Mutations within the major hydrophilic region (MHR) of Hepatitis B virus from individuals with simultaneous HBsAg and anti-HBs in Guangzhou, Southern China

2018 ◽  
Vol 90 (8) ◽  
pp. 1337-1342 ◽  
Author(s):  
Kemin Liu ◽  
Mingyu Xie ◽  
Xiaomei Lu ◽  
Hui Yu ◽  
Hui Wang ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Southern China ◽  
Major Hydrophilic Region ◽  
B Virus ◽  
Hydrophilic Region

Prevalence of S gene mutations within the major hydrophilic region of hepatitis B virus in patients in Dongguan, southern China

2017 ◽  
Vol 162 (10) ◽  
pp. 2949-2957 ◽  
Author(s):  
Siping Li ◽  
Mingyu Xie ◽  
Wenrui Li ◽  
Qi Peng ◽  
Baimao Zhong ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Southern China ◽  
Gene Mutations ◽  
Major Hydrophilic Region ◽  
S Gene ◽  
B Virus ◽  
Hydrophilic Region

N-glycosylation mutations within hepatitis B virus surface major hydrophilic region contribute mostly to immune escape

2014 ◽  
Vol 60 (3) ◽  
pp. 515-522 ◽  
Author(s):  
De-Min Yu ◽  
Xin-Hua Li ◽  
Vannary Mom ◽  
Zhong-Hua Lu ◽  
Xiang-Wei Liao ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Immune Escape ◽  
Major Hydrophilic Region ◽  
Virus Surface ◽  
B Virus ◽  
Hydrophilic Region

The prevalence of mutations in the major hydrophilic region of the surface antigen of hepatitis B virus varies with subgenotype

2015 ◽  
Vol 143 (16) ◽  
pp. 3572-3582 ◽  
Author(s):  
X. Y. WANG ◽  
T. J. HARRISON ◽  
X. HE ◽  
Q. Y. CHEN ◽  
G. J. LI ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Cluster Sampling ◽  
Resistance Mutations ◽  
Major Hydrophilic Region ◽  
B Virus ◽  
Hydrophilic Region ◽  
Genotype C ◽  
Asymptomatic Individuals

SUMMARYMutations in the major hydrophilic region (MHR) of the surface antigen of hepatitis B virus (HBV) may result in vaccine escape, failure of immunotherapy and antiviral resistance. These mutants may be transmitted and constitute a public health threat. We aimed to determine the prevalence of MHR mutations of HBV in areas of high endemicity in Guangxi, China. HBV surface gene was analysed from 278 HBsAg-positive asymptomatic individuals recruited from Guangxi using cluster sampling. Three genotypes, B, C and I, were identified. The overall prevalence of MHR mutations is 17·6%. The prevalence of MHR mutations in genotype B (15·1%) is not significantly different from that in genotype C (16·4%). However, the prevalence in subgenotype C5 (31·1%) is significantly higher than in subgenotype C2 (13·0%) (χ2= 6·997,P< 0·05). The prevalence of escape mutations and overlapping polymerase substitutions in subgenotype C5 is significantly higher than in subgenotypes B2 and C2. In total, 7·9% of MHR mutants are escape mutations and 72·1% of MHR mutations produced amino-acid changes in the overlapping polymerase, including resistance mutations to entecavir. Our results suggest that the prevalence of MHR mutations varies with subgenotype. The prevalence of escape mutations and polymerase mutations may be associated with subgenotype.

scholarly journals Ultradeep Sequencing for Detection of Quasispecies Variants in the Major Hydrophilic Region of Hepatitis B Virus in Indonesian Patients

2015 ◽  
Vol 53 (10) ◽  
pp. 3165-3175 ◽  
Author(s):  
Laura Navika Yamani ◽  
Yoshihiko Yano ◽  
Takako Utsumi ◽  
Juniastuti ◽  
Hadi Wandono ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Liver Disease ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Viral Population ◽  
Advanced Liver Disease ◽  
Major Hydrophilic Region ◽  
B Virus ◽  
Hbsag Titer ◽  
Hydrophilic Region

Quasispecies of hepatitis B virus (HBV) with variations in the major hydrophilic region (MHR) of the HBV surface antigen (HBsAg) can evolve during infection, allowing HBV to evade neutralizing antibodies. These escape variants may contribute to chronic infections. In this study, we looked for MHR variants in HBV quasispecies using ultradeep sequencing and evaluated the relationship between these variants and clinical manifestations in infected patients. We enrolled 30 Indonesian patients with hepatitis B infection (11 with chronic hepatitis and 19 with advanced liver disease). The most common subgenotype/subtype of HBV was B3/adw (97%). The HBsAg titer was lower in patients with advanced liver disease than that in patients with chronic hepatitis. The MHR variants were grouped based on the percentage of the viral population affected: major, ≥20% of the total population; intermediate, 5% to <20%; and minor, 1% to <5%. The rates of MHR variation that were present in the major and intermediate viral population were significantly greater in patients with advanced liver disease than those in chronic patients. The most frequent MHR variants related to immune evasion in the major and intermediate populations were P120Q/T, T123A, P127T, Q129H/R, M133L/T, and G145R. The major population of MHR variants causing impaired of HBsAg secretion (e.g., G119R, Q129R, T140I, and G145R) was detected only in advanced liver disease patients. This is the first study to use ultradeep sequencing for the detection of MHR variants of HBV quasispecies in Indonesian patients. We found that a greater number of MHR variations was related to disease severity and reduced likelihood of HBsAg titer.

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