Detection and typing of respiratory adenoviruses in a single-tube multiplex polymerase chain reaction

2002 ◽  
Vol 66 (4) ◽  
pp. 512-517 ◽  
Author(s):  
Byoung-Kuk Na ◽  
Jee-Hee Kim ◽  
Gu-Choul Shin ◽  
Joo-Yeon Lee ◽  
Jin-Soo Lee ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Multiplex Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Single Tube ◽  
Polymerase Chain

scholarly journals Application Of The Multiplex Polymerase Chain Reaction (M-PCR) For The Screening Of Vibrio Spp. From Rivers In Kuching, Sarawak

1970 ◽  
Vol 5 (2) ◽  
pp. 16-17
Author(s):  
Mickey Vincent ◽  
Lawrance Tuah ◽  
Christy Chan Sien Wei ◽  
Lesley Maurice Bilung ◽  
Kasing Apun
Keyword(s):  
Polymerase Chain Reaction ◽  
Vibrio Parahaemolyticus ◽  
Multiplex Polymerase Chain Reaction ◽  
Vibrio Vulnificus ◽  
Simultaneous Detection ◽  
Chain Reaction ◽  
Single Tube ◽  
Predominant Species ◽  
Polymerase Chain ◽  
Vibrio Spp

The present study was conducted to investigate the occurrence of Vibrio spp. from selected rivers in Kuching,Sarawak (Malaysia) using Multiplex Polymerase Chain Reaction (m-PCR). During the 6-month study period,19 samples were collected monthly from 7 rivers, followed by simultaneous detection of three Vibrio spp.,Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus, in a single tube PCR reaction. Three sets ofprimers targeting the thermolabile (tl), outer membrane protein (ompW) and hemolysin/cytolysin genes(vulCulsl) of V. parahaemolyticus, V. cholerae and V. vulnificus, respectively, were used. The results indicatedthat V. parahaemolyticus was the predominant species, occurring approximately 60.9% throughout thesampling period, followed by V. cholerae (23.1%) and V. vulnificus (16.0%). The months of July andDecember were found to be the months where all three Vibrio spp. were found to be at higher frequencies inthe river samples. Results analyzed also indicated that the rivers with the highest prevalence of the three Vibriospp. were Tambak Sejingkat, followed by Sungai Jernang and Sungai Tabuan. We conclude that m-PCR is apowerful and useful tool for the rapid and simultaneous detection of V. parahaemolyticus, V. cholerae and V.vulnificus from the riverine environments without the need for isolation and culturing. Furthermore, thismethod is highly specific, and could be applied in diagnostic laboratories for larger scale epidemiologicalinvestigations of Vibrio spp.

scholarly journals Molecular Detection of alpha Thalassemia: A Review of Prevalent Techniques

2021 ◽  
Author(s):  
Divashini Vijian ◽  
Wan Suriana Wan Ab Rahman ◽  
Kannan Thirumulu Ponnuraj ◽  
Zefarina Zulkafli ◽  
Noor Haslina Mohd Noor
Keyword(s):  
Polymerase Chain Reaction ◽  
Multiplex Polymerase Chain Reaction ◽  
Complete Blood Count ◽  
Globin Gene ◽  
Molecular Techniques ◽  
Chain Reaction ◽  
Single Tube ◽  
Advantages And Disadvantages ◽  
Alpha Thalassemia ◽  
Polymerase Chain

Alpha thalassemia (α-thalassemia) is an autosomal recessive disorder due to the reduction or absence of α globin chain production. Laboratory diagnosis of α-thalassemia requires molecular analysis for the confirmatory diagnosis. A screening test, comprising complete blood count, blood smear and hemoglobin quantification by high performance liquid chromatography and capillary electrophoresis, may not possibly detect all the thalassemia diseases. This review focused on the molecular techniques used to detect α-thalassemia, and the advantages and disadvantages of each technique were highlighted. Multiplex gap-polymerase chain reaction, single-tube multiplex polymerase chain reaction, multiplex ligation-dependent probe amplification, and loop-mediated isothermal amplification were used to detect common deletion of α-thalassemia. Furthermore, the reverse dot blot analysis and a single tube multiplex polymerase chain reaction could detect non-deletion mutation of the α-globin gene. Sanger sequencing is widely used to detect non-deletion mutations of α-thalassemia. Recently, next-generation sequencing was introduced in the diagnosis of both deletion and point mutations of α-thalassemia. Despite the advantages and disadvantages of different techniques, the routine method employed in the laboratory should be based on the facility, expertise, available equipment, and economic conditions.

scholarly journals Single-Tube Multiplex Polymerase Chain Reaction for the Detection of Genes Encoding Enterobacteriaceae Carbapenemase

2020 ◽  
Vol 73 (2) ◽  
pp. 166-172 ◽  
Author(s):  
Masanori Watahiki ◽  
Ryuji Kawahara ◽  
Masahiro Suzuki ◽  
Miyako Aoki ◽  
Kaoru Uchida ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Multiplex Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Single Tube ◽  
Genes Encoding ◽  
Polymerase Chain

A Rapid Single-Tube Multiplex Polymerase Chain Reaction Assay for the Seven Most Prevalent α-Thalassemia Deletions and αααanti 3.7α-Globin Gene Triplication

Hemoglobin ◽  
2010 ◽  
Vol 34 (2) ◽  
pp. 184-190 ◽  
Author(s):  
Arjan de Mare ◽  
Antoinette Heijs-Oude Groeneger ◽  
Sander Schuurman ◽  
Frank A.T.J.M. van den Bergh ◽  
Jennichjen Slomp
Keyword(s):  
Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Assay ◽  
Multiplex Polymerase Chain Reaction ◽  
Globin Gene ◽  
Chain Reaction ◽  
Single Tube ◽  
Polymerase Chain
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