Transcriptome profiling reveals differential expression of interferon family induced by dengue virus 2 in human endothelial cells on tissue culture plastic and polyacrylamide hydrogel

2016 ◽  
Vol 88 (7) ◽  
pp. 1137-1151 ◽  
Author(s):  
Hua Pei ◽  
Li Zuo ◽  
Jing Ma ◽  
Lili Cui ◽  
Fangfang Yu ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Endothelial Cells ◽  
Dengue Virus ◽  
Differential Expression ◽  
Transcriptome Profiling ◽  
Polyacrylamide Hydrogel ◽  
Human Endothelial Cells ◽  
Tissue Culture Plastic

scholarly journals The Ubiquitin−Proteasome Pathway Is Important for Dengue Virus Infection in Primary Human Endothelial Cells

2010 ◽  
Vol 9 (10) ◽  
pp. 4960-4971 ◽  
Author(s):  
Rattiyaporn Kanlaya ◽  
Sa-nga Pattanakitsakul ◽  
Supachok Sinchaikul ◽  
Shui-Tein Chen ◽  
Visith Thongboonkerd
Keyword(s):  
Endothelial Cells ◽  
Virus Infection ◽  
Dengue Virus ◽  
Human Endothelial Cells ◽  
Dengue Virus Infection ◽  
Ubiquitin Proteasome Pathway ◽  
Ubiquitin Proteasome ◽  
Proteasome Pathway

Human Endothelial Cells in Tissue Culture

1980 ◽  
Vol 168 (1-3) ◽  
pp. 213-214 ◽  
Author(s):  
P. Oboril ◽  
Ch. Mittermayer
Keyword(s):  
Tissue Culture ◽  
Endothelial Cells ◽  
Human Endothelial Cells

scholarly journals αvβ3Integrin Mediates the Cell-adhesive Capacity and Biological Activity of Basic Fibroblast Growth Factor (FGF-2) in Cultured Endothelial Cells

1997 ◽  
Vol 8 (12) ◽  
pp. 2449-2461 ◽  
Author(s):  
Marco Rusnati ◽  
Elena Tanghetti ◽  
Patrizia Dell’Era ◽  
Anna Gualandris ◽  
Marco Presta
Keyword(s):  
Tissue Culture ◽  
Endothelial Cells ◽  
Cell Adhesion ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Amino Acid Sequences ◽  
Dependent Manner ◽  
Fibroblast Growth ◽  
Tissue Culture Plastic ◽  
Cell Adhesive

Fibroblast growth factor-2 (FGF-2) immobilized on non-tissue culture plastic promotes adhesion and spreading of bovine and human endothelial cells that are inhibited by anti-FGF-2 antibody. Heat-inactivated FGF-2 retains its cell-adhesive activity despite its incapacity to bind to tyrosine-kinase FGF receptors or to cell-surface heparan sulfate proteoglycans. Recombinant glutathione-S-transferase-FGF-2 chimeras and synthetic FGF-2 fragments identify two cell-adhesive domains in FGF-2 corresponding to amino acid sequences 38–61 and 82–101. Both regions are distinct from the FGF-receptor-binding domain of FGF-2 and contain a DGR sequence that is the inverse of the RGD cell-recognition sequence. Calcium deprivation, RGD-containing eptapeptides, soluble vitronectin (VN), but not fibronectin (FN), inhibit cell adhesion to FGF-2. Conversely, soluble FGF-2 prevents cell adhesion to VN but not FN, thus implicating VN receptor in the cell-adhesive activity of FGF-2. Accordingly, monoclonal and polyclonal anti-αvβ3antibodies prevent cell adhesion to FGF-2. Also, purified human αvβ3binds to immobilized FGF-2 in a cation-dependent manner, and this interaction is competed by soluble VN but not by soluble FN. Finally, anti-αvβ3monoclonal and polyclonal antibodies specifically inhibit mitogenesis and urokinase-type plasminogen activator (uPA) up-regulation induced by free FGF-2 in endothelial cells adherent to tissue culture plastic. These data demonstrate that FGF-2 interacts with αvβ3integrin and that this interaction mediates the capacity of the angiogenic growth factor to induce cell adhesion, mitogenesis, and uPA up-regulation in endothelial cells.

scholarly journals A Substrate-Mimicking Basement Membrane Drives the Organization of Human Mesenchymal Stromal Cells and Endothelial Cells Into Perivascular Niche-Like Structures

2021 ◽  
Vol 9 ◽  
Author(s):  
Valeria Perugini ◽  
Matteo Santin
Keyword(s):  
Bone Marrow ◽  
Tissue Culture ◽  
Endothelial Cells ◽  
Stem Cell ◽  
Stromal Cells ◽  
Stem Cell Niche ◽  
Tissue Culture Plastic ◽  
Histological Features ◽  
Cell Niche ◽  
3D Spheroids

Extracellular matrix-derived products (e.g. Matrigel) are widely used for in vitro cell cultures both as two-dimensional (2D) substrates and as three-dimensional (3D) encapsulation gels because of their ability to control cell phenotypes through biospecific cues. However, batch-to-batch variations, poor stability, cumbersome handling, and the relatively high costs strictly limit their use. Recently, a new substrate known as PhenoDrive-Y has been used as 2D coating of tissue culture plastic showing to direct the bone marrow mesenchymal stromal cells (MSCs) toward the formation of 3D spheroids. When organized into 3D spheroids, the MSCs expressed levels of pluripotency markers and of paracrine angiogenic activity higher than those of the MSCs adhering as fibroblast-like colonies on tissue culture plastic. The formation of the spheroids was attributed to the properties of this biomaterial that resemble the main features of the basement membrane by mimicking the mesh structure of collagen IV and by presenting the cells with orderly spaced laminin bioligands. In this study, PhenoDrive-Y was compared to Matrigel for its ability to drive the formation of perivascular stem cell niche-like structures in 2D co-culture conditions of human endothelial cells and adult bone marrow MSCs. Morphological analyses demonstrated that, when compared to Matrigel, PhenoDrive-Y led endothelial cells to sprout into a more consolidated tubular network and that the MSCs nestled as compact spheroids above the anastomotic areas of this network resemble more closely the histological features of the perivascular stem cell niche. A study of the expressions of relevant markers led to the identification of the pathways linking the PhenoDrive-Y biomimicking properties to the acquired histological features, demonstrating the enhanced levels of stemness, renewal potential, predisposition to migration, and paracrine activities of the MSCs.

Tissue plasminogen activator induced by dengue virus infection of human endothelial cells

2003 ◽  
Vol 70 (4) ◽  
pp. 610-616 ◽  
Author(s):  
Ying-Huey Huang ◽  
Huan-Yao Lei ◽  
Hsiao-Sheng Liu ◽  
Yee-Shin Lin ◽  
Shun-Hua Chen ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Virus Infection ◽  
Dengue Virus ◽  
Plasminogen Activator ◽  
Tissue Plasminogen Activator ◽  
Human Endothelial Cells ◽  
Dengue Virus Infection ◽  
Tissue Plasminogen

Dengue virus induces thrombomodulin expression in human endothelial cells and monocytes in vitro

2009 ◽  
Vol 58 (5) ◽  
pp. 368-374 ◽  
Author(s):  
Lien-Cheng Chen ◽  
Huey-Wen Shyu ◽  
Hui-Min Lin ◽  
Huan-Yao Lei ◽  
Yee-Shin Lin ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Dengue Virus ◽  
Human Endothelial Cells
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