Prevalence and molecular characterization of human rhinovirus in stool samples of individuals with and without acute gastroenteritis

2016 ◽  
Vol 89 (5) ◽  
pp. 801-808 ◽  
Author(s):  
Prapaporn Khoonta ◽  
Piyada Linsuwanon ◽  
Nawarat Posuwan ◽  
Sompong Vongpunsawad ◽  
Sunchai Payungporn ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Acute Gastroenteritis ◽  
Human Rhinovirus ◽  
Stool Samples

Surveillance and molecular characterization of human sapovirus in patients with acute gastroenteritis in Brazil, 2010 to 2017

2021 ◽  
pp. 104844
Author(s):  
Audrey Cilli ◽  
Adriana Luchs ◽  
Simone G. Morillo ◽  
Rita de Cassia C. Carmona ◽  
Fabiana C.P. dos Santos ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Acute Gastroenteritis

scholarly journals Molecular Characterization of Giardia duodenalis in Children and Adults Sampled in Algeria

2020 ◽  
Vol 9 (1) ◽  
pp. 54
Author(s):  
Salem Belkessa ◽  
Daniel Thomas-Lopez ◽  
Karim Houali ◽  
Farida Ghalmi ◽  
Christen Rune Stensvold
Keyword(s):  
Real Time ◽  
Molecular Characterization ◽  
Real Time Pcr ◽  
Intestinal Parasites ◽  
Giardia Duodenalis ◽  
Specific Pcr ◽  
Stool Samples ◽  
Pcr Targeting ◽  
Assemblage B

The molecular epidemiology of giardiasis in Africa remains unclear. A study was carried out across four hospitals in Algeria. A total of 119 fecal samples from 55 children, 37 adults, and 27 individuals of undetermined age, all scored positive for intestinal parasites by microscopy, and were screened by real-time PCR for Giardia. Molecular characterization of Giardia was performed by assemblage-specific PCR and PCR targeting the triose phosphate isomerase gene (tpi). Of the 119 samples, 80 (67%) were Giardia-positive by real-time PCR. For 48 moderately-highly real-time PCR-positive samples, tpi genotyping assigned 22 samples to Assemblage A and 26 to Assemblage B. Contrary to Assemblage A, Assemblage B exhibited substantial genetic diversity and allelic heterozygosity. Assemblage-specific PCR proved to be specific for discriminating Assemblage A or B but not as sensitive as tpi genotyping. We confirmed that real-time PCR is more sensitive than microscopy for detecting Giardia in stool samples and that robust amplification and sequencing of the tpi gene is feasible when moderate-to-strongly real-time PCR-positive samples are used. This study is one of the few performed in Africa providing genotyping data on Giardia infections in humans. Both assemblages A and B were commonly seen and not associated with specific sociodemographic data.

scholarly journals Molecular characterization of norovirus from acute gastroenteritis patients in Malaysia

2011 ◽  
Vol 5 (18) ◽  
pp. 2733-2736 ◽  
Author(s):  
Zuridah H ◽  
Hadi M Sufiyan ◽  
K Teh L ◽  
H Zed Zakari A
Keyword(s):  
Molecular Characterization ◽  
Acute Gastroenteritis

scholarly journals Molecular characterization of hookworm spp. isolated from food handlers, Khartoum, Sudan: A cross-sectional study

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 662
Author(s):  
Tarig A. Gamar ◽  
Hassan H. Musa ◽  
Hisham N. Altayb ◽  
Mohamed H. Mohamed ◽  
Adam D. Abakar
Keyword(s):  
Public Health ◽  
Molecular Characterization ◽  
Molecular Probe ◽  
Hookworm Infection ◽  
Food Handlers ◽  
The Public ◽  
Necator Americanus ◽  
Link Type ◽  
Stool Samples

Background: Hookworms infect the intestines, cause an itchy rash, respiratory and gastrointestinal problems, and eventually iron deficiency (anaemia) due to the ongoing loss of blood. The objectives of this study were to assess the prevalence and molecular characterization of hookworms isolated from food handlers attending the Public Health Laboratories in Khartoum state, Sudan, for annual check-ups, and to assess the efficiency of PCR as molecular probe for hookworm infection. Methods: A total of 350 foods handlers’ participant's stool samples who were not suspected to be infected with hookworms were studied. Conventional methods were applied to make an early diagnosis. Stool samples were collected from public health laboratories (the public health lab in the Medical Commission) of Khartoum State; Omdurman locality, Khartoum North locality and Khartoum locality between October 2016 and April 2017. Specific identification was made by PCR on specimens identified as positive by Baermann’s technique, which were then sequence and genotyped Results: The prevalence of hookworms in the stool samples of food-handlers was 1.43%. One larval specimen recovered by Baermann’s technique was confirmed to be Necator americanus by PCR. PCR also confirmed that Necator americanus was the common species isolated from four further specimens. The results of DNA sequencing for Necator americanus were deposited in NCBI GenBank under the following accession numbers: sample 91, MH035824; sample 92, MH035825; sample 294, MH035826; and sample 319 MH035827. Conclusion: PCR was found to be effective for confirmation of the diagnosis of hookworm infection and can aid the clinician in initiating prompt and appropriate antiparasite therapy.

scholarly journals Molecular characterization of human rhinovirus in Portugal: 2014–2015 season

2016 ◽  
Vol 82 ◽  
pp. S1-S2
Author(s):  
P. Cristóvão ◽  
P. Pechirra ◽  
P. Conde ◽  
I. Costa ◽  
R. Guiomar
Keyword(s):  
Molecular Characterization ◽  
Human Rhinovirus

Detection and molecular characterization of noroviruses and sapoviruses in children admitted to hospital with acute gastroenteritis in Vietnam

2011 ◽  
Vol 84 (2) ◽  
pp. 290-297 ◽  
Author(s):  
Nguyen V. Trang ◽  
Le T. Luan ◽  
Le T. Kim-Anh ◽  
Vu T.B. Hau ◽  
Le T.H. Nhung ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Acute Gastroenteritis

Molecular characterization of norovirus GII.17 detected in healthy adult, intussusception patient, and acute gastroenteritis children in Thailand

2016 ◽  
Vol 44 ◽  
pp. 330-333 ◽  
Author(s):  
Pattara Khamrin ◽  
Kattareeya Kumthip ◽  
Arpaporn Yodmeeklin ◽  
Kanittapon Supadej ◽  
Nuthapong Ukarapol ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Acute Gastroenteritis ◽  
Healthy Adult ◽  
Adult Intussusception ◽  
Norovirus Gii

Molecular characterization of rotaviruses, noroviruses, sapovirus, and adenoviruses in patients with acute gastroenteritis in Thailand

2009 ◽  
Vol 81 (2) ◽  
pp. 345-353 ◽  
Author(s):  
Leera Kittigul ◽  
Kannika Pombubpa ◽  
Yuthana Taweekate ◽  
Thanapat Yeephoo ◽  
Pattara Khamrin ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Acute Gastroenteritis
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