Induction of peripheral blood T follicular helper cells expressing ICOS correlates with antibody response to hepatitis B vaccination

2019 ◽  
Vol 92 (1) ◽  
pp. 62-70 ◽  
Author(s):  
Mingluan Xing ◽  
Yonghui Feng ◽  
Jun Yao ◽  
Huakun Lv ◽  
Yongdi Chen ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Antibody Response ◽  
Peripheral Blood ◽  
Hepatitis B Vaccination ◽  
T Follicular Helper Cells ◽  
Helper Cells ◽  
Follicular Helper

scholarly journals T follicular helper cells and antibody response to Hepatitis B virus vaccine in HIV-1 infected children receiving ART

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Yonas Bekele ◽  
Desalegn Yibeltal ◽  
Kidist Bobosha ◽  
Temesgen E. Andargie ◽  
Mahlet Lemma ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Antibody Response ◽  
Virus Vaccine ◽  
T Follicular Helper Cells ◽  
Helper Cells ◽  
Follicular Helper ◽  
B Virus ◽  
Hepatitis B Virus Vaccine ◽  
Hiv 1

T Follicular Helper Cells of the Mother Prevent Vertical Transmission of Hepatitis B to their Babies

2016 ◽  
Vol 64 (2) ◽  
pp. S507 ◽  
Author(s):  
A.K. Vyas ◽  
P. David ◽  
S. Patra ◽  
S.K. Sarin ◽  
N. Trehanpati
Keyword(s):  
Hepatitis B ◽  
Vertical Transmission ◽  
T Follicular Helper Cells ◽  
Helper Cells ◽  
Follicular Helper

scholarly journals Loss of T Follicular Helper Cells in the Peripheral Blood of Patients with Chronic Graft-versus-Host Disease

2016 ◽  
Vol 22 (5) ◽  
pp. 825-833 ◽  
Author(s):  
David A. Knorr ◽  
Hongbo Wang ◽  
Mukta Aurora ◽  
Margaret L. MacMillan ◽  
Shernan G. Holtan ◽  
...  
Keyword(s):  
Graft Versus Host Disease ◽  
Peripheral Blood ◽  
T Follicular Helper Cells ◽  
Host Disease ◽  
Graft Versus Host ◽  
Helper Cells ◽  
Follicular Helper

scholarly journals miR-138 Reduces the Dysfunction of T Follicular Helper Cells in Osteosarcoma via the PI3K/Akt/mTOR Pathway by Targeting PDK1

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Baoen Jiang ◽  
Xiuqin Kang ◽  
Gang Zhao ◽  
Jianshu Lu ◽  
Zhitao Wang
Keyword(s):  
Flow Cytometry ◽  
B Cells ◽  
Peripheral Blood ◽  
Mtor Pathway ◽  
Control Group ◽  
T Follicular Helper Cells ◽  
Tfh Cells ◽  
Helper Cells ◽  
Follicular Helper ◽  
Immature B Cells

Objective. To study the effect of miR-138 on the function of osteosarcoma (OS) T follicular helper cells (Tfh cells) and its mechanism. Methods. Peripheral blood mononuclear cells (PBMCs) were isolated from patients with osteosarcoma (OS group) and healthy volunteers (control group). CD4+CXCR5+ Tfh cells and CD9+ B cells were sorted by flow cytometry. qRT-PCR was used to detect the expression of miR-138 and PDK1 in the peripheral blood and CD4+CXCR5+ Tfh cells. Flow cytometry was employed to detect the proportion of CD4+CXCR5+ Tfh cells in CD4+ T cells, the level of CD40L in CD4+CXCR5+ Tfh cells, and the expression of CD27 and CD38 in B cells. Western blot was used to determine the protein expression of PDK1, PI3K, p-Akt, Akt, p-mTOR, and mTOR. In addition, dual-luciferase reporter assay was performed to verify the relationship between miR-138 and PDK1. ELISA method was used to determine the levels of IgM, IgG, IL-10, and IL-21. Results. Compared with that of the control group, the expression of miR-138 in PBMC and CD4+CXCR5+ Tfh cells of the OS group was lower; overexpression of miR-138 could promote the maturation of Tfh cells and immature B cells. The results of the dual-luciferase report experiment showed that miR-138 can target and negatively regulate PDK1, and PDK1 can reverse the effect of miR-138 on the function of Tfh cells and immature B cells. Conclusion. miR-138 inhibits the PI3K/Akt/mTOR pathway by targeting and negatively regulating PDK1 to alleviate the dysfunction of T follicular helper cells in OS.

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