Identification of IgH gene rearrangement and immunophenotype in an animal model of Epstein-Barr virus-associated lymphomas

2016 ◽  
Vol 88 (10) ◽  
pp. 1804-1813 ◽  
Author(s):  
Yang Zhang ◽  
Xueqin Peng ◽  
Yunlian Tang ◽  
Xiaoning Gan ◽  
Chengkun Wang ◽  
...  
Keyword(s):  
Animal Model ◽  
Epstein Barr Virus ◽  
Gene Rearrangement ◽  
Barr Virus ◽  
Igh Gene Rearrangement ◽  
Epstein Barr

CONFIRMATION OF THE HETEROGENEITY OF CELL PROLIFERATIONS1 BY IMMUNOGLOBULIN GENE REARRANGEMENT ANALYSES POSTTRANSPLANT EPSTEIN-BARR VIRUS-ASSOCIATED B

1989 ◽  
Vol 47 (3) ◽  
pp. 458-464 ◽  
Author(s):  
DOUGLAS W. HANTO ◽  
MARK BIRKENBACH ◽  
GLAUCO FRIZZERA ◽  
KAZIMIERA J. GAJL-PECZALSKA ◽  
RICHARD L. SIMMONS ◽  
...  
Keyword(s):  
Epstein Barr Virus ◽  
Gene Rearrangement ◽  
Immunoglobulin Gene ◽  
Immunoglobulin Gene Rearrangement ◽  
Barr Virus ◽  
Epstein Barr

scholarly journals Spontaneous Epstein-Barr virus transformed B-cell line sharing the identical immunoglobulin gene rearrangement with acute myeloid leukemia

Blood ◽  
1989 ◽  
Vol 73 (3) ◽  
pp. 684-687 ◽  
Author(s):  
HH Gerhartz ◽  
CR Bartram ◽  
A Raghavachar ◽  
H Schmetzer ◽  
C Clemm ◽  
...  
Keyword(s):  
Acute Myeloid Leukemia ◽  
B Cell ◽  
Cell Line ◽  
Heavy Chain ◽  
Myeloid Leukemia ◽  
Epstein Barr Virus ◽  
Gene Rearrangement ◽  
Barr Virus ◽  
Epstein Barr ◽  
Acute Myeloid

Abstract Mononuclear cells from a 44-year-old patient with acute myeloid leukemia (AML) gave rise to a spontaneous permanent cell line cultured in suspension. The cell line was shown to be positive for Epstein-Barr virus nuclear antigen (EBNA). As expected, its composite phenotype was of B-cell type with B-cell antigens (CD 20, CD 21) and with monoclonal surface IgM of kappa type, but without detectable IgM secretion. Surprisingly, identical monoclonal rearrangements of the immunoglobulin heavy chain (JH) sequences could be demonstrated in the uncultured bone marrow AML cells and in the cell line that also had kappa light chain gene rearrangement. This is the first case to our knowledge of an EBNA positive B-cell line with identical monoclonal Ig heavy chain rearrangement as detected in myeloblastic leukemia cells.

scholarly journals Epstein-Barr virus (EBV)-carrying and -expressing T-cell lines established from severe chronic active EBV infection

Blood ◽  
1996 ◽  
Vol 87 (4) ◽  
pp. 1446-1457 ◽  
Author(s):  
S Imai ◽  
M Sugiura ◽  
O Oikawa ◽  
S Koizumi ◽  
M Hirao ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Cell Lines ◽  
Epstein Barr Virus ◽  
Gene Rearrangement ◽  
Barr Virus ◽  
Ebv Infection ◽  
Epstein Barr ◽  
T Cell Lines

Four novel Epstein-Barr virus (EBV)-carrying T-cell lines, designated SIS, AIK-T8, AIK-T4, and SKN, were established from peripheral blood lymphocytes (PBL) of patients with severe chronic active EBV infection, in the presence of interleukin-2 and 4-deoxyphorbol ester. AIK-T8 and - T4 were derived from a single patient. Cell marker and genotype analyses showed that SIS, AIK-T8, and AIK-T4 had mature T-cell phenotypes with clonally rearranged T-cell receptor (TCR) genes, whereas SKN had an immature T-cell phenotype without TCR gene rearrangement. None of the cell lines expressed B, natural killer, or myeloid antigens or had Ig gene rearrangement. All lines carried EBV genomes in a single episomal form. SIS, AIK-T8, and SKN showed the same phenotype, TCR gene configuration, and/or EBV clonotype as their source or biopsied materials; therefore, they represented EBV-infected T cells proliferating in the patients. TCR gene and EBV episomal structures similar to those of AIK-T4 were not found in its source PBL, probably due to the few parental clones in vivo. All lines expressed EBV-encoded small RNA (EBER) 1, nuclear antigen (EBNA) 1, and latent membrane protein (LMP) 1, -2A, and -2B, but not other EBNAs that could be recognized by EBV-specific immune T cells. EBV replicative antigens were rarely expressed or induced. Such EBV latency reflects the in vivo situation, in which the T cells may evade immune surveillance and be insensitive to antiherpesvirus drugs. Collectively, the data suggest that EBV can target and latently infect T cells at any stage of differentiation in vivo, thus potentially causing uncontrolled T-cell proliferation. These cell lines will facilitate further analyses of possible EBV-induced oncogenicity in T cells.

MLL-AF4 Gene Rearrangement in a Child With Epstein-Barr Virus???Related Posttransplant B-Cell Lymphoma

2003 ◽  
Vol 25 (9) ◽  
pp. 740-742 ◽  
Author(s):  
Funda ??orap??io??lu ◽  
Nur Olgun ◽  
Faik Sarialio??lu ◽  
Kamer Mutafo??lu Uysal ◽  
Hale ??ren ◽  
...  
Keyword(s):  
B Cell ◽  
Epstein Barr Virus ◽  
Gene Rearrangement ◽  
Cell Lymphoma ◽  
B Cell Lymphoma ◽  
Barr Virus ◽  
Epstein Barr

scholarly journals Complete Nucleotide Sequence of the Rhesus Lymphocryptovirus: Genetic Validation for an Epstein-Barr Virus Animal Model

2002 ◽  
Vol 76 (1) ◽  
pp. 421-426 ◽  
Author(s):  
Pierre Rivailler ◽  
Hua Jiang ◽  
Young-gyu Cho ◽  
Carol Quink ◽  
Fred Wang
Keyword(s):  
Animal Model ◽  
Epstein Barr Virus ◽  
Interleukin 10 ◽  
Open Reading Frames ◽  
Gene Repertoire ◽  
Barr Virus ◽  
Viral Glycoproteins ◽  
Epstein Barr ◽  
Stimulating Factor ◽  
Reading Frames

ABSTRACT We sequenced the rhesus lymphocryptovirus (LCV) genome in order to determine its genetic similarity to Epstein-Barr virus (EBV). The rhesus LCV encodes a repertoire identical to that of EBV, with 80 open reading frames, including cellular interleukin-10, bcl-2, and colony-stimulating factor 1 receptor homologues and an equivalent set of viral glycoproteins. The highly conserved rhesus LCV gene repertoire provides a unique animal model for the study of EBV pathogenesis.

Sign in / Sign up

Export Citation Format

Share Document