Frequency and clinical significance of core promoter and precore region mutations in Tunisian patients infected chronically with hepatitis B

2012 ◽  
Vol 84 (11) ◽  
pp. 1719-1726 ◽  
Author(s):  
Rim Ouneissa ◽  
Olfa Bahri ◽  
Nissaf Ben Alaya-Bouafif ◽  
Sofiene Chouaieb ◽  
Ahlem Ben Yahia ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Clinical Significance ◽  
Core Promoter ◽  
Precore Region ◽  
Tunisian Patients

Clinical significance of precore and core promoter mutations in genotype D hepatitis B-related chronic liver disease

2008 ◽  
Vol 15 (10) ◽  
pp. 753-760 ◽  
Author(s):  
H. Poustchi ◽  
A. Mohamadkhani ◽  
S. Bowden ◽  
G. Montazeri ◽  
A. Ayres ◽  
...  
Keyword(s):  
Liver Disease ◽  
Hepatitis B ◽  
Chronic Liver Disease ◽  
Clinical Significance ◽  
Core Promoter ◽  
Chronic Liver ◽  
Promoter Mutations

493 CLINICAL SIGNIFICANCE OF PRECORE AND CORE PROMOTER MUTATIONS IN KOREAN CHRONIC HEPATITIS B PATIENTS

2012 ◽  
Vol 56 ◽  
pp. S194
Author(s):  
S.Y. Yim ◽  
S.H. Um ◽  
J.D. Kim ◽  
J.Y. Jung ◽  
C.H. Kim ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis B ◽  
Chronic Hepatitis B ◽  
Clinical Significance ◽  
Core Promoter ◽  
Promoter Mutations

Association of hepatitis B virus subgenotypes and basal core promoter/precore region variants with the clinical features of patients with acute hepatitis

2008 ◽  
Vol 43 (7) ◽  
pp. 558-564 ◽  
Author(s):  
Kazuhiko Hayashi ◽  
Yoshiaki Katano ◽  
Yasushi Takeda ◽  
Takashi Honda ◽  
Masatoshi Ishigami ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Acute Hepatitis ◽  
Clinical Features ◽  
Core Promoter ◽  
Basal Core Promoter ◽  
Precore Region ◽  
B Virus

scholarly journals Molecular Epidemiology of an Outbreak of Fulminant Hepatitis B

2000 ◽  
Vol 38 (8) ◽  
pp. 2975-2981 ◽  
Author(s):  
Nicola Petrosillo ◽  
Giuseppe Ippolito ◽  
Laura Solforosi ◽  
Pietro E. Varaldo ◽  
Massimo Clementi ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Fulminant Hepatitis ◽  
Core Promoter ◽  
Pcr Amplification ◽  
Serum Samples ◽  
Nucleotide Substitutions ◽  
Source Of Infection ◽  
Precore Region ◽  
Route Of Transmission ◽  
Hbsag Positive Patient

A nosocomial outbreak of hepatitis B occurred among the inpatients of a hematology unit. Nine of the 11 infected patients died from fulminant hepatitis. An investigation was conducted to identify the source of infection and the route of transmission. Two clusters of nosocomial hepatitis B were identified. The hepatitis B virus (HBV) genome from serum samples of all case patients, of one HBsAg-positive patient with acute reactivation of the infection, and of eight acutely infected, unrelated cases was identified by PCR amplification of viral DNA and was entirely sequenced. Transmission was probably associated with breaks in infection control practices, which occurred as single events from common sources or through a patient-to-patient route, likely the result of shared medications or supplies. Sequence analysis evidenced close homology among the strains from the case patients and that from the patient with reactivation, who was the likely source of infection. Molecular analysis of viral isolates evidenced an accumulation of mutations in the core promoter/precore region, as well as several nucleotide substitutions throughout the genome. The sequences of all patients were compared with published sequences from fulminant and nonfulminant HBV infections.

Low frequency of mutations in the X gene, core promoter and precore region of hepatitis B virus infected Vietnamese

2005 ◽  
Vol 12 (2) ◽  
pp. 160-167 ◽  
Author(s):  
L. H. Song ◽  
D. N. Duy ◽  
V. Q. Binh ◽  
A. J. F. Luty ◽  
P. G. Kremsner ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Core Promoter ◽  
Low Frequency ◽  
Precore Region ◽  
B Virus ◽  
X Gene

Molecular characterization of Hepatitis B virus basal core promoter and precore region of isolates from chronic Hepatitis B patients

2021 ◽  
pp. 1-18
Author(s):  
Israr Ahmad ◽  
Kafeel Ahmad
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis B ◽  
Phylogenetic Tree ◽  
Chronic Hepatitis B ◽  
Stop Codon ◽  
Core Promoter ◽  
Direct Sequencing ◽  
Basal Core Promoter ◽  
Double Mutation ◽  
Precore Region

Abstract Objective: The aim of this study was to analyze mutations in precore/core promoter region of HBV genome in chronic hepatitis B patients from three cities of Pakistan. Methods: A total of 50 treatment naïve chronic hepatitis B patients from Pakistan were selected. Viral load, HBeAg/antiHBe status, HBV ELISA and ALT levels were determined. Direct sequencing of BCP and PC region of HBV genome was carried out following a nested PCR approach. Phylogenetic tree was constructed using MEGA software version 6.0.  Statistical analysis was carried out using SPSS version 16.0. Results: The G1896A precore stop codon mutation was detected in 19 (38%) isolates. The mutation was present in 17(34%) isolates from HBeAg negative patients and 2(4%) isolates from HBeAg positive patients. The Classic A1762T/G1764A double mutation was noted in 15 (30%) isolates. Mutation at position 1764 was observed in 12 (48%) samples. A rare G1764T mutation was also detected in 6 (12%) isolates. The CG1802-1803 mutation was detected in 47(94%) isolates. The T1858 mutation was detected in all 50 (100%) isolates. The GCAC Kozak sequence was present in 43(86%) isolates. The CAA1817-1819 mutation was observed in 49(98%) isolates and G1888 mutation was detected in 49(98%) isolates. Overall, 9(18%) isolates had wild-type sequences at all important loci including positions 1762,1764 and 1896. The pattern of sequences at genotype specific positions and phylogenetic tree revealed that majority of study isolates belonged to genotype D. Conclusions: Sequences results showed that precore region was comparatively more conserved than BCP region. Continuous...  

Sign in / Sign up

Export Citation Format

Share Document