Exogenous human immunodeficiency virus-1 protein, tat, enhances replication of JC virus efficiently in neuroblastoma cell lines

2012 ◽  
Vol 84 (4) ◽  
pp. 555-561 ◽  
Author(s):  
Souichi Nukuzuma ◽  
Masanori Kameoka ◽  
Shigeki Sugiura ◽  
Kazuo Nakamichi ◽  
Chiyoko Nukuzuma ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Cell Lines ◽  
Jc Virus ◽  
Neuroblastoma Cell ◽  
Immunodeficiency Virus ◽  
Neuroblastoma Cell Lines ◽  
Human Immunodeficiency Virus 1

Evidence for Involvement of Transforming Growth Factor β1 Signaling Pathway in Activation of JC Virus in Human Immunodeficiency Virus 1–Associated Progressive Multifocal Leukoencephalopathy

2004 ◽  
Vol 128 (3) ◽  
pp. 282-291
Author(s):  
Sahnila Enam ◽  
Thersa M. Sweet ◽  
Shohreh Amini ◽  
Kamel Khalili ◽  
Luis Del Valle
Keyword(s):  
Human Immunodeficiency Virus ◽  
Growth Factor ◽  
Progressive Multifocal Leukoencephalopathy ◽  
Transforming Growth Factor ◽  
Jc Virus ◽  
Transforming Growth Factor Β1 ◽  
Immunodeficiency Virus ◽  
Acquired Immunodeficiency ◽  
Immunodeficiency Syndrome ◽  
Human Immunodeficiency Virus 1

Abstract Context.—Progressive multifocal leukoencephalopathy is a fatal demyelinating disease of the central nervous system frequently seen in patients with impaired immune systems, particularly acquired immunodeficiency syndrome. JC virus (JCV), a human neurotropic polyomavirus, is the etiologic infectious agent of this disease. Objective.—The significantly higher incidence of progressive multifocal leukoencephalopathy in patients with acquired immunodeficiency syndrome than in patients with other immunosuppressive conditions suggests that molecular interactions between human immunodeficiency virus 1 and JCV, via the Tat protein, are responsible for the activation of the JCV enhancer/promoter and the development of progressive multifocal leukoencephalopathy. An indirect mechanism through activation of cytokines, such as transforming growth factor β1 and Smads 3 and 4, may also be responsible for the enhancement of JCV gene expression. Design.—Immunohistochemical analysis in progressive multifocal leukoencephalopathy samples and chloramphenicol acetyl transferase assays on cell cultures were performed to corroborate this hypothesis. Results.—The JCV capsid protein VP-1 was found in the nuclei of oligodendrocytes and in the nuclei and cytoplasm of bizarre astrocytes. Human immunodeficiency virus proteins, including p24 and Tat, were detected in the cytoplasm of astrocytes. Tat, but not p24, was detected in oligodendrocytes, suggesting that extracellular Tat accumulates in the nuclei of oligodendrocytes, where JCV gene transcription takes place. High levels of transforming growth factor β1 and Smads 3 and 4 were detected in JCV-infected oligodendrocytes. Results from in vitro studies confirm activation of the JCV early and late promoters by Smads 3 and 4. Conclusions.—These observations support our model, suggesting that the induction of transforming growth factor β1 by human immunodeficiency virus 1 Tat can stimulate its downstream factors, including Smads 3 and 4, which in turn augment transcription of the JCV promoter in glial cells.

scholarly journals JC virus/human immunodeficiency virus 1 co-infection in the Brazilian Amazonian region

2016 ◽  
Vol 20 (4) ◽  
pp. 360-364 ◽  
Author(s):  
Izaura Maria Vieira Cayres-Vallinoto ◽  
Antonio Carlos Rosário Vallinoto ◽  
Giselle Priscila dos Anjos Pena ◽  
Vânia Nakauth Azevedo ◽  
Luiz Fernando Almeida Machado ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Jc Virus ◽  
Immunodeficiency Virus ◽  
Amazonian Region ◽  
Human Immunodeficiency Virus 1

scholarly journals Increased Uptake and Accumulation of Vitamin C in Human Immunodeficiency Virus 1-infected Hematopoietic Cell Lines

1997 ◽  
Vol 272 (9) ◽  
pp. 5814-5820 ◽  
Author(s):  
Coralia I. Rivas ◽  
Juan Carlos Vera ◽  
Victor H. Guaiquil ◽  
Fernando V. Velásquez ◽  
Oriana A. Bórquez-Ojeda ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Vitamin C ◽  
Cell Lines ◽  
Hematopoietic Cell ◽  
Immunodeficiency Virus ◽  
Hematopoietic Cell Lines ◽  
Human Immunodeficiency Virus 1

Establishment of novel cell lines latently infected with human immunodeficiency virus 1

2011 ◽  
Vol 55 (2) ◽  
pp. 155-159 ◽  
Author(s):  
Y. OH ◽  
K. KIM ◽  
K. HONG ◽  
H. LEE ◽  
D. JANG ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Cell Lines ◽  
Immunodeficiency Virus ◽  
Latently Infected ◽  
Human Immunodeficiency Virus 1
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