scholarly journals Development and evaluation of one-step multiplex real-time RT-PCR assay for simultaneous detection of Zika virus and Chikungunya virus

2017 ◽  
Vol 90 (3) ◽  
pp. 389-396 ◽  
Author(s):  
Si-Qing Liu ◽  
Xiao Li ◽  
Cheng-Lin Deng ◽  
Zhi-Ming Yuan ◽  
Bo Zhang
Keyword(s):  
Real Time ◽  
Zika Virus ◽  
Chikungunya Virus ◽  
Simultaneous Detection ◽  
Rt Pcr ◽  
Pcr Assay ◽  
One Step

A one-step multiplex real-time RT-PCR assay for rapid and simultaneous detection of human norovirus genogroup I, II and IV

2013 ◽  
Vol 189 (2) ◽  
pp. 277-282 ◽  
Author(s):  
Yong Yan ◽  
Heng-hui Wang ◽  
Lei Gao ◽  
Ji-mei Ji ◽  
Zhi-jie Ge ◽  
...  
Keyword(s):  
Real Time ◽  
Simultaneous Detection ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Human Norovirus ◽  
One Step

scholarly journals Development of a multiplex one-step real-time RT-PCR assay for the simultaneous detection of eight viruses associated with febrile rash illnesses

2020 ◽  
Vol 2 (2) ◽  
pp. 89-94 ◽  
Author(s):  
Aili Cui ◽  
Shulei Wang ◽  
Qiang Zhang ◽  
Huiling Wang ◽  
Zhen Zhu ◽  
...  
Keyword(s):  
Real Time ◽  
Simultaneous Detection ◽  
Rt Pcr ◽  
Pcr Assay ◽  
One Step

Development and evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantification of Chikungunya virus

2007 ◽  
Vol 39 (3) ◽  
pp. 188-193 ◽  
Author(s):  
S.R. Santhosh ◽  
M.M. Parida ◽  
P.K. Dash ◽  
A. Pateriya ◽  
B. Pattnaik ◽  
...  
Keyword(s):  
Real Time ◽  
Chikungunya Virus ◽  
Sybr Green ◽  
Sybr Green I ◽  
Rt Pcr ◽  
Pcr Assay ◽  
One Step ◽  
Detection And Quantification

scholarly journals Surveillance of dengue virus in individual Aedes aegypti mosquitoes collected concurrently with suspected human cases in Tarlac City, Philippines

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Jean Claude Balingit ◽  
Thaddeus M. Carvajal ◽  
Mariko Saito-Obata ◽  
Maribet Gamboa ◽  
Amalea Dulcene Nicolasora ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Vector Control ◽  
Real Time ◽  
Phylogenetic Analyses ◽  
Simultaneous Detection ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Denv Serotypes ◽  
One Step

Abstract Background Vector control measures are critical for the prevention and reduction of dengue virus (DENV) transmission. Effective vector control is reliant not only on knowledge of mosquito abundance, but also on the timely and accurate detection of mosquito-borne infection. Mosquito-based virus surveillance programs typically rely on pool-based mosquito testing, although whether individual-based mosquito testing is a feasible alternative to this has not been widely studied. Applying an individual-based mosquito testing approach, we conducted a 1-month surveillance study of DENV in adult Aedes aegypti mosquitoes in homes of suspected dengue patients during the 2015 peak dengue season in Tarlac City, Philippines to more accurately assess the mosquito infection rate and identify the DENV serotypes and genotypes concurrently co-circulating in mosquitoes and patients there. Methods We performed a one-step multiplex real-time reverse transcription-polymerase chain reaction (RT-PCR) assay for the simultaneous detection and serotyping of DENV in patients and individual female Ae. aegypti mosquitoes. Additionally, we performed sequencing and phylogenetic analyses to further characterize the detected DENV serotypes in mosquitoes and patients at the genotype level. Results We collected a total of 583 adult Ae. aegypti mosquitoes, of which we individually tested 359 female mosquitoes for the presence of DENV. Ten (2.8%) of the 359 female mosquitoes were positive for the presence of DENV. We detected DENV-1, DENV-2, and DENV-4 in the field-collected mosquitoes, which was consistent with the serotypes concurrently found in infected patients. Sequencing and phylogenetic analyses of the detected DENV serotypes based on the partial sequence of the evelope (E) gene revealed three genotypes concurrently present in the sampled mosquitoes and patients during the study period, namely DENV-1 genotype IV, DENV-2 Cosmopolitan genotype, and DENV-4 genotype II. Conclusions We demonstrated the utility of a one-step multiplex real-time RT-PCR assay for the individual-based DENV surveillance of mosquitoes. Our findings reinforce the importance of detecting and monitoring virus activity in local mosquito populations, which are critical for dengue prevention and control.

scholarly journals A35 The first laboratory confirmation of chikungunya outbreak in Ethiopia

2019 ◽  
Vol 5 (Supplement_1) ◽  
Author(s):  
Mesfin Mengesha Tsegaye ◽  
Aadamu Tayachew ◽  
Desalegn Belay ◽  
Abebe Alemu ◽  
Berhane Beyene
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Laboratory Investigation ◽  
Emergency Preparedness ◽  
Zika Virus ◽  
Chikungunya Virus ◽  
Febrile Illness ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Serum Samples

Abstract Chikungunya is a viral disease (genus Alphavirus) which is transmitted to humans by infected mosquitoes—including Aedes aegypti and A. albopictus. An outbreak of febrile illness, suspected to have been caused by chikungunya, was reported in June 2016 from Dolloado district, Suuf Kebele, in the Somalia regional state of Ethiopia that borders the Mandera county of Kenya where a confirmed chikungunya outbreak was ongoing. Laboratory investigation was carried out to confirm if the outbreak in Ethiopia was caused by Chikungunya virus. Ten serum samples were collected from suspected patients visiting a health center in Suuf Kebel, who were then sent to the Nation laboratory in Ethiopian Public Health Institute. RNA was extracted from the serum samples using QIAgene RNA Mini kit, and PCR detection of dengue, chikungunya, and Zika virus nucleic acid was done using Trioplex Real-time RT-PCR Assay following the protocol from the Center for Disease Control (CDC). The Trioplex Real-time RT-PCR assay, for detection and differentiation of RNA from dengue, Chikungunya and Zika, was provided by CDC as part of the zika emergency preparedness effort. Of the nine samples tested, eight (88.88%) were found to be positive for chikungunya virus nucleic acid but negative for dengue and Zika virus nucleic acids. The median age of the affected sampled patients was 40 years, and males appear to be more affected (66.6% of sampled patients). The laboratory investigation confirmed that the outbreak was caused by chikungunya virus. Even though further molecular characterization of the positive isolates will provide more information as to the circulating genotypes and elucidate the origin of the outbreak virus, it is also possible to assume that the outbreak was an extension of the outbreak in neighboring countries in Kenya and, therefore, warrants that cross-border integration efforts to control chikungunya should be implemented by the concerned countries.

scholarly journals A One-Step, Triplex, Real-Time RT-PCR Assay for the Simultaneous Detection of Enterovirus 71, Coxsackie A16 and Pan-Enterovirus in a Single Tube

PLoS ONE ◽  
2014 ◽  
Vol 9 (7) ◽  
pp. e102724 ◽  
Author(s):  
Shiyin Zhang ◽  
Jin Wang ◽  
Qiang Yan ◽  
Shuizhen He ◽  
Wenbin Zhou ◽  
...  
Keyword(s):  
Real Time ◽  
Simultaneous Detection ◽  
Enterovirus 71 ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Single Tube ◽  
One Step
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