Molecular characterization of norovirus variants and genetic diversity of noroviruses and sapoviruses in Thailand

2013 ◽  
Vol 86 (7) ◽  
pp. 1210-1218 ◽  
Author(s):  
Natthawan Chaimongkol ◽  
Pattara Khamrin ◽  
Rungnapa Malasao ◽  
Aksara Thongprachum ◽  
Tipachan Kongsricharoern ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Characterization

Molecular characterization of echovirus 13 uncovering high genetic diversity and identification of new genotypes in Pakistan

2017 ◽  
Vol 48 ◽  
pp. 102-108
Author(s):  
Shahzad Shaukat ◽  
Mehar Angez ◽  
Tariq Mahmood ◽  
Muhammad Masroor Alam ◽  
Salmaan Sharif ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Characterization ◽  
High Genetic Diversity

scholarly journals Molecular Characterization of Pathogenicity Locus (PaLoc) and tcdC Genetic Diversity Among tcdA+B+ Clostridioides Difficile Clinical Isolates in Tehran, Iran

2020 ◽  
Author(s):  
Mansoor Kodori ◽  
Zohreh Ghalavand ◽  
Abbas Yadegar ◽  
Gita Eslami ◽  
Masoumeh Azimirad ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Characterization ◽  
Disease Severity ◽  
Genetic Variants ◽  
Rflp Analysis ◽  
Clostridioides Difficile ◽  
Pcr Rflp ◽  
The Common ◽  
Healthcare Associated

Abstract Background: Clostridioides difficile is the main cause of healthcare-associated diarrhea worldwide. It is proposed that certain C. difficile toxinotypes with distinct pathogenicity locus (PaLoc) variants are associated with disease severity and outcomes. Additionally, few studies have described the common C. difficile toxinotypes, and also little is known about the tcdC variants in Iranian isolates. We characterized the toxinotypes and the tcdC genotypes from a collection of Iranian clinical C. difficile tcdA+B+ isolates with known ribotypes (RTs).Methods: Fifty C. difficile isolates with known RTs and carrying the tcdA and tcdB toxin genes were analyzed. Toxinotyping was carried out based on a PCR-RFLP analysis of a 19.6 kb region encompassing the PaLoc. Genetic diversity of the tcdC gene was determined by the sequencing of the gene.Results: Of the 50 C. difficile isolates investigated, five distinct toxinotypes were recognized. Toxinotypes 0 (33/50, 66%) and V (11/50, 22%) were the most frequently found. C. difficile isolates of the toxinotype 0 mostly belonged to RT 001 (12/33, 36.4%), whereas toxinotype V consisted of RT 126 (9/11, 81.8%). The tcdC sequencing showed six variants (35/50, 70%); tcdC-sc3 (24%), tcdC-A (22%), tcdC-sc9 (18%), tcdC-B (2%), tcdC-sc14 (2%), and tcdC-sc15 (2%). The remaining isolates were wild-types (15/50, 30%) in the tcdC gene.Conclusions: The present study demonstrates that the majority of clinical tcdA+B+ isolates of C. difficile frequently harbor tcdC genetic variants. We also found that the RT 001/ toxinotype 0 and the RT 126/ toxinotype V are the most common types among Iranian isolates. Further studies are needed to investigate the putative association of various tcdC genotypes with CDI severity and its recurrence.

Molecular characterization of genetic diversity and similarity centers of safflower accessions with ISSR markers

2020 ◽  
Vol 43 (1) ◽  
pp. 109-121 ◽  
Author(s):  
Fawad Ali ◽  
Muhammad Azhar Nadeem ◽  
Ephrem Habyarimana ◽  
Abdurrahim Yılmaz ◽  
Muhammad Amjad Nawaz ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Characterization ◽  
Issr Markers

Genetic diversity and molecular characterization of Babesia motasi -like in small ruminants and ixodid ticks from China

2016 ◽  
Vol 41 ◽  
pp. 8-15 ◽  
Author(s):  
Qingli Niu ◽  
Zhijie Liu ◽  
Jifei Yang ◽  
Peifa Yu ◽  
Yuping Pan ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Characterization ◽  
Small Ruminants ◽  
Ixodid Ticks

scholarly journals Molecular Characterization of Integrons in Epidemiologically Unrelated Clinical Isolates of Acinetobacter baumannii from Italian Hospitals Reveals a Limited Diversity of Gene Cassette Arrays

2002 ◽  
Vol 46 (11) ◽  
pp. 3665-3668 ◽  
Author(s):  
Francesca Gombac ◽  
Maria Letizia Riccio ◽  
Gian Maria Rossolini ◽  
Cristina Lagatolla ◽  
Enrico Tonin ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Acinetobacter Baumannii ◽  
Molecular Characterization ◽  
Horizontal Transfer ◽  
Gene Cassette ◽  
Clinical Isolates

ABSTRACT Integron carriage by 36 epidemiologically unrelated Acinetobacter baumannii isolates collected over an 11-year period from patients in six different Italian hospitals was investigated. Sixteen type 1 integron-positive isolates (44%) were found, 13 of which carried the same array of cassettes, i.e., aacC1, orfX, orfX′, and aadA1a. As ribotype analysis of the isolates demonstrated a notable genetic diversity, horizontal transfer of the entire integron structure or ancient acquisition was hypothesized.

Molecular characterization of pathogenicity locus (PaLoc) and tcdC genetic diversity among tcdAB Clostridioides difficile clinical isolates in Tehran, Iran

Anaerobe ◽  
2020 ◽  
Vol 66 ◽  
pp. 102294
Author(s):  
Mansoor Kodori ◽  
Zohreh Ghalavand ◽  
Abbas Yadegar ◽  
Gita Eslami ◽  
Masoumeh Azimirad ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Characterization ◽  
Clinical Isolates ◽  
Clostridioides Difficile

scholarly journals Molecular characterization of a farmer-preferred maize landrace population from a multiple-stress-prone subtropical lowland environment

2021 ◽  
Vol 22 (2) ◽  
Author(s):  
Fortunate Makore ◽  
Edmore Gasura ◽  
Caleb Souta ◽  
Upenyu Mazarura ◽  
John Derera ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Characterization ◽  
Allelic Variation ◽  
Polymorphic Information Content ◽  
Heterotic Group ◽  
Heterotic Groups ◽  
Multiple Stress ◽  
Maize Landrace ◽  
Landrace Population

Abstract. Makore F, Gasura E, Souta C, Mazamura U, Derera J, Zikhali M, Kamutando CN, Magorokosho C, Dari S. 2021. Molecular characterization of a farmer-preferred maize landrace population from a multiple-stress-prone subtropical lowland environment. Biodiversitas 22: 769-777.  The study was conducted to assess genetic diversity of 372 maize lines using 116 single nucleotide polymorphism (SNP) markers. Three hundred and forty-seven lines were S1 lines (coded J lines) from a local maize landrace population and twenty-five were the widely used standard lines. The number of alleles per marker ranged from two to four and the average was three alleles.  The average polymorphic information content (PIC) value of 0.405 indicates high genetic diversity for maize lines evaluated in this study. Population structure revealed three distinct sub-populations. Sub-population 1 contained two J lines; sub-population 2 contained five J lines and sub-population 3 contained the rest of the J lines and all the standard lines. Analysis of molecular variance (AMOVA) identified 22% variance among and 78% variance within the three subpopulations, indicating high gene exchange and low genetic differentiation. Hierarchical cluster analysis further divided the lines into nine subgroups placing some of the J lines into known heterotic groups', i.e.,  J30_3, J393_4, J393_3, and J393_1 in CIMMYT heterotic group B. Allelic variation observed can be a source of allele combination for breeding programs interested in widening their genetic base. The private alleles that were present in the J lines suggest availability of stress-tolerant genes that breeders can incorporate in new hybrids.

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