Human papillomavirus was not detected by PCR using multiple consensus primer sets in esophageal adenocarcinomas in Chinese patients

2013 ◽  
Vol 85 (6) ◽  
pp. 1053-1057 ◽  
Author(s):  
Songtao Feng ◽  
Jianming Zheng ◽  
Xiang Du ◽  
Yunshan Tan ◽  
Haijun Yang ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Chinese Patients ◽  
Consensus Primer ◽  
Primer Sets

scholarly journals Detection of human papillomavirus in cervical cell specimens by hybrid capture and PCR with different primers.

2006 ◽  
Vol 53 (3) ◽  
pp. 603-607 ◽  
Author(s):  
Slawa Szostek ◽  
Malgorzata Klimek ◽  
Barbara Zawilinska ◽  
Janusz Rys ◽  
Jolanta Kope ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Consensus Primer ◽  
Hybrid Capture ◽  
Hpv Dna ◽  
Cervical Smears ◽  
Capture Assay ◽  
Cervical Cell ◽  
Target Dna ◽  
Primer Sets

The purpose of this study was to compare hybrid capture assay with PCRs using different primers for the L1, E6-E7 regions for the detection of human papillomavirus (HPV) genome. One hundred twenty-five cervical smears with normal (n=42) and abnormal (n=83) cytology were investigated. Those at high-risk for HPV were studied by hybridization antibody capture assay and PCR with the pU-1M/pU-2R primers. Target DNA from the HPV L1 region was amplified by SPF10 primer set and home-PCR with MY09/MY11 primers. The presence of HPV DNA in cervical smears was detected by SPF10 (in 72% of cases), MY09/MY11 (58%), hybrid capture (55%) and pU-1M/pU-2R (39%). Results obtained with the SPF10 and MY09/MY11 consensus primer sets as well as hybrid capture and pU-1M/pU-2R specific for high-risk types differed significantly (chi2, P

Human Papillomavirus Genome based Detection and Typing: A Holistic Molecular Approach

2019 ◽  
Vol 19 (4) ◽  
pp. 237-246 ◽  
Author(s):  
Abhilasha Gautam ◽  
Mallikarjuna R. Gedda ◽  
Madhukar Rai ◽  
Shyam Sundar ◽  
Jaya Chakravarty
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Genome Structure ◽  
Cost Effective ◽  
Detection Sensitivity ◽  
Screening Methods ◽  
Consensus Primer ◽  
Hpv Dna ◽  
Primer Sets ◽  
Consensus Primers

Human Papillomavirus (HPV) is a species specific double-stranded DNA virus infecting human cutaneous or mucosal tissues. The genome structure of HPV is extremely polymorphic hence making it difficult to discriminate between them. HPV exhibits numerous dissimilar types that can be subdivided into high-risk (HR), probably high-risk and low-risk (LR), causing numerous types of cancers and warts around the genital organs in humans. Several screening methods are performed in order to detect cytological abnormalities and presence or absence of HPV genome. Currently available commercial kits and methods are designed to detect only a few HR/LR-HPV types, which are expensive adding to the economic burden of the affected individual and are not freely available. These gaps could be minimized through Polymerase Chain reaction (PCR) method, which is a gold standard and a cost-effective technique for the detection of most HPV (both known and unknown) types by using specific consensus primers in minimal lab setup. In this context, numerous studies have validated the effectiveness of different sets of consensus primers in the screening of HPVs. Numerous consensus primers, such as E6, E6/E7, GP-E6/E7, MY09/11, GP5+/GP6+, SPF10, and PGMY09/11 have been developed to detect the presence of HPV DNA. In addition, HPV detection sensitivity could be achieved through consensus primer sets targeting specific ORF regions like L1 and E6, which may finally assist in better diagnosis of several unknown HR-HPVs. The present review, provides a summary of the available methods, kits and consensus primer sets for HPV genome based detection, their advantages and limitations along with future goals to be set for HPV detection.

The Prevalence of Human Papillomavirus Type 58 in Chinese Patients with Cervical Carcinoma and its Influence on Survival

2009 ◽  
Vol 21 (10) ◽  
pp. 768-774 ◽  
Author(s):  
Walayat Shah ◽  
Chen Hongwei ◽  
Zheng Jin ◽  
Dong Lifang ◽  
Yu Jun ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Cervical Carcinoma ◽  
Chinese Patients

scholarly journals Genotyping of 27 Human Papillomavirus Types by Using L1 Consensus PCR Products by a Single-Hybridization, Reverse Line Blot Detection Method

1998 ◽  
Vol 36 (10) ◽  
pp. 3020-3027 ◽  
Author(s):  
P. E. Gravitt ◽  
C. L. Peyton ◽  
R. J. Apple ◽  
C. M. Wheeler
Keyword(s):  
Human Papillomavirus ◽  
Detection System ◽  
Sampling Error ◽  
Blot Hybridization ◽  
High Sensitivity ◽  
Oligonucleotide Probes ◽  
Dot Blot ◽  
Consensus Primer ◽  
Hpv Dna ◽  
Silver Spring

Amplification of human papillomavirus (HPV) DNA by L1 consensus primer systems (e.g., MY09/11 or GP5+/6+) can detect as few as 10 to 100 molecules of HPV targets from a genital sample. However, genotype determination by dot blot hybridization is laborious and requires at least 27 separate hybridizations for substantive HPV-type discrimination. A reverse blot method was developed which employs a biotin-labeled PCR product hybridized to an array of immobilized oligonucleotide probes. By the reverse blot strip analysis, genotype discrimination of multiple HPV types can be accomplished in a single hybridization and wash cycle. Twenty-seven HPV probe mixes, two control probe concentrations, and a single reference line were immobilized to 75- by 6-mm nylon strips. Each individual probe line contained a mixture of two bovine serum albumin-conjugated oligonucleotide probes specific to a unique HPV genotype. The genotype spectrum discriminated on this strip includes the high-risk, or cancer-associated, HPV genotypes 16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 55, 56, 58, 59, 68 (ME180), MM4 (W13B), MM7 (P291), and MM9 (P238A) and the low-risk, or non-cancer-associated, genotypes 6, 11, 40, 42, 53, 54, 57, 66, and MM8 (P155). In addition, two concentrations of β-globin probes allowed for assessment of individual specimen adequacy following amplification. We have evaluated the performance of the strip method relative to that of a previously reported dot blot format (H. M. Bauer et al., p. 132–152, in C. S. Herrington and J. O. D. McGee (ed.), Diagnostic Molecular Pathology: a Practical Approach, (1992), by testing 328 cervical swab samples collected in Digene specimen transport medium (Digene Diagnostics, Silver Spring, Md.). We show excellent agreement between the two detection formats, with 92% concordance for HPV positivity (kappa = 0.78, P < 0.001). Nearly all of the discrepant HPV-positive samples resulted from weak signals and can be attributed to sampling error from specimens with low concentrations (<1 copy/μl) of HPV DNA. The primary advantage of the strip-based detection system is the ability to rapidly genotype HPVs present in genital samples with high sensitivity and specificity, minimizing the likelihood of misclassification.

scholarly journals Comparison of PCR- and Hybrid Capture-Based Human Papillomavirus Detection Systems Using Multiple Cervical Specimen Collection Strategies

1998 ◽  
Vol 36 (11) ◽  
pp. 3248-3254 ◽  
Author(s):  
C. L. Peyton ◽  
M. Schiffman ◽  
A. T. Lörincz ◽  
W. C. Hunt ◽  
I. Mielzynska ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Consensus Primer ◽  
Hybrid Capture ◽  
Detection Systems ◽  
Specimen Collection ◽  
Alternative Procedures ◽  
Cervical Specimen ◽  
Cytology Brush ◽  
Lower Cutoff ◽  
Specimen Transport Medium

This study compared the performances of three human papillomavirus (HPV) detection tests with specimens collected by three alternative procedures. The HPV tests included the Hybrid Capture Tube test (HCT), the microplate-based Hybrid Capture II test (HC II), and the MY09-MY11 L1 consensus primer PCR-based assay. Initial cervical specimens were collected from study subjects with a broom device, and after Papanicolaou smears were made, residual specimens were placed into PreservCyt (PC), a liquid cytology medium. A second specimen was collected from each subject and placed into Digene Specimen Transport Medium (STM). The device for collection of the second specimen alternated with consecutive subjects between a conical cytology brush and a Dacron swab. At the 1.0-pg/ml cutoff, the results of the HC II agreed well with those of the PCR. Specifically, when PCR data were restricted to the types found by the HC II (HPV types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, and 68), there was greater than 90% agreement between the HC II and PCR results with both STM and PC. At a lower cutoff (0.2 pg/ml), HC II-positive results increased further, especially when the test was applied to the PC specimens. However, false-positive HC II results were more often observed at the 0.2-pg/ml cutoff. HC II yielded the highest HPV positivity with specimens placed into PC, followed by specimens collected with a conical brush and placed into STM and, last, by those collected with a Dacron swab and placed into STM. Our results demonstrate the utility of both the STM and PC specimen collection methods and show good agreement between the HC II and PCR.

scholarly journals Low Prevalence of Human Papillomavirus (HPV) in Chinese Patients with Breast Cancer

2011 ◽  
Vol 39 (5) ◽  
pp. 1636-1644 ◽  
Author(s):  
X Mou ◽  
L Chen ◽  
F Liu ◽  
Y Shen ◽  
H Wang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Human Papillomavirus ◽  
Chinese Patients ◽  
Low Prevalence

Detection by PCR of human papillomavirus in Colombia: Comparison of GP5+/6+ and MY09/11 primer sets

2011 ◽  
Vol 178 (1-2) ◽  
pp. 68-74 ◽  
Author(s):  
Milena Camargo ◽  
Sara Soto-De Leon ◽  
Ricardo Sanchez ◽  
Marina Munoz ◽  
Erika Vega ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Primer Sets

Prevalence of human papillomavirus in Chinese patients with colorectal cancer

2010 ◽  
Vol 13 (8) ◽  
pp. 865-871 ◽  
Author(s):  
F. Liu ◽  
X. Mou ◽  
N. Zhao ◽  
J. Lin ◽  
L. Teng ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Human Papillomavirus ◽  
Chinese Patients
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