scholarly journals Broad respiratory virus detection in infants hospitalized for bronchiolitis by use of a multiplex RT-PCR DNA microarray system

2012 ◽  
Vol 84 (6) ◽  
pp. 979-985 ◽  
Author(s):  
Antoine Huguenin ◽  
Lauryane Moutte ◽  
Fanny Renois ◽  
Nicolas Leveque ◽  
Deborah Talmud ◽  
...  
Keyword(s):  
Dna Microarray ◽  
Respiratory Virus ◽  
Virus Detection ◽  
Rt Pcr ◽  
Dna Microarray System

scholarly journals Comparison between Saliva and Nasopharyngeal Swab Specimens for Detection of Respiratory Viruses by Multiplex Reverse Transcription-PCR

2016 ◽  
Vol 55 (1) ◽  
pp. 226-233 ◽  
Author(s):  
Young-gon Kim ◽  
Seung Gyu Yun ◽  
Min Young Kim ◽  
Kwisung Park ◽  
Chi Hyun Cho ◽  
...  
Keyword(s):  
Real Time ◽  
Reverse Transcription ◽  
Respiratory Virus ◽  
Direct Sequencing ◽  
Virus Detection ◽  
Cost Effective ◽  
Respiratory Viruses ◽  
Nasopharyngeal Swab ◽  
Virus Species ◽  
Rt Pcr

ABSTRACT Nasopharyngeal swabs (NPSs) are being widely used as specimens for multiplex real-time reverse transcription (RT)-PCR for respiratory virus detection. However, it remains unclear whether NPS specimens are optimal for all viruses targeted by multiplex RT-PCR. In addition, the procedure to obtain NPS specimens causes coughing in most patients, which possibly increases the risk of nosocomial spread of viruses. In this study, paired NPS and saliva specimens were collected from 236 adult male patients with suspected acute respiratory illnesses. Specimens were tested for 16 respiratory viruses by multiplex real-time RT-PCR. Among the specimens collected from the 236 patients, at least 1 respiratory virus was detected in 183 NPS specimens (77.5%) and 180 saliva specimens (76.3%). The rates of detection of respiratory viruses were comparable for NPS and saliva specimens ( P = 0.766). Nine virus species and 349 viruses were isolated, 256 from NPS specimens and 273 from saliva specimens ( P = 0.1574). Adenovirus was detected more frequently in saliva samples ( P < 0.0001), whereas influenza virus type A and human rhinovirus were detected more frequently in NPS specimens ( P = 0.0001 and P = 0.0289, respectively). The possibility of false-positive adenovirus detection from saliva samples was excluded by direct sequencing. In conclusion, neither of the sampling methods was consistently more sensitive than the other. We suggest that these cost-effective methods for detecting respiratory viruses in mixed NPS-saliva specimens might be valuable for future studies.

scholarly journals Microfluidic sample preparation for respiratory virus detection: A review

2021 ◽  
Vol 15 (1) ◽  
pp. 011503
Author(s):  
Ryan Zenhausern ◽  
Chia-Hung Chen ◽  
Jeong-Yeol Yoon
Keyword(s):  
Sample Preparation ◽  
Respiratory Virus ◽  
Virus Detection

scholarly journals Combined SARS-CoV-2 nucleic acid amplification testing and respiratory virus panel RT-PCR on the Hologic Panther Fusion system

2021 ◽  
Vol 138 ◽  
pp. 104792
Author(s):  
Bryan A. Stevens ◽  
Catherine A. Hogan ◽  
Kenji O. Mfuh ◽  
Ghazala Khan ◽  
Malaya K. Sahoo ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Respiratory Virus ◽  
Nucleic Acid Amplification ◽  
Fusion System ◽  
Rt Pcr ◽  
Nucleic Acid Amplification Testing

scholarly journals Methods of Respiratory Virus Detection: Advances towards Point-of-Care for Early Intervention

Micromachines ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 697
Author(s):  
Siming Lu ◽  
Sha Lin ◽  
Hongrui Zhang ◽  
Liguo Liang ◽  
Shien Shen
Keyword(s):  
Viral Load ◽  
Respiratory Virus ◽  
Viral Infections ◽  
Point Of Care ◽  
Human Life ◽  
Low Cost ◽  
Virus Detection ◽  
Frequent Monitoring ◽  
User Friendly ◽  
New Strategies

Respiratory viral infections threaten human life and inflict an enormous healthcare burden worldwide. Frequent monitoring of viral antibodies and viral load can effectively help to control the spread of the virus and make timely interventions. However, current methods for detecting viral load require dedicated personnel and are time-consuming. Additionally, COVID-19 detection is generally relied on an automated PCR analyzer, which is highly instrument-dependent and expensive. As such, emerging technologies in the development of respiratory viral load assays for point-of-care (POC) testing are urgently needed for viral screening. Recent advances in loop-mediated isothermal amplification (LAMP), biosensors, nanotechnology-based paper strips and microfluidics offer new strategies to develop a rapid, low-cost, and user-friendly respiratory viral monitoring platform. In this review, we summarized the traditional methods in respiratory virus detection and present the state-of-art technologies in the monitoring of respiratory virus at POC.

Comparison of high throughput sequencing to standard protocols for virus detection in berry crops

Plant Disease ◽  
2021 ◽  
Author(s):  
Dan Edward Veloso Villamor ◽  
Karen E Keller ◽  
Robert Martin ◽  
Ioannis Emmanouil Tzanetakis
Keyword(s):  
High Throughput ◽  
High Throughput Sequencing ◽  
Wide Spectrum ◽  
Virus Detection ◽  
Rt Pcr ◽  
Host Interactions ◽  
Growing Seasons ◽  
Berry Crops ◽  
Sampling Times ◽  
Better Than

A comprehensive study comparing virus detection between high throughput sequencing (HTS) and standard protocols in 30 berry selections (12 Fragaria, 10 Vaccinium and 8 Rubus) with known virus profiles was completed. The study examined temporal detection of viruses at four sampling times encompassing two growing seasons. Within the standard protocols, RT-PCR proved better than biological indexing. Detection of known viruses by HTS and RT-PCR nearly mirrored each other. HTS provided superior detection compared to RT-PCR on a wide spectrum of virus variants and discovery of novel viruses. More importantly, in most cases where the two protocols showed parallel virus detection, 11 viruses in 16 berry selections were not consistently detected by both methods at all sampling points. Based on these data we propose a four sampling times/two-year testing requirement for berry and potentially other crops to ensure that no virus remains undetected independent of titer, distribution or other virus/virus or virus/host interactions.

scholarly journals Clinical significance of respiratory virus detection in patients with acute exacerbation of interstitial lung diseases

2018 ◽  
Vol 136 ◽  
pp. 88-92 ◽  
Author(s):  
Takeshi Saraya ◽  
Hirokazu Kimura ◽  
Daisuke Kurai ◽  
Masaki Tamura ◽  
Yukari Ogawa ◽  
...  
Keyword(s):  
Acute Exacerbation ◽  
Clinical Significance ◽  
Lung Diseases ◽  
Respiratory Virus ◽  
Virus Detection ◽  
Interstitial Lung Diseases

Rapid detection of different RNA respiratory virus species by multiplex RT-PCR: application to clinical specimens

1997 ◽  
Vol 8 (3) ◽  
pp. 227-232 ◽  
Author(s):  
Marcello Valassina ◽  
Anna Maria Cuppone ◽  
Maria Grazia Cusi ◽  
Piero Egisto Valensin
Keyword(s):  
Rapid Detection ◽  
Respiratory Virus ◽  
Virus Species ◽  
Rt Pcr ◽  
Clinical Specimens
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