Use of a commercial ELISA for the detection of measles-specific immunoglobulin G (IgG) in dried blood spots collected from children living in low-resource settings

2015 ◽  
Vol 87 (9) ◽  
pp. 1491-1499 ◽  
Author(s):  
K.Ellicott Colson ◽  
Alan Potter ◽  
Carlos Conde-Glez ◽  
Bernardo Hernandez ◽  
Diego Ríos-Zertuche ◽  
...  
Keyword(s):  
Immunoglobulin G ◽  
Dried Blood Spots ◽  
Low Resource Settings ◽  
Low Resource ◽  
Blood Spots ◽  
Specific Immunoglobulin

Dried Blood Spots Improve Access to HIV Diagnosis and Care for Infants in Low-Resource Settings

2005 ◽  
Vol 38 (5) ◽  
pp. 615-617 ◽  
Author(s):  
Gayle G Sherman ◽  
Gwynneth Stevens ◽  
Stephanie A Jones ◽  
Pamela Horsfield ◽  
Wendy S Stevens
Keyword(s):  
Dried Blood Spots ◽  
Hiv Diagnosis ◽  
Low Resource Settings ◽  
Low Resource ◽  
Blood Spots ◽  
Improve Access

scholarly journals IP-10 dried blood spots assay monitoring treatment efficacy in extrapulmonary tuberculosis in a low-resource setting

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Ida Marie Hoel ◽  
Melissa Davidsen Jørstad ◽  
Msafiri Marijani ◽  
Morten Ruhwald ◽  
Tehmina Mustafa ◽  
...  
Keyword(s):  
Treatment Efficacy ◽  
Extrapulmonary Tuberculosis ◽  
Dried Blood Spots ◽  
Low Resource ◽  
Blood Spots ◽  
Resource Setting ◽  
Low Resource Setting ◽  
Monitoring Treatment

N‐glycosylation analysis of mouse immunoglobulin G isolated from dried blood spots

2020 ◽  
Author(s):  
Anne‐Marie Patenaude ◽  
Julija Erhardt ◽  
René Hennig ◽  
Erdmann Rapp ◽  
Gordan Lauc ◽  
...  
Keyword(s):  
Immunoglobulin G ◽  
Dried Blood Spots ◽  
Blood Spots ◽  
Mouse Immunoglobulin

scholarly journals Dried Blood Spots versus Sera for Detection of Rubella Virus-Specific Immunoglobulin M (IgM) and IgG in Samples Collected during a Rubella Outbreak in Peru

2007 ◽  
Vol 14 (11) ◽  
pp. 1522-1525 ◽  
Author(s):  
Rita F. Helfand ◽  
Cesar Cabezas ◽  
Emily Abernathy ◽  
Carlos Castillo-Solorzano ◽  
Ana Cecilia Ortiz ◽  
...  
Keyword(s):  
Filter Paper ◽  
Rubella Virus ◽  
Dried Blood Spots ◽  
Immunoglobulin M ◽  
Dried Blood Spot ◽  
Blood Spots ◽  
Specific Immunoglobulin ◽  
Blood Spot

ABSTRACT Most persons with rubella virus-specific immunoglobulin M (IgM)- or IgG-positive sera tested positive (98% [n = 178] and 99% [n = 221], respectively) using paired filter paper dried blood spot (DBS) samples, provided that DBS indeterminate results were called positive. For persons with IgM- or IgG-negative sera, 97% and 98%, respectively, were negative using DBS.

scholarly journals Metabolic gestational age assessment in low resource settings: a validation protocol

2021 ◽  
Vol 4 ◽  
pp. 150
Author(s):  
A. Brianne Bota ◽  
Victoria Ward ◽  
Stephen Hawken ◽  
Lindsay A. Wilson ◽  
Monica Lamoureux ◽  
...  
Keyword(s):  
Preterm Birth ◽  
Newborn Screening ◽  
Gestational Age ◽  
Screening Program ◽  
Neonatal Morbidity ◽  
Dried Blood Spots ◽  
Primary Objective ◽  
Low Resource Settings ◽  
Low Resource ◽  
Age Estimates

Preterm birth is the leading global cause of neonatal morbidity and mortality. Reliable gestational age estimates are useful for quantifying population burdens of preterm birth and informing allocation of resources to address the problem. However, evaluating gestational age in low-resource settings can be challenging, particularly in places where access to ultrasound is limited. Our group has developed an algorithm using newborn screening analyte values derived from dried blood spots from newborns born in Ontario, Canada for estimating gestational age within one to two weeks. The primary objective of this study is to validate a program that derives gestational age estimates from dried blood spot samples (heel-prick or cord blood) collected from health and demographic surveillance sites and population representative health facilities in low-resource settings in Zambia, Kenya, Bangladesh and Zimbabwe. We will also pilot the use of an algorithm to identify birth percentiles based on gestational age estimates and weight to identify small for gestational age infants. Once collected from local sites, samples will be tested by the Newborn Screening Ontario laboratory at the Children’s Hospital of Eastern Ontario (CHEO) in Ottawa, Canada. Analyte values will be obtained through laboratory analysis for estimation of gestational age as well as screening for other diseases routinely conducted at Ontario’s newborn screening program. For select conditions, abnormal screening results will be reported back to the sites in real time to facilitate counseling and future clinical management. We will determine the accuracy of our existing algorithm for estimation of gestational age in these newborn samples. Results from this research hold the potential to create a feasible method to assess gestational age at birth in low- and middle-income countries where reliable estimation may be otherwise unavailable.

Comprehensive N-glycosylation analysis of immunoglobulin G from dried blood spots

Glycobiology ◽  
2019 ◽  
Vol 29 (12) ◽  
pp. 817-821 ◽  
Author(s):  
Jelena Simunovic ◽  
Marija Vilaj ◽  
Irena Trbojevic-Akmacic ◽  
Ana Momcilovic ◽  
Frano Vuckovic ◽  
...  
Keyword(s):  
Immunoglobulin G ◽  
Dried Blood Spots ◽  
Ultra Performance Liquid Chromatography ◽  
Storage Conditions ◽  
Sampling Procedure ◽  
Laboratory Equipment ◽  
Total N ◽  
Blood Spots ◽  
Stable Source ◽  
Analytical Variation

Abstract Immunoglobulin G (IgG) glycans are emerging as a new putative biomarker for biological age and different diseases, requiring a robust workflow for IgG glycome analysis, ideally beginning with a simple and undemanding sampling procedure. Here, we report the first comprehensive study on total N-glycans of IgG isolated from dried blood spots (DBSs), which was performed in a high-throughput mode. We compared the IgG N-glycan profiles originating from DBS with those originating from plasma, compared different media for DBS collection, evaluated analytical variation and assessed IgG N-glycan profile stability for different storage conditions. In conclusion, we show that DBSs are a good and stable source material for a robust IgG N-glycan analysis by ultra-performance liquid chromatography, suitable for blood sampling in conditions where no trained personnel and necessary laboratory equipment are available.

scholarly journals Metabolic gestational age assessment in low resource settings: a validation protocol

2020 ◽  
Vol 4 ◽  
pp. 150
Author(s):  
A. Brianne Bota ◽  
Victoria Ward ◽  
Stephen Hawken ◽  
Lindsay A. Wilson ◽  
Monica Lamoureux ◽  
...  
Keyword(s):  
Preterm Birth ◽  
Newborn Screening ◽  
Gestational Age ◽  
Screening Program ◽  
Neonatal Morbidity ◽  
Dried Blood Spots ◽  
Primary Objective ◽  
Low Resource Settings ◽  
Low Resource ◽  
Age Estimates

Preterm birth is the leading global cause of neonatal morbidity and mortality. Reliable gestational age estimates are useful for quantifying population burdens of preterm birth and informing allocation of resources to address the problem. However, evaluating gestational age in low-resource settings can be challenging, particularly in places where access to ultrasound is limited. Our group has developed an algorithm using newborn screening analyte values derived from dried blood spots from newborns born in Ontario, Canada for estimating gestational age within one to two weeks. The primary objective of this study is to validate a program that derives gestational age estimates from dried blood spot samples (heel-prick or cord blood) collected from health and demographic surveillance sites and population representative health facilities in low-resource settings in Zambia, Kenya, Bangladesh and Zimbabwe. We will also pilot the use of an algorithm to identify birth percentiles based on gestational age estimates and weight to identify small for gestational age infants. Once collected from local sites, samples will be tested by the Newborn Screening Ontario laboratory at the Children’s Hospital of Eastern Ontario (CHEO) in Ottawa, Canada. Analyte values will be obtained through laboratory analysis for estimation of gestational age as well as screening for other diseases routinely conducted at Ontario’s newborn screening program. For select conditions, abnormal screening results will be reported back to the sites in real time to facilitate counseling and future clinical management. We will determine the accuracy of our existing algorithm for estimation of gestational age in these newborn samples. Results from this research hold the potential to create a feasible method to assess gestational age at birth in low- and middle-income countries where reliable estimation may be otherwise unavailable.

Use of Protein a Conjugated to Peroxidase to Localize Immunoglobulin G in SSPE Ferret Brain

1982 ◽  
Vol 40 ◽  
pp. 350-351
Author(s):  
Hannah R. Brown ◽  
Anthony F. Nostro ◽  
Halldor Thormar
Keyword(s):  
Immunoglobulin G ◽  
Human Disease ◽  
Measles Virus ◽  
Subacute Sclerosing Panencephalitis ◽  
Protein A ◽  
Inflammatory Lesions ◽  
Sspe Virus ◽  
Specific Immunoglobulin ◽  
Antibody Titers ◽  
The Brain

Subacute sclerosing panencephalitis (SSPE) is a slowly progressing disease of the CNS in children which is caused by measles virus. Ferrets immunized with measles virus prior to inoculation with the cell associated, syncytiogenic D.R. strain of SSPE virus exhibit characteristics very similar to the human disease. Measles virus nucleocapsids are present, high measles antibody titers are found in the sera and inflammatory lesions are prominent in the brains. Measles virus specific immunoglobulin G (IgG) is present in the brain,and IgG/ albumin ratios indicate that the antibodies are synthesized within the CNS.

Therapeutic drug monitoring (TDM) of the recent antipsychotic ziprasidone in dried blood spots (DBS) and plasma

2011 ◽  
Vol 44 (06) ◽  
Author(s):  
L Mercolini ◽  
G Fulgenzi ◽  
M Melis ◽  
G Boncompagni ◽  
LJ Albers ◽  
...  
Keyword(s):  
Therapeutic Drug Monitoring ◽  
Drug Monitoring ◽  
Dried Blood Spots ◽  
Therapeutic Drug ◽  
Blood Spots
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