Multicenter evaluation of Xpert HIV‐1 viral load assay for HIV quantification in China

2020 ◽  
Vol 92 (12) ◽  
pp. 3295-3300
Author(s):  
Lifeng Liu ◽  
Renni Deng ◽  
Yun Lan ◽  
Li Li ◽  
Mei Li ◽  
...  
Keyword(s):  
Viral Load ◽  
Viral Load Assay ◽  
Hiv 1

scholarly journals Performance of the Abbott RealTime HIV-1 Viral Load Assay Is Not Impacted by Integrase Inhibitor Resistance-Associated Mutations

2011 ◽  
Vol 49 (4) ◽  
pp. 1631-1634 ◽  
Author(s):  
T. P. Young ◽  
G. Cloherty ◽  
S. Fransen ◽  
L. Napolitano ◽  
P. Swanson ◽  
...  
Keyword(s):  
Viral Load ◽  
Integrase Inhibitor ◽  
Viral Load Assay ◽  
Inhibitor Resistance ◽  
Hiv 1

scholarly journals Performance of a high-throughput next-generation sequencing method for analysis of HIV drug resistance and viral load

2020 ◽  
Vol 75 (12) ◽  
pp. 3510-3516 ◽  
Author(s):  
Jessica M Fogel ◽  
David Bonsall ◽  
Vanessa Cummings ◽  
Rory Bowden ◽  
Tanya Golubchik ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Viral Load ◽  
Next Generation Sequencing ◽  
High Throughput ◽  
Whole Genome ◽  
Hiv Viral Load ◽  
Viral Loads ◽  
Viral Load Assay ◽  
Generation Sequencing ◽  
Hiv 1

Abstract Objectives To evaluate the performance of a high-throughput research assay for HIV drug resistance testing based on whole genome next-generation sequencing (NGS) that also quantifies HIV viral load. Methods Plasma samples (n = 145) were obtained from HIV-positive MSM (HPTN 078). Samples were analysed using clinical assays (the ViroSeq HIV-1 Genotyping System and the Abbott RealTime HIV-1 Viral Load assay) and a research assay based on whole-genome NGS (veSEQ-HIV). Results HIV protease and reverse transcriptase sequences (n = 142) and integrase sequences (n = 138) were obtained using ViroSeq. Sequences from all three regions were obtained for 100 (70.4%) of the 142 samples using veSEQ-HIV; results were obtained more frequently for samples with higher viral loads (93.5% for 93 samples with >5000 copies/mL; 50.0% for 26 samples with 1000–5000 copies/mL; 0% for 23 samples with <1000 copies/mL). For samples with results from both methods, drug resistance mutations (DRMs) were detected in 33 samples using ViroSeq and 42 samples using veSEQ-HIV (detection threshold: 5.0%). Overall, 146 major DRMs were detected; 107 were detected by both methods, 37 were detected by veSEQ-HIV only (frequency range: 5.0%–30.6%) and two were detected by ViroSeq only. HIV viral loads estimated by veSEQ-HIV strongly correlated with results from the Abbott RealTime Viral Load assay (R2 = 0.85; n = 142). Conclusions The NGS-based veSEQ-HIV method provided results for most samples with higher viral loads, was accurate for detecting major DRMs, and detected mutations at lower levels compared with a method based on population sequencing. The veSEQ-HIV method also provided HIV viral load data.

A RealTime HIV-1 viral load assay for automated quantitation of HIV-1 RNA in genetically diverse group M subtypes A–H, group O and group N samples

2007 ◽  
Vol 146 (1-2) ◽  
pp. 236-245 ◽  
Author(s):  
Ning Tang ◽  
Shihai Huang ◽  
John Salituro ◽  
Wai-Bing Mak ◽  
Gavin Cloherty ◽  
...  
Keyword(s):  
Viral Load ◽  
Diverse Group ◽  
Viral Load Assay ◽  
Hiv 1

Evaluation of the clinical performance of the Xpert HIV-1 viral load assay

2015 ◽  
Vol 70 ◽  
pp. S102
Author(s):  
B. Ouma ◽  
L. Crocombe ◽  
J. Salha ◽  
A. Sy
Keyword(s):  
Viral Load ◽  
Clinical Performance ◽  
Viral Load Assay ◽  
Hiv 1

scholarly journals Performance characteristics of the Cavidi ExaVir viral load assay and the ultra-sensitive P24 assay relative to the Roche Monitor HIV-1 RNA assay

2010 ◽  
Vol 49 (3) ◽  
pp. 198-204 ◽  
Author(s):  
Paul Stewart ◽  
Ada Cachafeiro ◽  
Sonia Napravnik ◽  
Joseph J. Eron ◽  
Ian Frank ◽  
...  
Keyword(s):  
Viral Load ◽  
Performance Characteristics ◽  
Viral Load Assay ◽  
Hiv 1

Comparison of HIV-1 viral load assay performance in immunological stable patients with low or undetectable viremia

2012 ◽  
Vol 202 (1) ◽  
pp. 67-75 ◽  
Author(s):  
Gudrun Naeth ◽  
Robert Ehret ◽  
Frank Wiesmann ◽  
Patrick Braun ◽  
Heribert Knechten ◽  
...  
Keyword(s):  
Viral Load ◽  
Assay Performance ◽  
Undetectable Viremia ◽  
Viral Load Assay ◽  
Hiv 1

Xpert HIV-1 Viral Load Assay and VERSANT HIV-1 RNA 1.5 Assay

2017 ◽  
Vol 74 (3) ◽  
pp. e86-e88 ◽  
Author(s):  
Bianca Bruzzone ◽  
Patrizia Caligiuri ◽  
Nicola Nigro ◽  
Claudia Arcuri ◽  
Sabrina Delucis ◽  
...  
Keyword(s):  
Viral Load ◽  
Viral Load Assay ◽  
Hiv 1

Multi-site clinical evaluation of the Xpert® HIV-1 viral load assay

2016 ◽  
Vol 80 ◽  
pp. 27-32 ◽  
Author(s):  
J.A. Jordan ◽  
J.C. Plantier ◽  
K. Templeton ◽  
A.H.B. Wu
Keyword(s):  
Viral Load ◽  
Clinical Evaluation ◽  
Viral Load Assay ◽  
Hiv 1
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