus with enhanced immunogenic properties for vaccine development. The system, which involves co-transfecting the viral antigenomic plasmid with three helper plasmids into a T7 RNA polymerase-expressing cell to produce viral progenies, poses a great challenge. We have modified the standard transfection method to improve the transfection efficiency of the plasmids, resulting in a higher titer of virus progeny production. Two transfection reagents (i.e., lipofectamine and polyethylenimine) were used to compare the transfection efficiency of the four plasmids. The virus progenies produced were quantitated with flow cytometry analysis of the infectious virus unit. The modified transfection method increased the titer of virus progenies compared with that of the standard transfection method.

Rescue of a recombinant Newcastle disease virus strain R2B expressing green fluorescent protein

Virus Genes ◽  
2017 ◽  
Vol 53 (3) ◽  
pp. 410-417 ◽  
Author(s):  
Madhan Mohan Chellappa ◽  
Sohini Dey ◽  
Satish Gaikwad ◽  
Dinesh C. Pathak ◽  
Vikram N. Vakharia
Keyword(s):  
Green Fluorescent Protein ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Virus Strain ◽  
Newcastle Disease ◽  
Fluorescent Protein ◽  
Newcastle Disease Virus Strain ◽  
Green Fluorescent ◽  
Recombinant Newcastle Disease Virus
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