Detection of human bocavirus in nasopharyngeal aspirates versus in broncho-alveolar lavage fluids in children with lower respiratory tract infections

2015 ◽  
Vol 88 (2) ◽  
pp. 211-215 ◽  
Author(s):  
Ling Li ◽  
Tian Zhu ◽  
Zheng-Rong Chen ◽  
Yong-Dong Yan ◽  
Li-Ping He ◽  
...  
Keyword(s):  
Respiratory Tract ◽  
Lower Respiratory Tract ◽  
Respiratory Tract Infections ◽  
Human Bocavirus ◽  
Lower Respiratory Tract Infections ◽  
Tract Infections

scholarly journals Evidence of Prior Exposure to Human Bocavirus as Determined by a Retrospective Serological Study of 404 Serum Samples from Adults in the United States

2009 ◽  
Vol 16 (5) ◽  
pp. 597-604 ◽  
Author(s):  
Sylvain Cecchini ◽  
Alejandro Negrete ◽  
Tamas Virag ◽  
Barney S. Graham ◽  
Jeffrey I. Cohen ◽  
...  
Keyword(s):  
Respiratory Tract ◽  
Human Serum ◽  
Lower Respiratory Tract ◽  
Respiratory Tract Infections ◽  
The United States ◽  
Capsid Proteins ◽  
Human Bocavirus ◽  
Lower Respiratory Tract Infections ◽  
Serum Samples ◽  
Tract Infections

ABSTRACT Recently, molecular screening for pathogenic agents has identified a partial genome of a novel parvovirus, called human bocavirus (HBoV). The presence of this newly described parvovirus correlated with upper and lower respiratory tract infections in children. Lower respiratory tract infections are a leading cause of hospital admission in children, and the etiological agent has not been identified in up to 39% of these cases. Using baculovirus expression vectors (BEVs) and an insect cell system, we produced virus-like particles (VLPs) of HBoV. The engineered BEVs express the HBoV capsid proteins stoichiometrically from a single open reading frame. Three capsid proteins assemble into the VLP rather than two proteins predicted from the HBoV genome sequence. The denatured capsid proteins VP1, VP2, and VP3 resolve on silver-stained sodium dodecyl sulfate-polyacrylamide gels as three bands with apparent molecular masses of 72 kDa, 68 kDa, and 62 kDa, respectively. VP2 apparently initiates at a GCT codon (alanine) 273 nucleotides downstream from the VP1 start site and 114 nucleotides upstream from the VP3 initiation site. We characterized the stable capsids using physical, biochemical, and serological techniques. We found that the density of the VLP is 1.32 g/cm3 and is consistent with an icosahedral symmetry with approximately a 25-nm diameter. Rabbit antiserum against the capsid of HBoV, which did not cross-react with adeno-associated virus type 2, was used to develop enzyme-linked immunosorbent assays (ELISAs) for anti-HBoV antibodies in human serum. Using ELISA, we tested 404 human serum samples and established a range of antibody titers in a large U.S. adult population sample.

Clinical and epidemiologic profile of lower respiratory tract infections associated with human bocavirus

2013 ◽  
Vol 48 (11) ◽  
pp. 1112-1118 ◽  
Author(s):  
Cristiano do Amaral de Leon ◽  
Sérgio Luis Amantea ◽  
Diogo André Pilger ◽  
Vlademir Cantarelli
Keyword(s):  
Respiratory Tract ◽  
Lower Respiratory Tract ◽  
Respiratory Tract Infections ◽  
Human Bocavirus ◽  
Lower Respiratory Tract Infections ◽  
Epidemiologic Profile ◽  
Tract Infections

scholarly journals Human bocavirus found in children with lower respiratory tract infection

2005 ◽  
Vol 10 (34) ◽  
Author(s):  
Collective Editorial team
Keyword(s):  
Tract Infection ◽  
Respiratory Tract ◽  
Respiratory Tract Infection ◽  
Lower Respiratory Tract Infection ◽  
Lower Respiratory Tract ◽  
Respiratory Tract Infections ◽  
Human Bocavirus ◽  
Lower Respiratory Tract Infections ◽  
Tract Infections ◽  
First Time

A new parvovirus which causes lower respiratory tract infections in children has been identified for the first time, and has provisionally been called human bocavirus

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