Variability in the precore and core promoter region of the hepatitis B virus genome

2013 ◽  
Vol 86 (3) ◽  
pp. 437-445 ◽  
Author(s):  
Maria Nordin ◽  
Mikael Ingman ◽  
Beata Lindqvist ◽  
Karin Kidd-Ljunggren
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Promoter Region ◽  
Core Promoter ◽  
Virus Genome ◽  
Core Promoter Region ◽  
B Virus

scholarly journals High Frequency of Either Altered Pre-Core Start Codon or Weakened Kozak Sequence in the Core Promoter Region in Hepatitis B Virus A1 Strains from Rwanda

Genes ◽  
2019 ◽  
Vol 10 (3) ◽  
pp. 182 ◽  
Author(s):  
Heléne Norder ◽  
Theogene Twagirumugabe ◽  
Joanna Said ◽  
Yarong Tian ◽  
Ka-Wei Tang ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Promoter Region ◽  
Core Promoter ◽  
Etiologic Agent ◽  
Start Codon ◽  
Core Promoter Region ◽  
Kozak Sequence ◽  
The Core ◽  
B Virus

Hepatitis B virus (HBV) is endemic in Rwanda and is a major etiologic agent for chronic liver disease in the country. In a previous analysis of HBV strains from Rwanda, the S genes of most strains segregated into one single clade of subgenotype, A1. More than half (55%) of the anti-HBe positive individuals were viremic. In this study, 23 complete HBV genomes and the core promoter region (CP) from 18 additional strains were sequenced. Phylogenetic analysis of complete genomes confirmed that most Rwandan strain formed a single unique clade, within subgenotype A1. Strains from 17 of 22 (77%) anti-HBe positive HBV carriers had either mutated the precore start codon (9 strains with either CUG, ACG, UUG, or AAG) or mutations in the Kozak sequence preceding the pre-core start codon (8 strains). These mutually exclusive mutations were also identified in subgenotypes A1 (70/266; 26%), A2 (12/255; 5%), and A3 (26/49; 53%) sequences from the GenBank. The results showed that previous, rarely described HBV variants, expressing little or no HBeAg, are selected in anti-HBe positive subgenotype Al carriers from Rwanda and that mutations reducing HBeAg synthesis might be unique for a particular HBV clade, not just for a specific genotype or subgenotype.

Mutations in the core promoter region of hepatitis B virus in patients with chronic hepatitis B

1996 ◽  
Vol 49 (2) ◽  
pp. 115-123 ◽  
Author(s):  
Masayuki Kurosaki ◽  
Nobuyuki Enomoto ◽  
Yasuhiro Asahina ◽  
Ikuo Sakuma ◽  
Takaaki Ikeda ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Chronic Hepatitis B ◽  
Promoter Region ◽  
Core Promoter ◽  
Core Promoter Region ◽  
The Core ◽  
B Virus

Naturally occurring hepatitis B virus mutants with deletions in the core promoter region

1994 ◽  
Vol 20 (6) ◽  
pp. 837-841 ◽  
Author(s):  
Tomasz Laskus ◽  
Jorge Rakela ◽  
Myron J. Tong ◽  
Marek J. Nowicki ◽  
James W. Mosley ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Promoter Region ◽  
Core Promoter ◽  
Core Promoter Region ◽  
The Core ◽  
Naturally Occurring ◽  
B Virus

scholarly journals Identification and characterization of a G-quadruplex structure in the pre-core promoter region of hepatitis B virus

2021 ◽  
Author(s):  
Vanessa Meier-Stephenson ◽  
Maulik D Badmalia ◽  
Tyler Mrozowich ◽  
Keith CK Lau ◽  
Sarah K Schultz ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Promoter Region ◽  
Core Promoter ◽  
Host Protein ◽  
Core Promoter Region ◽  
Hbv Cccdna ◽  
Increased Risk ◽  
B Virus ◽  
G Quadruplex

ABSTRACTWorldwide, ∼250 million people are chronically infected with the hepatitis B virus (HBV) and are at increased risk of cirrhosis and hepatocellular carcinoma. The HBV persists as covalently closed circular DNA (cccDNA), which acts as the template for all HBV mRNA transcripts. Nucleos(t)ide analogs do not directly target the HBV cccDNA and cannot eradicate the HBV. We have discovered a unique structural motif, a G-quadruplex in HBV’s pre-core promoter region that is conserved amongst nearly all genotypes, and is central to critical steps in the viral life-cycle including the production of pre-genomic RNA, core and polymerase proteins, and encapsidation. Thus, an increased understanding of the HBV pre-core may lead to the development of novel anti-HBV cccDNA targets. We utilized biophysical methods to characterize the presence of the G-quadruplex, employed assays using a known quadruplex-binding protein (DHX36) to pull-down HBV cccDNA, and compared HBV infection in HepG2 cells transfected with wild-type and mutant HBV plasmids. This study provides insights into the presence of a G-quadruplex in the HBV pre-core promoter region essential for HBV replication. The evaluation of this critical host-protein interaction site in the HBV cccDNA may ultimately facilitate the development of novel anti-HBV therapeutics against the resilient cccDNA template.

Genetic heterogeneity of the precore and the core promoter region of genotype C hepatitis B virus during lamivudine therapy

2003 ◽  
Vol 72 (1) ◽  
pp. 26-34 ◽  
Author(s):  
Reiichiro Kuwahara ◽  
Ryukichi Kumashiro ◽  
Shiro Murashima ◽  
Kei Ogata ◽  
Kazuo Tanaka ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Genetic Heterogeneity ◽  
Promoter Region ◽  
Core Promoter ◽  
Core Promoter Region ◽  
Lamivudine Therapy ◽  
The Core ◽  
B Virus ◽  
Genotype C
Sign in / Sign up

Export Citation Format

Share Document