scholarly journals Benefits and drawbacks of molecular techniques for diagnosis of viral respiratory infections. Experience with two multiplex PCR assays

2015 ◽  
Vol 88 (1) ◽  
pp. 45-50 ◽  
Author(s):  
Laura García-Arroyo ◽  
Núria Prim ◽  
Neus Martí ◽  
Maria Carme Roig ◽  
Ferran Navarro ◽  
...  
2016 ◽  
Vol 144 (10) ◽  
pp. 2064-2076 ◽  
Author(s):  
S. NICKBAKHSH ◽  
F. THORBURN ◽  
B. VON WISSMANN ◽  
J. McMENAMIN ◽  
R. N. GUNSON ◽  
...  

SUMMARYViral respiratory infections continue to pose a major global healthcare burden. At the community level, the co-circulation of respiratory viruses is common and yet studies generally focus on single aetiologies. We conducted the first comprehensive epidemiological analysis to encompass all major respiratory viruses in a single population. Using extensive multiplex PCR diagnostic data generated by the largest NHS board in Scotland, we analysed 44230 patient episodes of respiratory illness that were simultaneously tested for 11 virus groups between 2005 and 2013, spanning the 2009 influenza A pandemic. We measured viral infection prevalence, described co-infections, and identified factors independently associated with viral infection using multivariable logistic regression. Our study provides baseline measures and reveals new insights that will direct future research into the epidemiological consequences of virus co-circulation. In particular, our study shows that (i) human coronavirus infections are more common during influenza seasons and in co-infections than previously recognized, (ii) factors associated with co-infection differ from those associated with viral infection overall, (iii) virus prevalence has increased over time especially in infants aged <1 year, and (iv) viral infection risk is greater in the post-2009 pandemic era, likely reflecting a widespread change in the viral population that warrants further investigation.


Author(s):  
Valentin Sencio ◽  
Marina Gomes Machado ◽  
François Trottein

AbstractBacteria that colonize the human gastrointestinal tract are essential for good health. The gut microbiota has a critical role in pulmonary immunity and host’s defense against viral respiratory infections. The gut microbiota’s composition and function can be profoundly affected in many disease settings, including acute infections, and these changes can aggravate the severity of the disease. Here, we discuss mechanisms by which the gut microbiota arms the lung to control viral respiratory infections. We summarize the impact of viral respiratory infections on the gut microbiota and discuss the potential mechanisms leading to alterations of gut microbiota’s composition and functions. We also discuss the effects of gut microbial imbalance on disease outcomes, including gastrointestinal disorders and secondary bacterial infections. Lastly, we discuss the potential role of the lung–gut axis in coronavirus disease 2019.


Author(s):  
Sinha Pranay ◽  
Katherine Reifler ◽  
Michael Rossi ◽  
Manish Sagar

Abstract Detection of diverse respiratory viruses in Boston was around 80% lower after practices were instituted to limit COVID-19 spread compared to the same time period during the previous five years. Continuing the strategies that lower COVID-19 dissemination may be useful in decreasing the incidence of other viral respiratory infections.


2016 ◽  
Vol 35 (10) ◽  
pp. 1057-1061 ◽  
Author(s):  
Charlotte Buehler Cherry ◽  
Marie R. Griffin ◽  
Kathryn M. Edwards ◽  
John V. Williams ◽  
Ana I. Gil ◽  
...  

2018 ◽  
pp. e2024 ◽  
Author(s):  
Riffat Naz ◽  
Asma Gul ◽  
Urooj Javed ◽  
Alina Urooj ◽  
Sidra Amin ◽  
...  

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