Genetic characterization of dengue virus serotype 2 isolated from dengue fever outbreaks in eastern Uttar Pradesh and western Bihar, India

2020 ◽  
Author(s):  
Hirawati Deval ◽  
Sthita P. Behera ◽  
Awdhesh Agrawal ◽  
Rajeev Singh ◽  
Brijranjan Misra ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Dengue Fever ◽  
Genetic Characterization ◽  
Uttar Pradesh ◽  
Virus Serotype ◽  
Eastern Uttar Pradesh ◽  
Dengue Virus Serotype 2

scholarly journals Generation and Characterization of Antinonstructural Protein 1 Monoclonal Antibodies and Development of Diagnostics for Dengue Virus Serotype 2

2017 ◽  
Vol 97 (4) ◽  
pp. 1049-1061 ◽  
Author(s):  
Yin-Liang Tang ◽  
Chun-Yu Lin ◽  
Chien-Yu Chiu ◽  
Han-Chung Wu ◽  
Chwan-Chuen King ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Dengue Virus ◽  
Virus Serotype ◽  
Dengue Virus Serotype 2

scholarly journals Emergence of dengue virus serotype 2 in Mauritania and molecular characterization of its circulation in West Africa

2021 ◽  
Vol 15 (10) ◽  
pp. e0009829
Author(s):  
Toscane Fourié ◽  
Ahmed El Bara ◽  
Audrey Dubot-Pérès ◽  
Gilda Grard ◽  
Sébastien Briolant ◽  
...  
Keyword(s):  
West Africa ◽  
Dengue Virus ◽  
Dengue Fever ◽  
West African ◽  
Capital City ◽  
Epidemic Strain ◽  
African Countries ◽  
Recent Emergence ◽  
Virus Serotype ◽  
Dengue Virus Serotype 2

The number of sporadic and epidemic dengue fever cases have reportedly been increasing in recent years in some West African countries, such as Senegal and Mali. The first epidemic of laboratory-confirmed dengue occurred in Nouakchott, the capital city of Mauritania situated in the Saharan desert, in 2014. On-site diagnosis of dengue fever was established using a rapid diagnostic test for dengue. In parallel, the presence of Aedes aegypti mosquitoes in the city was confirmed. The initial diagnosis was confirmed by RT-PCR, which showed that all samples from the 2014 dengue epidemic in Nouakchott were dengue virus serotype 2 (DENV-2). The whole genome or envelope protein gene of these strains, together with other DENV-2 strains obtained from travelers returning from West African countries to France between 2016 and 2019 (including two Mauritanian strains in 2017 and 2018), were sequenced. Phylogenetic analysis suggested a recent emergence of an epidemic strain from the cosmopolitan genotype belonging to West African cosmopolitan lineage II, which is genetically distinct from African sylvatic genotype. The origin of this DENV-2 lineage is still unknown, but our data seem to suggest a recent and rapid dispersion of the epidemic strain throughout the region. More complete genome sequences of West African DENV-2 are required for a better understanding of the dynamics of its circulation. Arboviral surveillance and outbreak forecasting are urgently needed in West Africa.

scholarly journals Isolation and characterization of dengue virus serotype 2 from the large dengue outbreak in Guangdong, China in 2014

2014 ◽  
Vol 57 (12) ◽  
pp. 1149-1155 ◽  
Author(s):  
Hui Zhao ◽  
LingZhai Zhao ◽  
Tao Jiang ◽  
XiaoFeng Li ◽  
Hang Fan ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Dengue Outbreak ◽  
Isolation And Characterization ◽  
Virus Serotype ◽  
Dengue Virus Serotype 2

scholarly journals Genome Sequence of a Dengue Virus Serotype 2 Strain Identified during the 2019 Outbreak in Bangladesh

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Roly Malaker ◽  
Mohammad S. I. Sajib ◽  
Apurba R. Malaker ◽  
Hafizur Rahman ◽  
Yogesh Hooda ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Dengue Fever ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Virus Serotype ◽  
Dengue Virus Serotype 2

ABSTRACT A nearly complete genome sequence of a dengue virus serotype 2 strain detected in the serum of a patient in 2019 during the largest outbreak of dengue fever in Bangladesh is reported.

scholarly journals Micafungin Inhibits Dengue Virus Infection through the Disruption of Virus Binding, Entry, and Stability

2021 ◽  
Vol 14 (4) ◽  
pp. 338
Author(s):  
Yen-Chen Chen ◽  
Jeng-Wei Lu ◽  
Chia-Tsui Yeh ◽  
Te-Yu Lin ◽  
Feng-Cheng Liu ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Dengue Fever ◽  
Early Stage ◽  
Quantitative Polymerase Chain Reaction ◽  
Enterovirus 71 ◽  
Denv Infection ◽  
Immunofluorescence Assay ◽  
Dependent Manner ◽  
Virus Binding ◽  
Virus Serotype

Dengue fever is an arbovirus disease caused by infection with the dengue virus (DENV). Half of the world’s population lives under the threat of dengue fever, however, researchers have yet to develop any drugs that are clinically applicable to this infection. Micafungin is a member of the echinocandins family of anti-fungal drugs, capable of blocking the synthesis of β-1,3-D-glucan in the walls of fungal cells. Previous studies have demonstrated the effectiveness of Micafungin against infections of enterovirus 71 (EV71) and chikungunya virus (CHIKV). This is the first study demonstrating the effectiveness of micafungin in inhibiting the cytopathic effects of dengue virus serotype 2 (DENV-2) in a dose-dependent manner. Time-of-addition assays verified the inhibitory effects of micafungin in pre-treated, co-treated, and full-treatment groups. Binding and entry assays also demonstrated the effectiveness of micafungin in the early stage of DENV-2 infection. The virucidal efficacy of micafungin appears to lie in its ability to destroy the virion. Molecular docking assays revealed the binding of micafungin to the envelope protein of DENV-2, thereby revealing the mechanism by which micafungin affects the early stage of DENV infection and the stability of DENV. Two other micafungin analogs, caspofungin and anidulafungin, were also shown to have the antiviral effects on DENV-2. Finally, immunofluorescence assay (IFA) and reverse-transcription quantitative polymerase chain reaction (RT-qPCR) confirmed the broad anti-DENV ability of micafungin against dengue virus serotypes 1, 3, and 4 (DENV-1, DENV-3, and DENV-4). Taken together, these results demonstrate the potential of micafungin and its analogs as candidates for the development of broad-spectrum treatments for DENV infection.

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