Characterization of Epstein-Barr virus (EBV) BZLF1 gene promoter variants and comparison of cellular gene expression profiles in Japanese patients with infectious mononucleosis, chronic active EBV infection, and EBV-associated hemophagocytic lymphohistioc

2012 ◽  
Vol 84 (6) ◽  
pp. 940-946 ◽  
Author(s):  
Masayuki Imajoh ◽  
Yumiko Hashida ◽  
Masanao Murakami ◽  
Akihiko Maeda ◽  
Tetsuya Sato ◽  
...  
Keyword(s):  
Epstein Barr Virus ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Gene Promoter ◽  
Japanese Patients ◽  
Cellular Gene ◽  
Barr Virus ◽  
Bzlf1 Gene ◽  
Epstein Barr

scholarly journals Erratam: Epstein-Barr virus BZLF1 gene promoter variants in pediatric patients with acute infectious mononucleosis. Its comparison with pediatric lymphomas

2012 ◽  
Vol 84 (10) ◽  
pp. 1697-1697
Author(s):  
Mario Alejandro Lorenzetti ◽  
Marina Inés Gutiérrez ◽  
Jaime Altcheh ◽  
Guillermo Moscatelli ◽  
Samanta Moroni ◽  
...  
Keyword(s):  
Infectious Mononucleosis ◽  
Epstein Barr Virus ◽  
Pediatric Patients ◽  
Gene Promoter ◽  
Barr Virus ◽  
Bzlf1 Gene ◽  
Epstein Barr ◽  
Acute Infectious Mononucleosis

PCR array analysis of gene expression profiles in chronic active Epstein–Barr virus infection

2014 ◽  
Vol 16 (7) ◽  
pp. 581-586 ◽  
Author(s):  
Masanao Murakami ◽  
Yumiko Hashida ◽  
Masayuki Imajoh ◽  
Akihiko Maeda ◽  
Mikio Kamioka ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epstein Barr Virus ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Epstein Barr Virus Infection ◽  
Pcr Array ◽  
Array Analysis ◽  
Barr Virus ◽  
Epstein Barr ◽  
Barr Virus Infection

Epstein-Barr virus BZLF1 gene promoter variants in pediatric patients with acute infectious mononucleosis: Its comparison with pediatric lymphomas

2009 ◽  
Vol 81 (11) ◽  
pp. 1912-1917 ◽  
Author(s):  
Mario Alejandro Lorenzetti ◽  
Marina Inés Gutiérrez ◽  
Jaime Altcheh ◽  
Guillermo Moscatelli ◽  
Samanta Moroni ◽  
...  
Keyword(s):  
Infectious Mononucleosis ◽  
Epstein Barr Virus ◽  
Pediatric Patients ◽  
Gene Promoter ◽  
Barr Virus ◽  
Bzlf1 Gene ◽  
Epstein Barr ◽  
Acute Infectious Mononucleosis

scholarly journals Epstein-Barr virus promotes survival through germinal center light zone chromatin architecture

2020 ◽  
Author(s):  
Joanne Dai ◽  
Emma Heckenberg ◽  
Lingyun Song ◽  
Gregory E. Crawford ◽  
Micah A. Luftig
Keyword(s):  
B Cells ◽  
B Cell ◽  
Germinal Center ◽  
Epstein Barr Virus ◽  
Expression Profiles ◽  
Transcriptional Start Site ◽  
Gene Expression Profiles ◽  
Chromatin Accessibility ◽  
Barr Virus ◽  
Epstein Barr

ABSTRACTBFL-1 is an understudied anti-apoptotic protein upregulated in cancer and Epstein-Barr virus (EBV)-immortalized lymphoblastoid cell lines (LCLs). We have previously shown that BFL-1 is regulated through viral EBNA3A-mediated alterations in B-cell chromatin conformation (Price et al., 2017). Here, we extend those findings to define cis- and trans-acting factors that regulate BFL-1 in LCLs and reliance on BFL-1 for survival from extrinsic apoptosis. Beyond LCLs, BFL-1 is expressed in B cells maturing in the germinal center (GC). We therefore characterized the gene expression profiles and chromatin landscape of maturing human tonsillar B-cell subsets. While chromatin accessibility at the BFL-1 locus increases as naïve B cells enter the GC reaction, BFL-1 expression increases during the transition from dark zone to light zone (LZ) correlating with association between enhancer regions and the transcriptional start site. The relationship between LCLs and LZ B cells suggests that EBV phenocopies GC biology to enhance their survival in establishing latent infection.

scholarly journals Identification of a Negative cis Element within the ZII Domain of the Epstein-Barr Virus Lytic Switch BZLF1 Gene Promoter

1998 ◽  
Vol 72 (10) ◽  
pp. 8230-8239 ◽  
Author(s):  
Pingfan Liu ◽  
Shaofan Liu ◽  
Samuel H. Speck
Keyword(s):  
Epstein Barr Virus ◽  
Gene Promoter ◽  
Fold Increase ◽  
Lytic Cycle ◽  
Context Sensitive ◽  
Barr Virus ◽  
Cis Element ◽  
Latently Infected ◽  
Bzlf1 Gene ◽  
Epstein Barr

ABSTRACT The Epstein-Barr virus (EBV) lytic switch gene, BZLF1, is tightly regulated in latently infected B cells. The BZLF1 gene promoter (Zp) contains several cis elements that have been previously shown to respond to inducers of the viral lytic cycle. These include four copies of an element referred to as the ZI domains and an element that contains a consensus CRE/AP-1 motif (ZII domain). In addition, Zp is autoregulated through two sites that bind the BZLF1 gene product Zta. The ZI domains have been shown to bind the ubiquitous cellular transcription factors Sp1 and Sp3 and/or the myocyte enhancer factor 2D (Liu et al., EMBO J. 16:143–153, 1997; Liu et al., Virology 228:9–16, 1997). Here we present a functional analysis of the ZII domain and show: (i) ATF-1 and ATF-2 appear to be the predominant cellular factors that bind to the CRE/AP-1 motif present in the ZII domain; and (ii) the region immediately upstream of the CRE/AP-1 motif contains a potent negative cis element, mutation of which results in a >10-fold increase in Zp activity. The negative cis element (ZIIR) in the ZII domain decreases both basal and induced Zp activity and thus is likely to play an important role in regulating reactivation of EBV. In addition, analysis of heterologous promoter constructs indicates that the function of ZIIR is context sensitive. Attempts to demonstrate a cellular factor binding to ZIIR have been unsuccessful, leaving unresolved the mechanism by which repression is mediated.

Characterization of an Epstein-Barr virus-induced thymidine kinase.

1986 ◽  
Vol 57 (3) ◽  
pp. 1105-1112 ◽  
Author(s):  
M de Turenne-Tessier ◽  
T Ooka ◽  
G de The ◽  
J Daillie
Keyword(s):  
Thymidine Kinase ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Epstein Barr
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