Characterization of the NSP4 gene of group A human rotavirus G1P[8] strains circulating in Sapporo, Japan from 1987 to 2000

2013 ◽  
Vol 86 (2) ◽  
pp. 354-359 ◽  
Author(s):  
Masatoshi Tatsumi ◽  
Yoshinobu Nagaoka ◽  
Takeshi Tsugawa ◽  
Yuko Yoto ◽  
Tsukasa Hori ◽  
...  
Keyword(s):  
Nsp4 Gene ◽  
Human Rotavirus ◽  
Group A

scholarly journals Molecular characterization of group A rotaviruses detected in children with gastroenteritis in Ireland in 2006–2009

2011 ◽  
Vol 140 (2) ◽  
pp. 247-259 ◽  
Author(s):  
O. CASHMAN ◽  
P. J. COLLINS ◽  
G. LENNON ◽  
B. CRYAN ◽  
V. MARTELLA ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Irish Population ◽  
Surveillance Activity ◽  
Human Rotavirus ◽  
Group A Rotaviruses ◽  
Group A ◽  
Hospital Acquired ◽  
Rotavirus Infections ◽  
Pcr Genotyping

SUMMARYCommunity and hospital-acquired cases of human rotavirus are responsible for millions of gastroenteritis cases in children worldwide, chiefly in developing countries, and vaccines are now available. During surveillance activity for human rotavirus infections in Ireland, between 2006 and 2009, a total of 420 rotavirus strains were collected and analysed. Upon either PCR genotyping and sequence analysis, a variety of VP7 (G1–G4 and G9) and VP4 (P[4], P[6], P[8] and P[9]) genotypes were detected. Strains G1P[8] were found to be predominant throughout the period 2006–2008, with slight fluctuations seen in the very limited samples available in 2008–2009. Upon either PCR genotyping and sequence analysis of selected strains, the G1, G3 and G9 viruses were found to contain E1 (Wa-like) NSP4 and I1 VP6 genotypes, while the analysed G2 strains possessed E2 NSP4 and I2 VP6 genotypes, a genetic make-up which is highly conserved in the major human rotavirus genogroups Wa- and Kun-like, respectively. Upon sequence analysis of the most common VP4 genotype, P[8], at least two distinct lineages were identified, both unrelated to P[8] Irish rotaviruses circulating in previous years, and more closely related to recent European humans rotaviruses. Moreover, sequence analysis of the VP7 of G1 rotaviruses revealed the onset of a G1 variant, previously unseen in the Irish population.

scholarly journals Molecular characterisation of the NSP4 gene of group A human rotavirus G2P[4] strains circulating in São Paulo, Brazil, from 1994 and 2006 to 2010

2015 ◽  
Vol 110 (6) ◽  
pp. 786-792 ◽  
Author(s):  
Jéssica Wildgrube Bertol ◽  
Maria Clara Duarte Fregolente ◽  
Thabata Alessandra Ramos Caruzo ◽  
Márcio José da Silva ◽  
Veridiana Munford ◽  
...  
Keyword(s):  
Sao Paulo ◽  
Molecular Characterisation ◽  
São Paulo ◽  
Nsp4 Gene ◽  
Human Rotavirus ◽  
Group A

Molecular characterization of VP6-encoding gene of group A human rotavirus samples from central west region of Brazil

2008 ◽  
Vol 80 (11) ◽  
pp. 2034-2039 ◽  
Author(s):  
Talissa de Moraes Tavares ◽  
Wilia Marta Elsner Diederichsen de Brito ◽  
Fabíola Souza Fiaccadori ◽  
Juliana Alves Parente ◽  
Paulo Sérgio Sucasas da Costa ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
West Region ◽  
Human Rotavirus ◽  
Group A ◽  
Encoding Gene

scholarly journals Molecular characterization of the NSP4 gene of human group A rotavirus samples from the West Central region of Brazil

2008 ◽  
Vol 103 (3) ◽  
pp. 288-294 ◽  
Author(s):  
Talissa de Moraes Tavares ◽  
Wilia Marta Elsner Diederichsen de Brito ◽  
Fabíola Souza Fiaccadori ◽  
Erika Regina Leal de Freitas ◽  
Juliana Alves Parente ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Central Region ◽  
Nsp4 Gene ◽  
Human Group ◽  
The West ◽  
Group A Rotavirus ◽  
West Central ◽  
Group A

scholarly journals Assignment of the group A rotavirus NSP4 gene into genotypes using a hemi-nested multiplex PCR assay: a rapid and reproducible assay for strain surveillance studies

2009 ◽  
Vol 58 (3) ◽  
pp. 303-311 ◽  
Author(s):  
Krisztián Bányai ◽  
Ágnes Bogdán ◽  
György Szücs ◽  
Serenella Arista ◽  
Simona De Grazia ◽  
...  
Keyword(s):  
Vaccine Development ◽  
Interspecies Transmission ◽  
Reference Laboratory ◽  
Nsp4 Gene ◽  
Structural Protein ◽  
Multiplex Pcr Assay ◽  
Human Rotavirus ◽  
Group A ◽  
P Type ◽  
Animal Strains

The rotavirus non-structural protein NSP4 has been implicated in a number of biological functions during the rotavirus cellular cycle and pathogenesis, and has been addressed as a target for vaccine development. The NSP4 gene has been classified into six genotypes (A–F). A semi-nested triplex PCR was developed for genotyping the major human NSP4 genotypes (A–C), which are common in human rotavirus strains but are also shared among most mammalian rotavirus strains. A total of 192 previously characterized human strains representing numerous G and P type specificities (such as G1P[8], G1P[4], G2P[4], G3P[3], G3P[8], G3P[9], G4P[6], G4P[8], G6P[4], G6P[9], G6P[14], G8P[10], G8P[14], G9P[8], G9P[11], G10P[11], G12P[6] and G12P[8]) were tested for NSP4 specificity by the collaborating laboratories. An additional 35 animal strains, including the reference laboratory strains SA11 (simian, G3P[2]), NCDV (bovine, G6P[1]), K9 and CU-1 (canine, G3P[3]), together with 31 field isolates (canine, G3P[3]; feline, G3P[9]; porcine, G2P[23], G3P[6], G4P[6], G5P[6], G5P[7], G5P[26], G5P[27], G9P[6] and G9P[7]) were also successfully NSP4-typed. Four human G3P[9] strains and one feline G3P[9] strain were found to possess an NSP4 A genotype, instead of NSP4 C, suggesting a reassortment event between heterologous strains. Routine NSP4 genotyping may help to determine the genomic constellation of rotaviruses of man and livestock, and identify interspecies transmission of heterologous strains.

Molecular characterization of the NSP4 gene of human group A rotavirus strains circulating in Tunisia from 2006 to 2008

2012 ◽  
Vol 12 (5) ◽  
pp. 997-1004 ◽  
Author(s):  
Mouna Ben Hadj Fredj ◽  
Mark Zeller ◽  
Imene Fodha ◽  
Elisabeth Heylen ◽  
Anissa Chouikha ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Nsp4 Gene ◽  
Human Group ◽  
Group A Rotavirus ◽  
Group A

scholarly journals Molecular characterization of a rare G1P[19] rotavirus strain from India: evidence of reassortment between human and porcine rotavirus strains

2009 ◽  
Vol 58 (12) ◽  
pp. 1611-1615 ◽  
Author(s):  
Shobha D. Chitambar ◽  
Ritu Arora ◽  
Preeti Chhabra
Keyword(s):  
Amino Acid Sequences ◽  
Rt Pcr ◽  
Coding Regions ◽  
Subgroup Ii ◽  
Human Rotavirus ◽  
Independent Segregation ◽  
Group A ◽  
Porcine Rotavirus ◽  
Porcine Origin

This study pertains to the characterization of a human rotavirus strain (NIV929893) with a rare specificity of G1P[19]. Three structural genes (VP4, VP6 and VP7) and one non-structural gene (NSP4) of strain NIV929893 were subjected to RT-PCR for amplification of entire coding regions. All of the amplicons were sequenced to carry out phylogenetic analysis. The complete amino acid sequences of the VP7 and VP4 gene products showed clustering of the VP7 gene with G1 strains of human origin and the VP4 gene with P[19] strains of porcine origin. The two viral proteins VP6 and NSP4, described previously as genetically linked proteins, were shown to be subgroup II and genotype B of human and porcine origins, respectively. The findings of this study provide evidence of reassortment between VP7/VP6 genes of humans and VP4/NSP4 genes of porcine species and an independent segregation of VP6 and NSP4 genes in a group A human rotavirus strain with G1P[19] specificity.

scholarly journals Molecular Characterization of VP6 Genes of Human Rotavirus Isolates: Correlation of Genogroups with Subgroups and Evidence of Independent Segregation

2002 ◽  
Vol 76 (13) ◽  
pp. 6596-6601 ◽  
Author(s):  
Miren Iturriza Gómara ◽  
Cecilia Wong ◽  
Sandra Blome ◽  
Ulrich Desselberger ◽  
Jim Gray
Keyword(s):  
Molecular Characterization ◽  
Rt Pcr ◽  
Genetic Lineages ◽  
Reverse Transcription Pcr ◽  
Human Rotavirus ◽  
Independent Segregation ◽  
Genes Encoding ◽  
Group A Rotaviruses ◽  
Group A

ABSTRACT A reverse transcription-PCR (RT-PCR) was established to amplify a 379-bp cDNA fragment (nucleotides 747 to 1126, coding for amino acids 241 to 367) of the VP6 gene of group A rotaviruses associated with subgroup (SG) specificity. Thirty-eight human rotavirus strains characterized with SG-specific monoclonal antibodies were subjected to VP6-specific RT-PCR, and PCR amplicons were used for sequencing. Nucleic acid sequencing and phylogenetic analysis of the VP6 amplicons revealed two clusters, or genogroups. Two genetic lineages were distinguished within genogroup I, consisting of strains serologically characterized as SG I, and three genetic lineages were distinguished within genogroup II, composed of strains serologically characterized as SG II, SG I + II, and SG non-I, non-II. Subgrouping of rotaviruses by means of serological methods may result in strains not being assigned the correct SG or in a failure of strains to subgroup. Molecular characterization of the SG-defining region of VP6 provided evidence for independent segregation of the rotavirus genes encoding VP4, VP6, and VP7.

scholarly journals Molecular Characterization of Human Rotavirus from Children with Diarrhoeal Disease in Sokoto State, Nigeria

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
B. R. Alkali ◽  
A. I. Daneji ◽  
A. A. Magaji ◽  
L. S. Bilbis ◽  
F. Bande
Keyword(s):  
Monoclonal Antibody ◽  
Diarrhoeal Disease ◽  
Molecular Study ◽  
Rt Pcr ◽  
Human Rotavirus ◽  
Group A ◽  
The Common ◽  
Polymerase Chain ◽  
Pcr Techniques

This study was conducted to detect and characterize prevalent human group A rotavirus strains from 200 diarrheic children in Sokoto, Nigeria, by ELISA, monoclonal antibody (Mab) serotyping and Reverse Transcription-Polymerase Chain Reaction (RT-PCR) techniques. Rotavirus was detected in 25.5% of the children. The G-serotypes observed in circulation were G4: 16 (59.3%), G1: 4 (14.8%), G2: 3 (11.1%), G3: 3 (11.1%), and G12: 1 (3.7%). The monoclonal antibody (Mab) serotyping detected G1 and G3 but did not detect G4 and G2 serotypes. The Mab typing of the G1 and G3 serotypes was consistent with the result of the RT-PCR. The VP4 genotypes detected were P[6] 3 (13%), P[8] 11 (47.8%), and the rare human P genotype (P[9]), found in 9 patients (39.1%). Nine strains identified with the common G and P combinations were G4 P[8] 5 (56%), G4 P[6] 1 (11%), G1 P[8] 2 (22%), and G3 P[8] 1 (11%), while seven strains with unusual combinations or rare G or P genotypes identified were G12 P[8] 1 (14%), G2 P[8] 2 (29%), and G4 P[9] 4 (57%). To our knowledge this is the first molecular study of human rotavirus and report of rare human G and P serotypes in Sokoto State.

Genotyping and sequence characterization of the NSP4 gene of human group A rotavirus strains in Northern Iran

2021 ◽  
Author(s):  
Parinaz Khalkhali ◽  
Armin Khavandegar ◽  
Sayed‐Hamidreza Mozhgani ◽  
Ali Teimoori ◽  
Abdolvahhab Moradi ◽  
...  
Keyword(s):  
Nsp4 Gene ◽  
Human Group ◽  
Northern Iran ◽  
Sequence Characterization ◽  
Group A Rotavirus ◽  
Group A
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