Comparison of the performance in detection of HPV infections between the high-risk HPV genotyping real time PCR and the PCR-reverse dot blot assays

2017 ◽  
Vol 90 (1) ◽  
pp. 177-183 ◽  
Author(s):  
Lahong Zhang ◽  
Yibei Dai ◽  
Jiahuan Chen ◽  
Liquan Hong ◽  
Yuhua Liu ◽  
...  
Keyword(s):  
High Risk ◽  
Real Time ◽  
Real Time Pcr ◽  
Dot Blot ◽  
Hpv Genotyping ◽  
High Risk Hpv ◽  
Reverse Dot Blot

APPLICATION OF REAL-TIME PRC TECHNIQUE AND REVERSE DOT-BLOT FOR DETECTION THE HIGH RISK TYPES OF HUMAN PAPILLOMAVIRUS CAUSING CERVICAL CANCER

2017 ◽  
pp. 99-103
Author(s):  
Van Bao Thang Phan ◽  
Hoang Bach Nguyen ◽  
Van Thanh Nguyen ◽  
Thi Nhu Hoa Tran ◽  
Viet Quynh Tram Ngo
Keyword(s):  
Cervical Cancer ◽  
High Risk ◽  
Real Time ◽  
Real Time Pcr ◽  
Dot Blot ◽  
The Real ◽  
Dot Blot Assay ◽  
Hpv Types ◽  
High Risk Hpv ◽  
Reverse Dot Blot

Introduction: Infection with HPV is the main cause of cervical cancer. Determining HPV infection and the types of HPV plays an important role in diagnosis, treatment and prognosis of cervicitis/cervical cancer. Aims: Determining proportion of high-risk HPV types and the occurrence of coinfection with multiple HPV types. Methods: 177 women with cervicitis or abnormal Pap smear result were enrolled in the study. Performing the real-time PCR for detecting HPV and the reverse DOT-BLOT assay for determining type of HPV in cases of positive PCR. Results: 7 types of high-risk HPV was dectected, the majority of these types were HPV type 18 (74.6%) and HPV type 16 (37.6%); the proportion of infection with only one type of HPV was 30.4% and coinfection with multiple HPV types was higher (69.6%), the coinfected cases with 2 and 3 types were dominated (32.2% and 20.3%, respectively) and the coinfected cases with 4 and 5 types were rare. Conclusion: Use of the real-time PCR and reverse DOT-BLOT assay can determine the high-risk HPV types and the occurrence of coinfection with multiple HPV types. Key words: HPV type, Reverse DOT-BLOT, real-time PCR,PCR, cervical cancer

High risk HPV load estimated by Hybrid Capture II® correlates with HPV16 load measured by real-time PCR in cervical smears of HPV16-infected women

2004 ◽  
Vol 31 (2) ◽  
pp. 140-147 ◽  
Author(s):  
Jean-Luc Prétet ◽  
Véronique Dalstein ◽  
Sylvain Monnier-Benoit ◽  
Sébastien Delpeut ◽  
Christiane Mougin
Keyword(s):  
High Risk ◽  
Real Time ◽  
Real Time Pcr ◽  
Hybrid Capture ◽  
Cervical Smears ◽  
High Risk Hpv

scholarly journals Improving Laboratory Efficiency by Automation of Preanalytic Processing of ThinPrep Specimens for Real-Time PCR High-Risk HPV Testing

2016 ◽  
Vol 21 (3) ◽  
pp. 432-438 ◽  
Author(s):  
Daniela Barbieri ◽  
Simona Venturoli ◽  
Silvano Costa ◽  
Maria Paola Landini
Keyword(s):  
High Risk ◽  
Real Time ◽  
Real Time Pcr ◽  
Hpv Testing ◽  
High Risk Hpv
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